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  4. Human Coenzyme Q10 (CoQ10) ELISA Kit

Human Coenzyme Q10 (CoQ10) ELISA Kit

  • 中文名稱：
    人輔酶Q10 (CoQ10)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14081h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人輔酶Q10 (CoQ10)酶聯(lián)免疫試劑盒（CSB-E14081h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CoQ10含量。CoQ10即輔酶Q10，是一種存在于人體細(xì)胞內(nèi)的脂溶性醌類化合物，參與細(xì)胞能量生成與抗氧化防御。研究機(jī)制方面，它在線粒體呼吸鏈中傳遞電子、質(zhì)子，促進(jìn)ATP合成，還能清除自由基，保護(hù)細(xì)胞免受氧化損傷。試劑盒檢測(cè)范圍為3.12 ng/mL-50 ng/mL，支持能量代謝研究、疾病模型機(jī)制探索（如心肌缺血、帕金森病等）以及膳食補(bǔ)充劑效果評(píng)估等實(shí)驗(yàn)需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    CoQ10
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 ng/mL-50 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 96  
    Range % 91-101  
    1:40 Average % 99  
    Range % 93-104  
    1:80 Average % 92  
    Range % 87-96  
    1:160 Average % 90  
    Range % 85-94  
  • 回收率：
    The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-88  
    EDTA plasma (n=4) 93 88-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.112 0.103 0.108    
    25 0.208 0.224 0.216    
    12.5 0.377 0.397 0.387    
    6.25 0.783 0.718 0.751    
    3.12 1.121 1.086 1.104    
    0 1.951 1.829 1.890    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

Please provide the following information for Human Coenzyme Q10, CoQ10 ELISA Kit (cat:CSB-E14081h):
1.What is the standard?
2.What is the antibody in this kit?
3. Is the antibody against lipid Coq10 or to its binding protein?
4. will binding protein be labeled to Co1q10 or directly coq10?

A:
Thanks for your inquiry.
The standard is purified CoQ10.
This kit detects total CoQ10, and the antibody in the kit is mouse monoclonal antibody.
The antibody is specific to CoQ10.Pls let me know if you have any further questions. Thank you.