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  4. Human Calcitonin,CT ELISA Kit

Human Calcitonin,CT ELISA Kit

  • 中文名稱：
    人降鈣素(CT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E05131h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人降鈣素(CT)酶聯(lián)免疫試劑盒（CSB-E05131h）檢測血清、血漿、組織勻漿樣本中的CALCA含量。CALCA 是重要靶點(diǎn)。它在機(jī)體生理活動里有關(guān)鍵作用。研究機(jī)制上，CALCA 參與多種信號通路，其表達(dá)異常和疾病發(fā)生發(fā)展關(guān)聯(lián)緊密?？蒲芯劢褂诮馕銎渥饔猛緩?，期望通過干預(yù) CALCA 開發(fā)治療疾病的新策略和藥物。試劑盒檢測范圍為4.688 pg/mL-300 pg/mL，該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中CT蛋白表達(dá)水平的定量檢測，如鈣代謝調(diào)控機(jī)制探索、骨骼疾病動物模型評估或甲狀腺功能相關(guān)病理研究，配套的血清/血漿前處理方案及組織勻漿制備指南可滿足不同實(shí)驗(yàn)體系需求，為科研人員提供穩(wěn)定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CALCA ELISA kit; CALC1Calcitonin [Cleaved into: Calcitonin; Katacalcin ELISA kit; Calcitonin carboxyl-terminal peptide ELISA kit; CCP ELISA kit; PDN-21)] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    4.688 pg/mL-300 pg/mL
  • 靈敏度：
    1.172 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 81-95  
    1:2 Average % 95  
    Range % 92-100  
    1:4 Average % 94  
    Range % 90-110  
    1:8 Average % 94  
    Range % 89-105  
  • 回收率：
    The recovery of human CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 85-100  
    EDTA plasma (n=4) 92 88-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    300 2.379 2.252 2.316 2.173  
    150 1.794 1.760 1.777 1.634  
    75 1.305 1.284 1.295 1.152  
    37.5 0.954 0.909 0.932 0.789  
    18.7 0.593 0.564 0.579 0.436  
    9.4 0.372 0.349 0.361 0.218  
    4.7 0.253 0.239 0.246 0.103  
    0 0.149 0.137 0.143 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.; Katacalcin is a potent plasma calcium-lowering peptide.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1437

    OMIM: 114130

    KEGG: hsa:796

    STRING: 9606.ENSP00000331746

    UniGene: Hs.37058