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中文名稱:人凝血因子XIII A1 (F13A1)酶聯(lián)免疫試劑盒
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貨號:CSB-E14291h
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人凝血因子XIII A1 (F13A1)酶聯(lián)免疫試劑盒(CSB-E14291h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的F13A1含量。人凝血因子XIII A1(F13A1)是凝血級聯(lián)反應(yīng)中最后一個被激活的酶原,由兩個A亞基和兩個B亞基組成的異四聚體。A亞基具有催化功能,參與纖維蛋白交聯(lián)和穩(wěn)定,而B亞基作為載體保護(hù)A亞基。F13A1缺陷會導(dǎo)致出血傾向、傷口愈合缺陷和習(xí)慣性流產(chǎn)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,適用于基礎(chǔ)醫(yī)學(xué)研究中分析F13A1在凝血機(jī)制中的作用、評估疾病模型(如血栓形成或出血性疾?。┲性撘蜃拥谋磉_(dá)變化,或用于藥物開發(fā)中篩選調(diào)控凝血功能的化合物,為探索凝血相關(guān)分子機(jī)制提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:bA525O21.1 (coagulation factor XIII; A1 polypeptide) ELISA Kit; Coagulation factor XIII A chain ELISA Kit; Coagulation factor XIII A1 polypeptide ELISA Kit; Coagulation factor XIII A1 subunit ELISA Kit; Coagulation factor XIII; A polypeptide ELISA Kit; Coagulation factor XIIIa ELISA Kit; F13A ELISA Kit; F13A_HUMAN ELISA Kit; F13a1 ELISA Kit; Factor XIIIA ELISA Kit; Fibrin stabilizing factor; A subunit ELISA Kit; Fibrinoligase ELISA Kit; FSF; A subunit ELISA Kit; Protein glutamine gamma glutamyltransferase A chain ELISA Kit; Protein-glutamine gamma-glutamyltransferase A chain ELISA Kit; TGase ELISA Kit; Transglutaminase A chain ELISA Kit; Transglutaminase; plasma ELISA Kit; Transglutaminase. plasma ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma
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檢測范圍:0.625 ng/mL-40 ng/mL
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靈敏度:0.156 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Blood Coagulation
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human F13A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 85 Range % 81-89 1:2000 Average % 93 Range % 90-96 1:4000 Average % 87 Range % 83-90 1:8000 Average % 100 Range % 97-103 -
回收率:
The recovery of human F13A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 84 80-88 EDTA plasma (n=4) 100 95-105 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 40 2.783 2.798 2.791 2.622 20 2.324 2.368 2.346 2.177 10 1.768 1.760 1.764 1.595 5 1.153 1.168 1.161 0.992 2.5 0.691 0.672 0.682 0.513 1.25 0.401 0.406 0.404 0.235 0.625 0.295 0.276 0.286 0.117 0 0.170 0.168 0.169 -
數(shù)據(jù)處理:
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貨期:3-5 working days
產(chǎn)品評價
樣品類型:其他樣品處理方法 滅活
樣品信息:人
稀釋比:沒有稀釋
產(chǎn)品評價: 我用CSB-E14291h試劑盒檢測胸水上清。靈敏度高,能夠檢測到較低濃度的人凝血因子XIII A1 (F13A1),對于一些含量較低的樣本也能進(jìn)行準(zhǔn)確的定量分析。特異性好:具有高特異性,與其他相關(guān)蛋白無顯著交叉反應(yīng),這意味著在檢測過程中,能夠準(zhǔn)確地識別和測量目標(biāo)蛋白F13A1,不會受到其他類似蛋白的干擾,從而保證了實驗結(jié)果的準(zhǔn)確性和可靠性,使研究者可以更有信心地解讀實驗數(shù)據(jù)。精密度高,這表明試劑盒在同一批次和不同批次之間的實驗結(jié)果具有很好的一致性,減少了實驗誤差,提高了實驗的重復(fù)性和可比性,對于需要進(jìn)行大量樣本檢測或長期研究的項目來說,能夠確保數(shù)據(jù)的穩(wěn)定性和可信度
By 張老師
相關(guān)產(chǎn)品
靶點詳情
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功能:Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
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基因功能參考文獻(xiàn):
- Overall, genetically-determined FXIIIA levels have a significant long-term prognostic role, suggesting that a pharmacogenetics approach might help to select those AMI patients at risk of poor prognosis in the need of dedicated treatments. PMID: 30223472
- Inflammatory monocytes highly express Factor XIIIA, which promotes fibrin cross-linking to create a scaffold for lung squamous carcinomas cell invasion and metastases. PMID: 29777108
- Effect of factor XIII levels and polymorphisms on the risk of myocardial infarction in young patients PMID: 29484525
- Coagulation factor FXIII-A (FXIIIA) was found to express specifically in the fetal beta islets but not in the alpha/delta islets. PMID: 29424810
- Factor XIIIa (AC-1A1) is a sensitive and specific nuclear marker for sebaceous differentiation...which can be utilized to aid in the diagnosis of sebaceous neoplasms. PMID: 28873247
- Our meta-analysis supports an association between F13A1 Val34Leu and recurrent pregnancy loss. PMID: 28683377
- A brief history of some key events in the conversion of fibrinogen to fibrin has been reviewed. (Review) PMID: 27519977
- The results of this study suggest that these biomarker F13A1 can serve as a potential non-invasive early diagnosis platform reflecting PiB-PET imaging for Mild Cognitive Impairment and Alzheimer's Disease. PMID: 27392853
- F13A1 gene mutations in 73 patients treated with recombinant FXIII-A2 PMID: 28520207
- Results show that the missense mutations-causing mild FXIII de fi ciency influence different aspects of FXIII function and can be functionally categorized on the basis of their expression phenotype. PMID: 27363989
- These findings provide insight into assembly of the fibrinogen/FXIII-A2B2 complex in both physiologic and therapeutic situations. PMID: 27561317
- FXIIIa exhibits a preference for Q237 in crosslinking reactions within fibrinogen alphaC (233-425) followed by Q328 and Q366. PMID: 26951791
- factor XIII Val34Leu polymorphism is associated with coronary artery diseases risk, especially myocardial infarction. Age and sex did not affect the relationship between factor XIII Val34Leu polymorphism and diseases risk (Meta-Analysis) PMID: 27665853
- Mounting evidence now suggests that platelet FXIII-A modulates hemostasis by several different mechanisms. This condensed review discusses recent advances in the understanding of the novel intracellular and extracellular functions of platelet FXIII-A. [review] PMID: 27207415
- It is a unique case showing the combination of a highly aggressive angiosarcoma and presence of inherited FXIII deficiency. It is also a rare example demonstrating the benefit of FXIII genotyping besides the expected acquired FXIII deficiency possibly due to neoplasm induced increased consumption by elevated crosslinking of fibrin fibers. PMID: 26540128
- The Val34Leu polymorphism of FXIII was not found in Korean people, and compared with Caucasians, a noticeably low incidence of deep vein thrombosis was shown. PMID: 26802299
- Genetic basis of severe factor XIII deficiency in a large cohort of Indian patients has been uncovered. PMID: 26852661
- Our meta-analysis suggests that there is no evidence for strong association between FXIII Val34Leu polymorphisms and intracerebral hemorrhage--{review} PMID: 26121426
- Deletion of 11 or more N-terminal amino acids disrupts intersubunit interactions, which may prevent FXIII-A2 homodimer formation. AP-FXIII plays an important role in the stability of the FXIII-A2 dimer. PMID: 26083359
- Mutations in the activation peptide of full-length recombinant FXIII regulate activation rates by thrombin, and V34L influences in vivo thrombus formation by increased cross-linking of the clot. PMID: 26743168
- Different FXIII-A dynamics and levels could be utilised as early prognostic indicators during acute MI, revealing the individual potential to heal and suggesting tailored treatments to avoid heart failure or its extreme consequence. PMID: 25947356
- FXIII Val34Leu polymorphism has a protective effect against recurrent spontaneous abortion. PMID: 25862345
- Report immunochromatographic test for detection of anti-factor XIII A subunit antibodies that can diagnose 90 % of cases with autoimmune haemorrhaphilia XIII. PMID: 25740658
- These results indicate that FXIIIa activity can be modulated by fibrinolytic enzymes, and suggest that changes in fibrinolytic activity may influence cross-linking of blood proteins. PMID: 26359437
- Suggest that FXIIIa positive dermal dendrocytes may be the main antigen presenting cells in indeterminate leprosy. PMID: 25365500
- there is an evidence for association between factor XIII Val34Leu polymorphism and CSX PMID: 23677728
- These findings expose a newly recognized, essential role for fibrin crosslinking during whole blood clot formation and consolidation and establish FXIIIa activity as a key determinant of thrombus composition and size. PMID: 26324704
- Results show that FXIII-B Arg95 variant is associated with an increased risk of abdominal aortic aneurysms (AAA) suggesting a possible role for FXIII in AAA pathogenesis. PMID: 25384012
- The results demonstrate that FXIII-A Val34Leu polymorphism does not influence the occurrence of atherothrombotic ischemic stroke, but has an effect on the severity of its outcome. PMID: 24686102
- His343Gln was a novel missense mutation occurring in the core domain of the FXIII A subunit. This is the first report of genetically confirmed FXIII deficiency in Korea, with novel and recurrent F13A1 mutations. PMID: 25004025
- study suggested that FXIIIA Val34Leu polymorphism was a protective factor for myocardial infarction in caucasians PMID: 24042156
- Genotype 163TT of FXIII-A gene as a new independent risk factor for the development of Venous Thromboembolism in young women living in the North-West region of Russia. PMID: 26035561
- This study presents the covalent structure of single-stranded fibrin oligomers cross-linked by FXIIIa. PMID: 25896761
- a cohort of 27 individuals was analysed and four novel mutations leading to congenital FXIII deficiency were reported PMID: 24329762
- FXIII-A has a functional role through exposure on the activated platelet membrane where it exerts antifibrinolytic function by cross-linking alpha2AP to fibrin PMID: 25331118
- 8 new heterozygous missense mutations (Pro166Leu, Arg171Gln, His342Tyr, Gln415Arg, Leu529Pro, Gln601Lys, Arg703Gln and Arg715Gly) may affect catalysis, barrel domain integrity or activation peptide cleavage, depending on domain. PMID: 24889649
- results demonstrate the utility of eQTL mapping in the identification of novel asthma genes and provide evidence for the importance of FADS2, NAGA, and F13A1 in the pathogenesis of asthma. PMID: 24934276
- FXIII Val34Leu and PAI-1 4G/5G polymorphisms are prevalent in Egyptian women, with unexplained primary first trimester Recurrent miscarriage and combined polymorphisms statistically increasing the risk. PMID: 24702949
- FXIII-A serves as a preadipocyte-bound proliferation/differentiation switch that mediates effects of hepatocyte-produced circulating pFN. PMID: 24934257
- plasma FN assembly into bone matrix in vitro requires FXIIIA transglutaminase activity making pFN assembly an active, osteoblast-mediated process. PMID: 24246248
- this study presents for the first time an in depth and time-resolved analysis of the FXIIIa substrate proteome in plasma. PMID: 24443567
- Patients with advanced-stage NSCLC exhibited higher coagulation FXIII activity than healthy controls and early-stage NSCLC patients. PMID: 24142643
- These data suggest that plasma transglutaminase factor XIII may play a key role in fetal development of vertebrates via cross-link of Fas antigen. PMID: 24216108
- Data suggest that Factor XIII (composed of subunits F13A and F13B) increases rigidity/strength of fibrin clot, protects fibrin clot against shear stress in circulation, and protects fibrin from prompt elimination by fibrinolytic system. [REVIEW] PMID: 24476525
- Studies indicate that mutations in the factor XIII-A (FXIII-A) gene causing congenital factor XIII deficiency. PMID: 23929307
- Overproduction of FXIII-A by M2 macrophages might contribute to the excessive fibrin deposition in the submucosa of nasal polyps (NP), which might contribute to the tissue remodeling and pathogenesis of chronic rhinosinusitis with NP. PMID: 23541322
- The Fctor XIIIa R260C mutant has significantly altered conformations, resulting in the rapid degradation by the proteasome inside the synthesizing cells PMID: 23279035
- No association has been found between FXIII-A Val34Leu genotype and the risk for peripheral arterial disease in a Hungarian cohort of patients. PMID: 23518792
- The prevalence of a F13A1 gene intron 1 variant (IVS1+12C>A) was found with greater frequency in patients with a mild FXIII deficiency with no detectable F13A1 or F13 B mutations than in those with heterozygous F13A1 mutations or in normal controls. PMID: 23508224
- The results suggest an increase in FXIII activity as the number of repetitions of the short tandem repeat polymorphism in the F13A01 gene increased up to allele 5. PMID: 22909824
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相關(guān)疾?。?/div>Factor XIII subunit A deficiency (FA13AD)亞細(xì)胞定位:Cytoplasm. Secreted. Note=Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.蛋白家族:Transglutaminase superfamily, Transglutaminase family數(shù)據(jù)庫鏈接:
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