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  4. Human Ciliary Neurotrophic Factor,CNTF ELISA Kit

Human Ciliary Neurotrophic Factor,CNTF ELISA Kit

  • 中文名稱：
    人睫狀神經(jīng)營養(yǎng)因子(CNTF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04527h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人睫狀神經(jīng)營養(yǎng)因子(CNTF)酶聯(lián)免疫試劑盒（CSB-E04527h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CNTF含量。CNTF（睫狀神經(jīng)營養(yǎng)因子）是一種由睫狀神經(jīng)營養(yǎng)因子基因編碼的蛋白質(zhì)，屬于IL-6細(xì)胞因子家族。它具有神經(jīng)營養(yǎng)和分化作用，能促進(jìn)神經(jīng)遞質(zhì)的合成和某些神經(jīng)元群的軸突生長。CNTF在神經(jīng)保護(hù)和視網(wǎng)膜神經(jīng)節(jié)細(xì)胞存活中發(fā)揮重要作用，是青光眼等神經(jīng)退行性疾病研究的熱點靶點。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，本產(chǎn)品適用于體外實驗研究CNTF在神經(jīng)系統(tǒng)、代謝調(diào)節(jié)或炎癥反應(yīng)中的作用機制，可應(yīng)用于神經(jīng)生物學(xué)、細(xì)胞信號通路研究以及疾病動物模型中神經(jīng)營養(yǎng)因子水平的動態(tài)監(jiān)測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ciliary neurotrophic factor ELISA Kit; CNTF ELISA Kit; CNTF_HUMAN ELISA Kit; HCNTF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CNTF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 83-91  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 99  
    Range % 95-103  
    1:8 Average % 86  
    Range % 83-89  
  • 回收率：
    The recovery of human CNTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-103  
    EDTA plasma (n=4) 90 85-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.504 2.598 2.551 2.369  
    200 2.194 2.053 2.124 1.942  
    100 1.555 1.501 1.528 1.346  
    50 0.877 0.898 0.888 0.706  
    25 0.451 0.465 0.458 0.276  
    12.5 0.375 0.362 0.369 0.187  
    6.25 0.274 0.298 0.286 0.104  
    0 0.181 0.183 0.182    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
  • 基因功能參考文獻(xiàn)：
    1. Our findings suggest that CNTF over-expression enhances the protective effects of bone marrow mesenchymal stem cells on retinal pigment epithelium cells, thus indicating subretinal-transplantation of CNTF-bone marrow mesenchymal stem cells may be a promising therapy for blue-light -injured retina PMID: 29564604
    2. Cytokines of the LIF/CNTF family and metabolism PMID: 26817395
    3. High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration. PMID: 28323167
    4. CNTFR-specific mutants of CNTF have been developed that bind to the CNTFRalpha-LIFRbeta-gp130 receptor. PMID: 26187860
    5. Human CNTF expression through lentiviral gene transfer in the rat striatum significantly decreased the levels of neuronal metabolites (N-acetyl-aspartate, N-acetyl-aspartyl-glutamate, and glutamate). PMID: 25833344
    6. the biological effects of CNTF on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells and the underlying molecular mechanism of this effect were investigated for the first time PMID: 25118897
    7. R28E mutation in CNTF abrogatesIL-6 receptor-dependent but retains CNTF receptor-dependent signaling via glycoprotein 130/ LIFR. PMID: 24802752
    8. In this review, CNTF plays an important role in neurogenesis and differentiation of neural stem cells. PMID: 23948898
    9. The levels of expression and secretion of BDNF and CTNF of induced cells were assessed using immunocytochemical, Real-Time polymerase chain reaction, and enzyme linked immunosorbent assay (ELISA). PMID: 23944834
    10. CNTF elevated in umbilical cord blood from pre-eclamptic pregnancies PMID: 23654315
    11. CNTF-mediated protection of photoreceptors requires initial activation of the cytokine receptor gp130 in Muller glial cells. PMID: 24191003
    12. Report favourable allelic patterns of ACTN3 and CNTF genes on aerobic performance in athletes. PMID: 23676962
    13. Data indicate that aspirin increased mRNA and protein expression of CNTF in primary mouse and human astrocytes in a dose- and time-dependent manner. PMID: 23653362
    14. we show that the release of CNTF by glial cells is a very powerful stimulus for optic fiber regeneration and retinal ganglion cell survival after optic nerve crush PMID: 23194670
    15. Ciliary neurotrophic factor levels were increased in painful PTT only. PMID: 22337942
    16. Peptide 6c, corresponding to human CNTF amino acid residues 147-150, induces neurogenesis, neuronal activity and proliferation of immature neurons in the dentate gyrus and improvement of spatial reference memory in mice. PMID: 20952820
    17. CNTF's effects on retinal pigment epithelial (RPE) physiology PMID: 21912637
    18. Data show that variants in CNTF were significantly associated with a lower age at onset of the eating disorders. PMID: 20219210
    19. The role played by CNTF in retinal cell differentiation and survival in retinal progenitors, is reported. PMID: 20428961
    20. Ciliary neurotrophic factor, and related (CNTF), ligands targeting the established CNTF receptor alpha, binds to sortilin with high affinity. PMID: 20584990
    21. In women the CNTF polymorphism (odds ratio (OR) = 2.15, 95%CI: 1.27-3.64, p = 0.004) are associated with weight gain. PMID: 19833146
    22. Studies indicate that leptin, CNTF, LIF and IL-6 present similar three-dimensional fold structure, interact with related class-I receptors and activate similar intracellular pathways. PMID: 19751193
    23. A null mutation in this protein was evaluated for a relationship to disease susceptibility and disease severity in patients with multiple sclerosis. PMID: 11857064
    24. Association of a null mutation in the CNTF gene with early onset of multiple sclerosis. PMID: 11890844
    25. Early onset of severe familial amyotrophic lateral sclerosis with a SOD-1 mutation: potential impact of CNTF as a candidate modifier gene. PMID: 11951178
    26. CTNF binds to the IL-6 receptor and has a role in neuroprotection PMID: 12643274
    27. no evidence found to suggest that ciliary neurotrophic factor is involved in the pathogenesis of pelvic endometriosis PMID: 12890930
    28. Constitutive expression of cytokines in brain induces changes in gene expression characteristic of chronic inflammation leading to either temporal weight reduction (CNTF) or severe cachexia (leukemia inhibitory factor). PMID: 14715713
    29. Results do not support an effect of the CNTF null allele on body composition, contrary to previous findings. PMID: 14747836
    30. findings support the hypothesis that CNTF and leptin engage distinct CNS sites and CNTF possesses inflammatory properties distinct from leptin PMID: 15047605
    31. In spite of axokine gene expression in retinal pigment epithelium, no photoreceptor rescue in retinal degeneration mice. PMID: 15180291
    32. myogenic lineage-committed human myoblasts can dedifferentiate at a clonal level; CNTF is a novel regulator of skeletal myoblast dedifferentiation via p44/p42 MAPK pathway PMID: 15843428
    33. CNTF negatively regulates phototransduction, which reduces the photoresponsiveness of rods, resulting in lower electroretinogram amplitudes following light stimulus. PMID: 17192435
    34. Possible neuroprotective role of CNTF in the optic nerve head. PMID: 17563726
    35. We conclude that absence of CNTF does not increase susceptibility for neurodegenerative disorders and confirm that it does not affect onset and course of familial and sporadic ALS. PMID: 17651970
    36. Continuous expression of striatal CNTF at the dose mediated by the expression cassette used in this study was detrimental to transgenic mice with Huntington's disease. PMID: 18293418
    37. The relative treatment benefit of iloperidone compared with placebo in patients with schizophrenia is enhanced in patients homozygous G/G for the rs1800169 polymorphism of CNTF. PMID: 18303965
    38. In vivo and in vitro experiments implicate CNTF as an endogenous regulatory component of dopamine D2-receptor-dependent neurogenesis in the subventricular zone and the dentate gyrus of the hippocampus. (Review) PMID: 18524890
    39. CNTF-mediated signaling is a molecular switch for neuronal versus glial differentiation of retinal stem cells/progenitors. PMID: 18669911
    40. Ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin share a conserved binding site on the ciliary neurotrophic factor receptor alpha chain PMID: 18728012
    41. PTP-1B constitutes a key divergent element between leptin/insulin and CNTF signaling pathways at the neuronal level. PMID: 19008309
    42. Certain SNP patterns in VDR and CNTF genes showed better improvement of parameters associated with the effects of low-resistance training using exercise machines as analyzed by comparison between SNP patterns and factor analysis. PMID: 19082510
    43. The results of this study provided further evidence that the production of ciliary neurotrophic factor by Schwann cells is markedly reduced in Charcot-Marie-Tooth type 1A neuropathy. PMID: 19525893
    44. Fusion of HIV-1 TAT to CNTF may have modified the CNTF capacity to induce intracellular signaling in hypothalamic neurons. PMID: 19573019
    45. The CNTF 1357 G --> A polymorphism explains only a small portion of the variability in the muscle strength response to training in women. PMID: 19628720
    46. An interaction between apoE3 and CNTF occurs with both delipidated apeE3 and apoE3 found within a lipoprotein particle in cerebrospinal fluid. This interaction potentiates the survival-promoting activity of CNTF for hippocampal neurons. PMID: 9236223

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    CNTF family
  • 組織特異性：
    Nervous system.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2169

    OMIM: 118945

    KEGG: hsa:1270

    STRING: 9606.ENSP00000355370

    UniGene: Hs.524920