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  4. Human Carboxymethyl lysine,CML ELISA Kit

Human Carboxymethyl lysine,CML ELISA Kit

  • 中文名稱：
    人羧甲基賴氨酸(CML)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12798h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人羧甲基賴氨酸(CML)酶聯(lián)免疫試劑盒（CSB-E12798h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CML含量。CML即慢性髓系白血病，是一種起源于多能造血干細(xì)胞的血液系統(tǒng)惡性克隆性疾病，與費(fèi)城染色體及BCR-ABL融合基因相關(guān)。研究機(jī)制聚焦于該基因異常導(dǎo)致的酪氨酸激酶持續(xù)活化，目前多通過靶向抑制該激酶來開展治療。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于基礎(chǔ)科研中AGEs代謝機(jī)制探索、抗衰老藥物功效評估以及糖尿病相關(guān)動(dòng)物模型或細(xì)胞實(shí)驗(yàn)的分子標(biāo)記物檢測。其寬泛的樣本兼容性支持跨組織類型的比較研究，為營養(yǎng)干預(yù)、代謝性疾病機(jī)理及氧化損傷相關(guān)領(lǐng)域提供可靠工具。助力研究者深入解析CML在慢性疾病發(fā)生發(fā)展中的生物學(xué)作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    CML
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CML in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-95
    1:2Average %94
    Range %90-100
    1:4Average %95
    Range %91-105
    1:8Average %94
    Range %85-100
  • 回收率：
    The recovery of human CML spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-98
    EDTA plasma (n=4)9491-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.372 2.252 2.312 2.162
    20001.775 1.660 1.718 1.568
    10001.160 1.142 1.151 1.001
    5000.712 0.699 0.706 0.556
    2500.493 0.456 0.475 0.325
    1250.342 0.329 0.336 0.186
    62.50.264 0.249 0.257 0.107
    00.153 0.147 0.150 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

Does?this?kit?measure?the?total?or?protein?bound?cml?

A:
Thanks for your inquiry. CSB-E12798h detects protein bound CML.Pls let me know if you have any further questions. Thank you.
Q:

We have planned to assess the abundance of cml in protein samples, collected from cell cultures, using the human CML ELISA kit CSB-E12798h. What kind of lysis buffer is recommended to use to collect CML in cell samples? We are used to lyse cell with T-PER/Protease inhibitor cocktail or Igepal at 0.5%. Are they compatible with the purchased CML Elisa kit (CSB-E12798h)

A:
Thanks for your inquiry.
From your description, you want to make detection on cell lysis, here advice you to use the sample method on our attached manual.
As for the method you mentioned, so sorry that it is not verified from our side.
If your sample has been prepared, pls arrange preliminary experiment to check.
The enzyme proportion should be combined with the enzyme preparation protocol.
And dear, we have 24T trial kit, if you have interest, advice you to have this small size for try.
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.