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  4. Human Claudin-2(CLDN2) ELISA kit

Human Claudin-2(CLDN2) ELISA kit

  • 中文名稱：
    人封閉蛋白2(CLDN2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL005500HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人封閉蛋白2(CLDN2)酶聯(lián)免疫試劑盒（CSB-EL005500HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CLDN2含量。CLDN2 即閉合蛋白 2，是緊密連接蛋白家族成員。它在維持細胞極性及屏障功能等方面有重要作用。研究發(fā)現(xiàn)其在多種腫瘤中異常表達，可能參與腫瘤細胞增殖、遷移等過程，有望成為腫瘤診斷和治療的新靶點。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，該產品廣泛應用于細胞生物學、病理機制及藥物干預等基礎研究，例如探究上皮屏障功能障礙模型、炎癥性疾病動物實驗或腫瘤細胞間連接相關分子機制，為科研人員提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    CLDN2; PSEC0059; SP82; UNQ705/PRO1356; Claudin-2; SP82
  • 縮寫：
    CLDN2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.86 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %80-92
    1:2Average %90
    Range %85-95
    1:4Average %97
    Range %92-103
    1:8Average %93
    Range %88-98
  • 回收率：
    The recovery of human CLDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104100-109
    EDTA plasma (n=4)9690-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.671 2.552 2.612 2.449
    7502.124 2.006 2.065 1.902
    3751.438 1.403 1.421 1.258
    187.50.806 0.839 0.823 0.660
    940.508 0.524 0.516 0.353
    470.294 0.309 0.302 0.139
    23.50.234 0.227 0.231 0.068
    00.161 0.164 0.163
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 基因功能參考文獻：
    1. CLDN2 may have a role regulating tissue-specific physiological properties in tight junctions in UC. PMID: 29040430
    2. the accumulation of DXR into spheroids was enhanced by claudin-2 knockdown, resulting in a reduction in cell viability. We suggest that claudin-2 may be a novel therapeutic target in lung adenocarcinoma, because claudin-2 knockdown increased the accumulation of anticancer agents in cancer cells and spheroids. PMID: 29247669
    3. integration of claudin-2, occludin and ZO-1 is necessary for maintaining the function of the proximal tubular epithelium. PMID: 29252987
    4. these findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2 PMID: 28893958
    5. Low CLDN2 expression is associated with Lung Cancer metastasis. PMID: 28754672
    6. results reveal that IL-22 increases intestinal epithelial permeability by upregulating Claudin-2 expression through the JAK/STAT pathway PMID: 28939759
    7. In summary, we demonstrate that TCPTP protects the intestinal epithelial barrier by restricting STAT-induced claudin-2 expression. PMID: 28804910
    8. The cation channel-forming tight junction protein claudin-2 was shown to permit the paracellular passage of water through its pore. PMID: 28636801
    9. suggest that epigenetic inhibitors suppress the abnormal proliferation of lung adenocarcinoma cells highly expressing claudin-2 PMID: 28057758
    10. Data show that alterations in myosin light chain kinase activity, claudin-15 and claudin-2 expression are associated with gluten-induced symptomatology and intestinal permeability changes in diarrhea-predominant irritable bowel syndrome (IBS-D). PMID: 27869798
    11. hypotonic stress induces dephosphorylation, clathrin-dependent endocytosis, and degradation of claudin-1 and -2 in lysosomes, resulting in disruption of the TJ barrier in renal tubular epithelial cells. PMID: 27733684
    12. The claudin-2 expression increased in tumor necrosis factor alpha (TNF-alpha) Treated Caco-2 cell monolayers. PMID: 28106723
    13. The authors conclude that claudin-2 forms gated paracellular channels. PMID: 26568313
    14. Association of claudin2 (rs7057398) polymorphism with idiopathic recurrent acute pancreatitis and progression of the disease to chronic pancreatitis. PMID: 26110235
    15. Genetic variants in CLDN2 and MORC4 genes were associated with chronic pancreatitis in Indian patients. PMID: 26820620
    16. Quercetin decreases claudin-2 expression mediated by up-regulation of miR-16 expression and instability of claudin-2 mRNA in lung adenocarcinoma cells. PMID: 26061016
    17. Pan-inhibition of SFK signaling pathways elevated Claudin-2 expression levels in breast cancer cells. Loss of Yes or Fyn induces Claudin-2 expression; whereas, diminished Lyn levels impairs Claudin-2 expression in breast cancer cells. PMID: 25823815
    18. PKCiota binds to Rab14 and that PKCiota requires Rab14 for its correct distribution in cells. As with Rab14, PKCiota protects claudin-2 from lysosomal degradation and, in consequence, modulates epithelial barrier. PMID: 25694446
    19. Phosphorylation state of the tyrosine of claudin-1 and claudin-2 regulates interaction with ZO1. PMID: 26023235
    20. Single nucleotide polymorphism is associated with the development of alcoholic pancreatitis. PMID: 25253127
    21. TNF--alpha modifies claudin-2 expression in tubular cells and this can have both short-term and long-term effects on transport and barrier functions. PMID: 25948735
    22. Claudin-5 overexpression promoted the interactions of claudin-1 and claudin-2 and enhanced the barrier function of retinal cells. PMID: 25323998
    23. Claudins 2 and 4 have reciprocal effects on epithelial barrier function, exhibit differential FRAP dynamics, and compete for residency within the tight junction. PMID: 25031428
    24. Autophagy reduces the paracellular permeability of small solutes and ions via degradation of the pore-forming tight junction protein claudin-2. PMID: 25616664
    25. Decreased claudin-2 enhances endothelial barrier function and may play a role in the prolonged survival of patients with Kaposi sarcoma-associated herpesvirus and HIV co-infection. PMID: 24995964
    26. IL-6 modulation of intestinal TJ permeability was regulated by JNK activation of AP-1 and AP-1 activation of claudin-2 gene PMID: 24662742
    27. Suggest that claudin 2 is involved in paracellular water transport and that claudin 2 expression is sensitive to compound induced cellular stress. PMID: 24907557
    28. The expression of claudin-2 was down regulated in gastric cancer tissue. PMID: 23919729
    29. In breast cancer patients CLDN2 was significantly up-regulated (P < 0.001) in liver metastases compared to other metastatic sites. PMID: 24287398
    30. Proliferation of human adenocarcinoma A549 cells was decreased by claudin-2 knockdown together with a decrease in the percentage of S phase cells. PMID: 24907662
    31. Report altered claudin 1 expression when comparing normal skin to actinic keratosis and squamous cell carcinoma of the skin. PMID: 24294371
    32. Helicobacter pylori-induced tumorigenesis and invasiveness of gastric carcinoma cells is mediated by bacterial CagA via Cdx2-dependent targeting of Claudin-2. PMID: 24287273
    33. Findings suggest that a variant of the CLDN2-MORC4 region predisposes to Crohn's disease in a Swedish population. PMID: 23946598
    34. Claudin-2 protein expression is elevated in intestinal epithelia, in response to bovine colostrum, without altering ion permeability. PMID: 23717570
    35. generated MDCK I Tet-off cells stably transfected with claudin-2 Tyr(67) mutants. The Y67L mutant showed reduced cation selectivity compared with wild-type claudin-2 due to a decrease in Na(+) permeability, without affecting the Cl(-) permeability PMID: 23760508
    36. Claudin-2 assumes an important role in colorectal inflammation, and furthermore implicates the involvement of MLCK in colon inflammation. PMID: 23306855
    37. Sinonasal epithelium in allergic fungal rhinosinusitis displays increased epithelial permeability and an altered expression of claudin-2 PMID: 22927233
    38. results implicate pericryptal myofibroblast-derived paracrine KGF and largely autocrine amphiregulin in the upregulation of claudin-2 in Caco-2 epithelial monolayers and consequent disruption of tight junction integrity PMID: 22946653
    39. Two associations with alcoholic pancreatitis at genome-wide significance were identified and replicated at PRSS1-PRSS2 and X-linked CLDN2 through a two-stage genome-wide study. PMID: 23143602
    40. These results suggest that EGF is secreted from A549 cells by MMP and increases claudin-2 expression mediated via the activation of an EGFR/MEK/ERK pathway. PMID: 22546605
    41. Pore-forming tight junction protein claudin-2 is strongly expressed in the ileum of 51% patients in quiescent phase and in 49% of the patients with active Crohn's disease. PMID: 21688348
    42. demonstrate here that breast cancer cells harbouring PIK3CA mutations are selectively sensitive to mTOR allosteric and kinase inhibitors. However, cells with PTEN loss of function are not sensitive to these drugs PMID: 21383692
    43. CLDN2 is selectively enriched in and promotes the formation of breast cancer liver metastases through engagement of cell membrane complexes. PMID: 21076473
    44. The authors propose claudin-2 and SYN1 work in concert to enhance microbial translocation across the intestinal epithelial barrier to contribute to chronic immune activation and CD4 T-cell depletion in HIV-1-infected patients. PMID: 20700059
    45. claudin-2 was not expressed in any of the 18 cases of Epstein-Barr virus-associated nasopharyngeal carcinoma studied. PMID: 20204275
    46. CLDN 2 expression was significantly increased in severe form of coeliac disease in bulb and in distal duodenum PMID: 20143085
    47. Cloning of the human claudin-2 5'-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1alpha. PMID: 11934881
    48. Results support a model in which claudins 2 and 4 create paracellular channels and the first extracellular domain is sufficient to determine both paracellular charge selectivity and transepithelial electrical resistance. PMID: 12700140
    49. role for the paracellular barrier function by opening pores for small cations PMID: 14751232
    50. optimal claudin-2 expression in the gut relies on the presence of GATA-4, suggesting a role for this factor in intestinal regionalization PMID: 15389642

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  • 亞細胞定位：
    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Claudin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2041

    OMIM: 300520

    KEGG: hsa:9075

    STRING: 9606.ENSP00000336571

    UniGene: Hs.522746