Human Cathepsin G(cath-G)ELISA Kit
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中文名稱:人組織蛋白酶G(cath-G)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E16388h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人組織蛋白酶G(cath-G)酶聯(lián)免疫試劑盒(CSB-E16388h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的CTSG含量。CTSG為一種重要靶點(diǎn)。它在人體多種生理病理過程中有作用。研究發(fā)現(xiàn)其參與炎癥反應(yīng)、免疫調(diào)節(jié)等機(jī)制。深入探索CTSG的作用機(jī)制,有助于揭示相關(guān)疾病發(fā)病原理,為開發(fā)針對(duì)性治療藥物、制定治療方案提供理論依據(jù)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL,研究者可借助探索中性粒細(xì)胞相關(guān)炎癥反應(yīng)機(jī)制、感染性疾病病理過程或免疫調(diào)控網(wǎng)絡(luò)中的分子作用,為炎癥性疾病、感染免疫及細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)等領(lǐng)域的機(jī)制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:CATG ELISA Kit; CATG_HUMAN ELISA Kit; Cathepsin G ELISA Kit; Cathepsin G precursor ELISA Kit; CathepsinG ELISA Kit; CG ELISA Kit; Ctsg ELISA Kit; CTSG protein ELISA Kit; MGC23078 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.8 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human cath-G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 92 Range % 80-99 1:10 Average % 97 Range % 91-105 1:20 Average % 97 Range % 88-110 1:40 Average % 93 Range % 85-98 -
回收率:
The recovery of human cath-G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 96 91-100 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.617 2.654 2.636 2.512 1000 2.205 2.254 2.230 2.106 500 1.636 1.705 1.671 1.547 250 0.943 0.989 0.966 0.842 125 0.509 0.554 0.532 0.408 62.5 0.352 0.369 0.361 0.237 31.25 0.226 0.241 0.234 0.110 0 0.122 0.126 0.124 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Cleaves vimentin. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa.
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基因功能參考文獻(xiàn):
- CTSG was increased in peripheral blood mononuclear cells and muscle tissues of dermatomyositis (DM) patients, and CTSG activity was higher in the serum of DM patients PMID: 28797985
- CatG can be used as a novel marker to distinguish different NK cell subsets and MARS116 is applicable to determine cell surface CatG activity by flow cytometry. PMID: 27666013
- Results provide evidence that cathepsin G (GC) activates IGF-1R by stimulating IGF-1 release from MCF-7 cells, and that IGF-1R activation is required for CG-induced cell aggregation. PMID: 28544544
- Cleavage of the alarmins by Human mast cell chymase and human neutrophil cathepsin G suggests a function in regulating excessive inflammation. PMID: 28053237
- Data show that lactoferrin (LF) increases the catalytic activity of cathepsin G (CatG) at physiological concentration. PMID: 26986619
- CatG is an essential protease for regulating MHC I molecules PMID: 26837514
- Patients with certain polymorphisms in the CTSG gene had lower risk for chronic postsurgical pain compared with wild-types. PMID: 26270939
- These in vivo data provide, for the first time, compelling evidence of the collateral involvement of cathepsin G, NE, and proteinase 3 in cigarette smoke-induced tissue damage and emphysema PMID: 24929239
- expression levels of ELANE and CTSG were determined by quantitative real-time PCR PMID: 25248056
- Elastase and cathepsin G are elevated in the plasma of HD patients, originating from primed PMNLs. In these patients, chronic elevation of these enzymes contributes to cleavage of VE-cadherin and possible disruption of endothelial integrity. PMID: 24877096
- Cathepsin G is an antimicrobial protein with bacteriocidal activity against S. aureus and N. gonorrhoeae. PMID: 2116408
- proteolytic cleavage of PLTP by cathepsin G may enhance the injurious inflammatory responses that occur in COPD PMID: 24532668
- Neutrophil cathepsin G is a physiologic modulator of platelet thrombus formation in vivo and has potential as a target for novel anti-thrombotic therapies. PMID: 23940756
- demonstrate that cathepsin G (CG), neutrophil elastase (NE), and to a lesser extent proteinase 3 (PR3), degrade endocan PMID: 23454598
- A novel HLA-A*0201 restricted peptide derived from cathepsin G is an effective immunotherapeutic target in acute myeloid leukemia. PMID: 23147993
- the targeting and suppression of CTSG by AML1-ETO in t(8;21) AML may provide a mechanism for leukemia cells to escape from the intracellular surveillance system by preventing degradation of foreign proteins. PMID: 22641217
- NE and CG bind to the surface of cancer cells, presumably to a cell surface receptor, and subsequently undergo clathrin pit-mediated endocytosis. PMID: 22915586
- major binding partners of LEKTI were found to be the antimicrobial peptide dermcidin and the serine protease cathepsin G and no kallikreins. PMID: 22588119
- Data suggested the notion that CatG plays a critical role in proinsulin processing and is important in the activation process of diabetogenic T cells. PMID: 21850236
- potential of HNE and CatG to be used as markers for early detection of infection. Significant differences in HNE and CatG levels in infected and non-infected wound fluids were observed. PMID: 21488974
- CatG was found to be dispensable in invariant chain conversion within intact primary human B cells and dendritic cells. PMID: 21543057
- distinct catK/C4-S interactions are necessary for the collagenolytic activity of the enzyme. PMID: 21193413
- Neutrophil cathepsin G can either facilitate or impede coagulation via effects on thrombin generation. PMID: 20589323
- CatG cleaves human leukocyte antigen (HLA)-DR in vitro. Cleavage occurred on the loop between fx1 and fx2 of the membrane-proximal beta2 domain. In vivo, however, the CatG cleavage site is sterically inaccessible or masked by associated molecules. PMID: 20331476
- Historical and recent data on CatG expression, distribution, function and involvement in disease will be summarized and discussed, with a focus on its role in antigen presentation and immune-related events. PMID: 19910052
- high molecular mass kininogen down-regulates cathepsin G-induced platelet activation by forming a complex with cathepsin G and thus prevents binding of cathepsin G to platelets PMID: 11920276
- UVA light stimulates the production of cathepsin G and elastase-like enzymes by dermal fibroblasts: a possible contribution to the remodeling of elastotic areas in sun-damaged skin. PMID: 11928814
- up-regulated by the AML1-MTG8 fusion protein, suggesting a role in the granulocytic maturation characteristic of the t(8;21) acute myelogenous leukemia PMID: 11986950
- cathepsin G enhances fibrin deposition under flow conditions by elevating the activation state of fibrinogen-adherent platelets rather than by cleaving coagulation factors PMID: 12524437
- BPI and cathepsin G are the major antigenic targets of ANCA seen in patients with systemic sclerosis PMID: 12784398
- Serine proteinases cathepsin G and neutrophil elastase cooperate for the proteolytic regulation of CD87/urokinase receptor on monocytic cells. PMID: 14688365
- role in development of myelodi leukemia with promyelocytic features PMID: 14737102
- Cathepsin G initializes and dominates the destruction of intact myelin basic protein (MBP) by B cell-derived lysosomal extracts, degrading immunodominant MBP epitope and eliminating both its binding to HLA-DRB1*1501 and MBP-specific T cell response. PMID: 15100291
- neutrophil elastase and cathepsin G are inhibited by PAI-1 mutants PMID: 15131125
- Release of cathepsin G from neutrophils specifically down-regulates the responsiveness of neutrophils to C5a, and this effect may play a role in the down-regulation of acute inflammation. PMID: 15140022
- down-regulation of CatG in macrophages is advantageous to M. tuberculosis bacilli and possibly is an important mechanism by which M. tuberculosis is able to evade the host immune defenses PMID: 15385470
- oxidants generated by myeloperoxidase regulate cathepsin G activity in vivo PMID: 15967795
- Neutrophil cathepsin G is the principal protease to produce CCL15 proteolytic products released in the course of hemofiltration of blood from renal insufficiency patients PMID: 16034099
- PF-4 acts as an inhibitor of the CTAP-III-processing enzymes cathepsin G and chymase without becoming cleaved itself as a competitive substrate. PMID: 16317101
- the monocyte chemotactic activity of cathepsin G may have a role in the pathogenesis of RA synovial inflammation PMID: 16977463
- cathepsin g may play a role in the progression to heart failure by activating angiotensin II, leading to detrimental effects on the heart PMID: 17418861
- Substrate specificity for cathepsin G is greatly enhanced when an aromatic side chain and a strong positive charge are incorporated in residue P(1). PMID: 17653609
- Cathepsin G from neutrophils and monocytes may provide some pro-coagulant effect by activating FVIII. PMID: 18217133
- The cleavage of neutrophil leukosialin (CD43) by cathepsin G releases its extracellular domain and triggers its intramembrane proteolysis by presenilin/gamma-secretase PMID: 18586676
- Cathepsin G increases MMP expression in normal human fibroblasts through fibronectin fragmentation, and induces the conversion of proMMP-1 to active MMP-1. PMID: 18835135
- Data report that the two subsets of human dendritic cells differ in their cathepsin distribution, and suggest tha Cathepsin G may be of functional importance. PMID: 19036358
- MC are immunoreactive to cathepsin-G in human cutaneous mastocytosis, as well as the co-localization of tryptase and cathepsin-G in MC secretory granules. PMID: 19250736
- both Cat-G and PAR(4) play key roles in generating and/or amplifying relapses in ulcerative colitis PMID: 19528350
- Cathepsin G is one of the mediators responsible for complement-dependent opsonophagocytic killing of Streptococcus pneumoniae by human neutrophils. PMID: 19620298
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亞細(xì)胞定位:Cell surface. Membrane; Peripheral membrane protein.
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蛋白家族:Peptidase S1 family
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