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  4. Human Bradykinin,BK ELISA Kit

Human Bradykinin,BK ELISA Kit

  • 中文名稱：
    人血管舒緩激肽(BK)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09155h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血管舒緩激肽(BK)酶聯(lián)免疫試劑盒（CSB-E09155h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BK含量。試劑盒檢測范圍為0.312 pg/mL-20 pg/mL，適用于心血管疾病機(jī)制研究、炎癥相關(guān)信號(hào)通路探索、藥物干預(yù)效果評(píng)估等科研領(lǐng)域；為研究BK在血管內(nèi)皮功能調(diào)控、過敏性休克或局部水腫等病理模型中的作用提供有效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    BK
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 pg/mL-20 pg/mL
  • 靈敏度：
    0.078 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %93
    Range %90-95
    1:200Average %103
    Range %98-107
    1:400Average %93
    Range %86-97
    1:800Average %97
    Range %91-103
  • 回收率：
    The recovery of human BK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-97
    EDTA plasma (n=4)9793-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    202.586 2.566 2.576 2.467
    102.224 2.305 2.265 2.156
    51.711 1.681 1.696 1.587
    2.51.085 1.077 1.081 0.972
    1.250.624 0.630 0.627 0.518
    0.6250.379 0.368 0.374 0.265
    0.3120.243 0.232 0.238 0.129
    00.110 0.108 0.109 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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