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  4. Human Clara cell protein,CC16 ELISA Kit

Human Clara cell protein,CC16 ELISA Kit

  • 中文名稱：
    人克拉拉細胞蛋白(CC16)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08680h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人克拉拉細胞蛋白(CC16)酶聯(lián)免疫試劑盒（CSB-E08680h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CC16含量。CC16，即克拉拉細胞分泌蛋白16，是一種由呼吸道上皮的club細胞分泌的蛋白質(zhì)，具有抗炎、免疫調(diào)節(jié)和抗纖維化等多種生物學功能。研究發(fā)現(xiàn)，CC16在慢性氣道疾病的發(fā)病機制中起到重要作用，可能是慢性氣道疾病的潛在生物標志物和治療靶點。其抗炎機制包括抑制促炎因子、阻斷NF-κB信號通路和抑制PLA2的活性。試劑盒檢測范圍為0.625 ng/ml- 40 ng/ml，靈敏度為0.156 ng/ml。適用于基礎科研中探索肺部疾病機制、評估環(huán)境污染物或藥物對肺功能的影響、以及相關動物模型研究中的生物標志物分析；為呼吸系統(tǒng)疾病研究、毒理學實驗及生物醫(yī)學機制探索提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Blastokinin ELISA Kit; CC10 ELISA Kit; CC16 ELISA Kit; CCPBP ELISA Kit; CCSP ELISA Kit; Clara cell phospholipid binding protein ELISA Kit; Clara cell phospholipid-binding protein ELISA Kit; Clara cell specific 10 kD protein ELISA Kit; Clara cells 10 kDa secretory protein ELISA Kit; OTTHUMP00000236107 ELISA Kit; SCGB1A1 ELISA Kit; Secretoglobin family 1A member 1 ELISA Kit; Secretoglobin, family 1A, member 1 (uteroglobin) ELISA Kit; UG ELISA Kit; UGB ELISA Kit; UP-1 ELISA Kit; UP1 ELISA Kit; Urinary protein 1 ELISA Kit; Urine protein 1 ELISA Kit; UTER_HUMAN ELISA Kit; Uteroglobin ELISA Kit
  • 縮寫：
    CC16
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/ml - 40 ng/ml
  • 靈敏度：
    0.156 ng/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CC16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:20

    Average %

    104

    Range %

    97-110

    1:40

    Average %

    98

    Range %

    91-106

    1:80

    Average %

    99

    Range %

    92-105

    1:160

    Average %

    90

    Range %

    86-97

  • 回收率：

    The recovery of human CC16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    92-100

    EDTA plasma (n=4)

    101

    98-104

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    40

    2.283

    2.334

    2.309

    2.158

    20

    1.562

    1.667

    1.615

    1.464

    10

    1.020

    1.028

    1.024

    0.873

    5

    0.644

    0.629

    0.637

    0.486

    2.5

    0.402

    0.391

    0.397

    0.246

    1.25

    0.288

    0.273

    0.280

    0.129

    0.625

    0.206

    0.194

    0.200

    0.049

    0

    0.147

    0.155

    0.151

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CC16 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled CC16 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
    CC16是一種肺泡表面活性蛋白，具有免疫抑制和抗炎作用，可用于肺疾病的診斷和監(jiān)測。最新研究發(fā)現(xiàn)，CC16水平與慢性阻塞性肺疾病、哮喘、肺部感染等多種呼吸系統(tǒng)疾病相關，并且可能參與心血管疾病和神經(jīng)系統(tǒng)疾病的發(fā)生發(fā)展。
  • 功能：
    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • 基因功能參考文獻：
    1. There was no evidence that serum CC16 levels played a role in recurrent wheezing and a positive asthma predictive index in pre-school children PMID: 29685782
    2. Data suggest that the increase in plasma club cell protein (CC16) CC16 following inhaled allergen as a biomarker of epithelial dysfunction. PMID: 28862880
    3. We propose that increased CC-16 levels is a marker of lung inflammatory response that associated with ventilatory insufficiency are related to impending respiratory failure, not fully predicted by conventional respiratory tests PMID: 28967121
    4. Studied serum CC16 levels in The serum CC16 levels in Acute respiratory distress syndrome (ARDS) and non-ARDS patients; found CC16 to be were significantly higher in ARDS patients than that in non-ARDS patients. PMID: 28548310
    5. Studied serum CC16 levels in relation to atopy and previously manifested low respiratory tract diseases. Found low serum CC16 is associated with previously expressed pneumonia and chronic wheezing in atopic children. PMID: 28146340
    6. Our data suggest that rCC16 suppresses LPS-mediated inflammatory mediator TNF-alpha, IL-6, and IL-8 production by inactivating NF-kappaB and p38 MAPK but not AP-1 in RAW264.7 cells. PMID: 28338974
    7. There were associations between urinary CC16 and arsenic concentration in soil, water, house dust, and dust loading. In multiple analyses, only the concentration of arsenic in soil was associated with urinary CC16 levels after accounting for other factors. PMID: 27223295
    8. findings demonstrate that CC16 is upregulated in IPF patients suggesting that may participate in its pathogenesis. PMID: 27977812
    9. The G38A CCSP gene polymorphism may alter either the production of the protein and/or its activity in chronic obstructive pulmonary disease. PMID: 27496897
    10. CC16 levels differed among idiopathic pulmonary fibrosis, pulmonary sarcoidosis and chronic pulmonary obstructive disease. PMID: 27758987
    11. Lung-specific (CC-16) and novel (RelB) biomarkers are associated with systemic cardiovascular changes over time. CC-16 can predict subsequent exacerbations in subjects with severe COPD and may be an important biomarker of pulmonary and systemic stress in COPD. PMID: 26914709
    12. The serum concentration of CC16 was significantly higher in patients with lung fibrosis. PMID: 25244495
    13. lower levels of urine CC16 and lung function in patients with asthma than in those patients without asthma. CC16 in urine may be a useful tool or biomarker for investigating lung epithelium integrity among children with asthma or lung injury. PMID: 26108072
    14. Smokers and COPD patients had reduced airway CC16 immunostaining that decreased with increasing COPD severity. PMID: 25700379
    15. the uteroglobin G38A gene polymorphism was not associated with IgAN risk PMID: 25068828
    16. Sputum and bronchoalveolar lavage fluid CC16 were significantly higher in patients with severe asthma compared to mild-moderate asthma and healthy controls. PMID: 25728058
    17. In chromium-exposed workers, blood levels of CC16, and CC16/SP-D were lower than in controls. Positive relationships were shown between CC16 or CC16/SP-D and indicators of lung function. PMID: 25851191
    18. KL-6 levels were higher and CC16 levels were lower in infants with poor neurodevelopmental outcome compared with those infants who had favourable neurodevelopmental outcome. PMID: 25631862
    19. CC16 may play an important protective role in cigarette smoke-related diseases. PMID: 25635997
    20. A meta-analysis indicated that the CC16 gene A38G polymorphism is not associated with the risk of asthma. PMID: 25743006
    21. Increased plasma clara cell secretory protein levels are associated with primary graft dysfunction. PMID: 24400993
    22. Uteroglobin is a possible ligand of the lipoxin receptor and it may have a role in inhibiting serum amyloid A-driven inflammation PMID: 24782597
    23. Athletes with decreased serum CC16 from regular high-load exercise are more susceptible to respiratory infections. PMID: 24735334
    24. A single therapeutic dose of terbutaline offers significant protection against hyperpnoea-induced bronchoconstriction and reduces urinary CC16 protein levels. PMID: 24030662
    25. Lung permeability biomakers [surfactant protein D (SP-D) and Clara cell secretory protein (CC16) in plasma] and forced expiratory volumes and flow were measured in swimmers in indoor swimming pool waters treated with different disinfection methods. PMID: 23874631
    26. Serum CC-16 is associated with disease progression in chronic obstructive pulmonary disease (COPD). However, the absence of CC-16 does not appear to modify the risk of cigarette-related COPD in mice. PMID: 24245748
    27. Acute exposure to smoke induces injury at the alveolar level, which results in a transient increase of CC16 in serum of exposed subjects. PMID: 23258467
    28. These results suggest that the CC16 A38G polymorphism may play a role in asymptomatic airway hyper-responsiveness and contribute to the development of late-onset asthma. PMID: 24125144
    29. The SCGB1A1 +38A/G polymorphism is a risk factor for asthma. [Meta-analysis] PMID: 23820082
    30. urinary CC16 may be a useful biomarker of increased lung epithelial permeability among female non-smokers; further work will be required to evaluate its applicability to males PMID: 22805990
    31. Clara cell secretory protein expression changes is a relevant marker in the development of bronchiolitis obliterans patients with lung transplantation. PMID: 22883104
    32. Overview of CC16 in pathophysiology of and as a biomarker in chronic obstructive pulmonary disease. PMID: 23030587
    33. A Genome wide association study for COPD biomarkers on subjects with COPD found 2 discrete loci affecting CC16, one near the CC16 coding gene (SCGB1A1) on chromosome 11 and one approximately 25Mb away from SCGB1A1, identified in expressed sputum. PMID: 23144326
    34. Donor CCSP A38G polymorphism is associated with decreased CCSP levels early after lung transplantation and poor long-term outcomes. PMID: 22902791
    35. Exercise caused an increase in urinary excretion of CC16 in all subjects (P < 0.001), but this rise in CC16 was blunted following inhalation of warm humid air. PMID: 21799131
    36. demonstration that cc-10 is differentially expressed in infants with iRDS may point the way towards one possible mechanism that potentially involves modifications of the protein structure with its anti-inflammatory and surfactant protective function PMID: 22613976
    37. CC10 G+38A variant may contribute to the severity of asthma and lower level of steroid responsiveness. PMID: 22788242
    38. These results indicate that CC10 gene transfer may inhibit airway inflammation through suppressing the activation of NF-kappaB. PMID: 22558282
    39. Serum Clara cell secretory protein levels were characterized by an early postnatal surge. This apparent gestation-influenced surge may represent an initiation of a protective cascade against postnatal lung injury during extrauterine adaptation. PMID: 21952535
    40. Single-nucleotide polymorphism in the CC10 gene (A38G) does not seem to be involved in the severity of respiratory syncytial virus infection or wheezing. PMID: 21767304
    41. Urinary levels of CC16 are increased after eucapnic voluntary hyperpnea. PMID: 21131866
    42. Elevated circulating CC16 levels identified severe thoracic injury combined with a strong correlation with the extent/volume of affected lung tissue. PMID: 21045740
    43. CC16 38A/38A genotype plays a role in the development of early asthma in children with AR. PMID: 21255142
    44. Elevated plasma clara cell secretory protein concentration is associated with high-grade primary graft dysfunction. PMID: 21299834
    45. In the context of allergic airway responses, CC10 can inhibit OPN expression and suppress the Th2-promoting function of OPN, resulting in CC10's inhibitory biological effects. PMID: 20553297
    46. Data suggest that a supernatant of non-small-cell lung cancer causes an imbalance in the immune response of PBMCs and DCs, which is reversed by CC-10. PMID: 20664959
    47. Letter: serum CCSP cannot be used as a biomarker predictive for bronchiolitis obliterans after lung transplantation. PMID: 20683434
    48. Reduced anti-inflammatory CC10 concentrations in airways of extremely premature infants with a fetal inflammatory response, not umbilical cord serum CC10, might make their lungs susceptible for further postnatal injuries. PMID: 19887851
    49. Association of CC16 with daily outdoor particulate matter from combustion sources increases epithelial barrier permeability in lungs. PMID: 19852548
    50. The effect of G38A may be apparent under stimulation as sex steroids or infections, and homozygotes of the G38A mutation cannot produce sufficient UG in response to stimulation and may be predisposed to IgA nephropathy, especially in childhood. PMID: 11774099

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Secretoglobin family
  • 組織特異性：
    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12523

    OMIM: 192020

    KEGG: hsa:7356

    STRING: 9606.ENSP00000278282

    UniGene: Hs.523732