Human Calponin-2(CNN2) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人鈣調(diào)蛋白2(CNN2)酶聯(lián)免疫試劑盒（CSB-EL005656HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CNN2含量。人鈣調(diào)蛋白2（CNN2）是一種在細胞信號傳導中起關鍵作用的蛋白質，其研究背景涉及其在細胞內(nèi)信號傳遞中的角色。CNN2的機制主要涉及調(diào)節(jié)鈣離子濃度，影響平滑肌收縮，與心血管疾病和神經(jīng)生物學相關。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外培養(yǎng)細胞模型、動物組織樣本或體液樣本的蛋白表達分析，為探索 CNN2 在細胞應激響應、組織再生機制或疾病相關分子通路中的功能提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Calponin 2 ELISA Kit; Calponin H2 ELISA Kit; Calponin H2, smooth muscle ELISA Kit; Calponin-2 ELISA Kit; Cnn2 ELISA Kit; CNN2_HUMAN ELISA Kit; Neutral calponin ELISA Kit; smooth muscle ELISA Kit
縮寫：

CNN2
Uniprot No.：

Q99439
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CNN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	98
	Range %	93-103
1:4	Average %	95
	Range %	89-98
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of human CNN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-106
EDTA plasma (n=4)	94	86-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
基因功能參考文獻：
1. Study in human hepatocellular carcinoma cell line and xenograft mouse model shows that CNN2 plays an important role in tumor growth and metastasis, possibly through MEK1/2-ERK1/2 signaling pathway. PMID: 29333089
2. our results demonstrated that knockdown of endogenous CNN2 in AGS cells could significantly activate cell apoptosis pathway and therefore suppress cell growth in vitro. The deletion of CNN2 might be a potential therapeutic approach to inhibit aggressive growth of gastric cancer. PMID: 28714360
3. High Calponin-h2 serum levels are associated with breast cancer. PMID: 25099617
4. h2-Calponin gene expression is further affected by the treatment of cells with Notch inhibitor and activator, suggesting an upstream signaling mechanism. PMID: 24285540
蛋白家族：

Calponin family
組織特異性：

Heart and smooth muscle.
數(shù)據(jù)庫鏈接：

HGNC: 2156

OMIM: 602373

KEGG: hsa:1265

STRING: 9606.ENSP00000263097

UniGene: Hs.651512