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  4. Human Bromodomain testis-specific protein(BRDT) ELISA kit

Human Bromodomain testis-specific protein(BRDT) ELISA kit

  • 中文名稱：
    人溴結(jié)構(gòu)域睪丸特異蛋白(BRDT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL002807HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人溴結(jié)構(gòu)域睪丸特異蛋白(BRDT)酶聯(lián)免疫試劑盒（CSB-EL002807HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的BRDT含量。BRDT是一種溴結(jié)構(gòu)域蛋白。它與染色質(zhì)的動態(tài)變化、基因表達調(diào)控密切相關(guān)。在研究機制上，BRDT可通過識別乙?；M蛋白，調(diào)控精子發(fā)生等生理過程。目前，針對BRDT的研究旨在開發(fā)相關(guān)靶向藥物，用于癌癥、生殖疾病等的治療。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品適用于探索BRDT在睪丸發(fā)育、精子形成異?；蛏诚嚓P(guān)疾病模型中的表達變化，也可用于評估藥物或環(huán)境因素對生殖細胞表觀遺傳調(diào)控的影響，為生殖醫(yī)學(xué)基礎(chǔ)研究、雄性生育力評估及生殖毒性機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BRDT ELISA Kit; Bromodomain testis-specific protein ELISA Kit; Cancer/testis antigen 9 ELISA Kit; CT9 ELISA Kit; RING3-like protein ELISA Kit
  • 縮寫：
    BRDT
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BRDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-96
    1:2 Average % 87
    Range % 82-91
    1:4 Average % 105
    Range % 101-113
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of human BRDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 84 80-88
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.751 2.593 2.672 2.541
    2000 1.875 1.758 1.817 1.686
    1000 1.040 1.070 1.055 0.924
    500 0.592 0.611 0.602 0.471
    250 0.364 0.349 0.357 0.226
    125 0.239 0.227 0.233 0.102
    62.5 0.158 0.165 0.162 0.031
    0 0.127 0.134 0.131 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
  • 基因功能參考文獻：
    1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
    2. The bromodomain and extraterminal domain (BET) family consists of BRDT, BRD2, BRD3, and BRD4, each containing 2 bromodomains located N-terminal to extraterminal domain, which is located near C-terminus. Data suggest that 10 distinct acylations participate in binding of BET family proteins to histone 4 (oligopeptide fragments used here); C-terminal bromodomains do not cooperatively bind multiple acylation sites. PMID: 28945351
    3. The affected patient carries a homozygous bromodomain, testis-specific protein (BRDT) mutation, while the patient's father, mother and elder brother all carry heterozygous BRDT mutations. PMID: 28199965
    4. considered potential associations of 14 single nucleotide polymorphisms (SNPs) in RNF8 and BRDT genes in Chinese patients with non-obstructive azoospermia PMID: 25374327
    5. assessment of rs3088232 frequency in large group of non-obstructive azoospermia men and fertile controls demonstrated no significant difference between them; conclude that testicular impairments observed were not a consequence of BRDT gene mutation PMID: 24865796
    6. In human, BRDT is the only BET gene expressed exclusively in testicular germ cells. PMID: 22035730
    7. Suggest that reduced histone methylation in the promoter of BRDT may be associated with increased transcript levels in subfertile patients. PMID: 20538714
    8. BRDT-NY gene is an alternatively spliced variant that may have an important role in the process of spermatogenesis and may be correlated with male infertility PMID: 15647849

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  • 相關(guān)疾?。?/div>
    Spermatogenic failure 21 (SPGF21)
  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expressi
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1105

    OMIM: 602144

    KEGG: hsa:676

    STRING: 9606.ENSP00000354568

    UniGene: Hs.482520