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  4. Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

  • 中文名稱：
    人抗菌肽(CAMP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL004476HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人抗菌肽(CAMP)酶聯(lián)免疫試劑盒（CSB-EL004476HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CAMP含量。CAMP（環(huán)腺苷酸）是細(xì)胞內(nèi)重要的第二信使，參與多種生理過程。研究顯示，失控的cAMP可能導(dǎo)致肝癌，其機(jī)制涉及cAMP在無膜細(xì)胞器中的動態(tài)隔離，當(dāng)這些結(jié)構(gòu)被破壞時(shí)，cAMP會淹沒細(xì)胞，導(dǎo)致失控生長。試劑盒檢測范圍為0.45 ng/mL-30 ng/mL，適用于體外科研實(shí)驗(yàn)中探索CAMP在感染性病原體應(yīng)答、慢性炎癥性疾?。ㄈ玢y屑病、炎癥性腸?。┗蚱つw創(chuàng)傷愈合等生理病理過程中的表達(dá)變化，為免疫機(jī)制研究及潛在生物標(biāo)志物分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37,
  • 縮寫：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.45 ng/mL-30 ng/mL
  • 靈敏度：
    0.11 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 88  
    Range % 80-92  
    1:200 Average % 96  
    Range % 91-105  
    1:400 Average % 99  
    Range % 92-110  
    1:800 Average % 95  
    Range % 86-98  
  • 回收率：
    The recovery of human CAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 89-98  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    30 2.713 2.664 2.689 2.612  
    15 1.687 1.567 1.627 1.550  
    7.5 1.047 0.989 1.018 0.941  
    3.75 0.563 0.543 0.553 0.476  
    1.8 0.339 0.324 0.332 0.255  
    0.9 0.206 0.196 0.201 0.124  
    0.45 0.145 0.139 0.142 0.065  
    0 0.080 0.074 0.077 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

we are interested in measurements of the Cathelicidin antimicrobial peptide (CAMP) in human fecal samples. According to your homepage, your assay is suitable for blood samples or tissue homogenates. But have you ever tested it in fecal samples? Would you expect it to work using fecal samples?

A:
Thanks for your inquiry!
CSB-EL004476HU
We tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test human fecal samplee ,we suggest you do a pretest . Pls find following sample preparation method.
Feces Dilute samples with appropriate Sample Diluent. This can be achieved by adding 0.1g sample to appropriate Sample Diluent. Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.
Pls let me know if you have any further questions. Thank you.