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  4. Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

  • 中文名稱：
    人羧基肽酶N亞基2(CPN2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005899HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人羧基肽酶N亞基2(CPN2)酶聯(lián)免疫試劑盒（CSB-EL005899HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CPN2含量。人羧基肽酶N亞基2（CPN2）是一種羧肽酶，參與蛋白質(zhì)降解和細(xì)胞信號(hào)傳導(dǎo)。其研究機(jī)制涉及CPN2在蛋白質(zhì)降解過程中的作用，以及如何通過調(diào)節(jié)細(xì)胞內(nèi)信號(hào)通路影響細(xì)胞功能。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于基礎(chǔ)科研中探索CPN2在心血管疾病、免疫調(diào)節(jié)或炎癥機(jī)制中的生物學(xué)功能，亦可用于體外模型中蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CPN2 ELISA kit; ACBPCarboxypeptidase N subunit 2 ELISA kit; Carboxypeptidase N 83 kDa chain ELISA kit; Carboxypeptidase N large subunit ELISA kit; Carboxypeptidase N polypeptide 2 ELISA kit; Carboxypeptidase N regulatory subunit ELISA kit
  • 縮寫：
    CPN2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CPN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    99

    Range %

    92-105

    1:2

    Average %

    106

    Range %

    102-112

    1:4

    Average %

    97

    Range %

    91-103

    1:8

    Average %

    93

    Range %

    89-98

  • 回收率：

    The recovery of human CPN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-101

    EDTA plasma (n=4)

    97

    93-103

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.858

    2.934

    2.896

    2.755

    5

    2.131

    2.167

    2.149

    2.008

    2.5

    1.275

    1.328

    1.302

    1.160

    1.25

    0.837

    0.829

    0.833

    0.692

    0.625

    0.492

    0.521

    0.506

    0.365

    0.312

    0.317

    0.323

    0.320

    0.179

    0.156

    0.238

    0.244

    0.241

    0.100

    0

    0.137

    0.145

    0.141

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
  • 基因功能參考文獻(xiàn)：
    1. Real-time PCR and western blotting show the following CPN2 expression: minimal in MDA-MB-468 cells but obvious in MDA-MB-231 and MCF7 cells. MDA-MB-231 breast cancer cell lines were selected for the control group and CPN2 was knocked out for the experimental group. PMID: 26860443
  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2313

    OMIM: 603104

    KEGG: hsa:1370

    STRING: 9606.ENSP00000319464

    UniGene: Hs.528368