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  4. Human Cementum Protein 1 (CEMP-1) ELISA Kit

Human Cementum Protein 1 (CEMP-1) ELISA Kit

  • 中文名稱：
    人牙骨質(zhì)蛋白1（CEMP-1）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13012h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人牙骨質(zhì)蛋白1（CEMP-1）酶聯(lián)免疫試劑盒（CSB-E13012h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的CEMP1含量。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品適用于體外實(shí)驗(yàn)研究，可用于探索牙周炎病理機(jī)制、評(píng)估牙骨質(zhì)再生治療方案的療效，或作為骨代謝相關(guān)疾病的分子機(jī)制研究工具，為口腔醫(yī)學(xué)、骨生物學(xué)等領(lǐng)域的科研工作者提供精準(zhǔn)的蛋白定量解決方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit
  • 縮寫：
    CEMP1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-95
    1:2Average %101
    Range %97-105
    1:4Average %83
    Range %80-86
    1:8Average %107
    Range %103-110
  • 回收率：
    The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-96
    EDTA plasma (n=4)9184-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.658 2.697 2.678 2.528
    52.312 2.331 2.322 2.172
    2.51.871 1.892 1.882 1.732
    1.251.312 1.365 1.339 1.189
    0.6250.788 0.797 0.793 0.643
    0.3120.442 0.472 0.457 0.307
    0.1560.281 0.272 0.277 0.127
    00.151 0.149 0.150
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May play a role in development of the periodontium which surrounds and supports the teeth by promoting the differentiation of multi-potent cells from the periodontal ligament into cementoblasts to form the cementum. Binds hydroxyapatite and may promote the biomineralization of the cementum. Also promotes cell proliferation.
  • 基因功能參考文獻(xiàn)：
    1. The synthesis of hydroxyapatite with different crystallinities by controlling the concentration of recombinant CEMP1 for biological application. PMID: 26652387
    2. CEMP1 exerts modulation of a number of cellular genes. PMID: 26011628
    3. when HIF-1 was activated by gene introduction or chemically, CEMP1 expression and mineralization increased. PMID: 24117017
    4. human primary cementoblasts subjected to compression and IL-1beta stimulation impeded BSP and CEMP-1 expression, proteins that are associated with cementogenesis. PMID: 22349547
    5. These data demonstrate for the first time that the CEMP1 is not only a marker protein for cementoblast-related cells, but it also regulates cementoblast commitment in periodontal ligament cells PMID: 21465469
    6. CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and it might have a potential function in cementum and bone formation PMID: 17509525
    7. CEMP1 plays a role during the biomineralization process by promoting octacalcium phosphate crystal nucleation. PMID: 19393626

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 組織特異性：
    Expressed by cementoblasts, a subpopulation of periodontal ligament cells and cells located around vessels in periodontium (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 32553

    OMIM: 611113

    KEGG: hsa:752014

    STRING: 9606.ENSP00000371787

    UniGene: Hs.433499