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  4. Human Collagen alpha-1(X) chain (COL10A1) ELISA kit

Human Collagen alpha-1(X) chain (COL10A1) ELISA kit

  • 中文名稱：
    人X型膠原α-1鏈(COL10A1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL005715HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人X型膠原α-1鏈(COL10A1)酶聯(lián)免疫試劑盒（CSB-EL005715HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的COL10A1含量。人X型膠原α-1鏈（COL10A1）是一種膠原蛋白，主要研究其在骨關(guān)節(jié)疾病中的作用。近年來，COL10A1在腫瘤中的作用受到關(guān)注，其可能通過改變腫瘤微環(huán)境成分，促進腫瘤發(fā)生和轉(zhuǎn)移，具有成為多腫瘤治療靶點的潛力。研究機制涉及COL10A1與腫瘤免疫微環(huán)境的關(guān)系，以及其在腫瘤細胞增殖、遷移和侵襲中的作用。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于科研領(lǐng)域探索骨骼生長、軟骨分化、骨關(guān)節(jié)疾病模型中的分子機制，或用于體外培養(yǎng)細胞中COL10A1表達水平的定量分析，為骨代謝相關(guān)基礎(chǔ)研究及藥物開發(fā)提供可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    COAA1_HUMAN ELISA Kit; Col10a 1 ELISA Kit; COL10A1 ELISA Kit; Collagen alpha 1(X) chain ELISA Kit; Collagen alpha-1(X) chain ELISA Kit; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) ELISA Kit; Collagen type X alpha 1 ELISA Kit; Collagen X alpha 1 polypeptide ELISA Kit; CollagenX ELISA Kit; fa66d11 ELISA Kit; fb10c08 ELISA Kit; OTTHUMP00000040411 ELISA Kit; Procollagen type X alpha 1 ELISA Kit; Schmid metaphyseal chondrodysplasia ELISA Kit; wu:fa66d11 ELISA Kit; wu:fb10c08 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human COL10A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-106
    1:2Average %92
    Range %88-95
    1:4Average %95
    Range %90-101
    1:8Average %96
    Range %91-103
  • 回收率：
    The recovery of human COL10A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-101
    EDTA plasma (n=4)9287-99
  • 標(biāo)準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    203.039 3.052 3.046 2.909
    102.647 2.658 2.653 2.516
    51.898 2.040 1.969 1.832
    2.51.211 1.262 1.237 1.100
    1.250.708 0.663 0.686 0.549
    0.6250.425 0.406 0.416 0.279
    0.3120.242 0.257 0.250 0.113
    00.138 0.136 0.137 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • 基因功能參考文獻：
    1. Plasma samples from lung cancer patients and healthy heavy-smokers controls were tested for levels of COL11A1 and COL10A1 (n = 57 each) and SPARC (n = 90 each). Higher plasma levels of COL10A1 were detected in patients (p PMID: 30227835
    2. All affected individuals are heterozygous for the missense mutation collagen type X alpha 1 chain (COL10A1) rs111033552. PMID: 29234170
    3. increased expression of stromal colXalpha1 and low TILs correlate with poor pathologic response in ER+/HER2+ breast tumors. Further studies are needed to confirm their predictive value and impact on long-term outcomes, and to determine whether this collagen exerts a protective effect on the cancer cells or simply reflects other factors within the tumor microenvironment PMID: 27090210
    4. a novel sequence variation involving an unusual mutational site of the COL10A1 gene can cause mild metaphyseal chondrodysplasia PMID: 25542771
    5. COL10A1 mutation 2005delC in a Chinese pedigree with Schmid type metaphyseal chondrodysplasia is close to the C-terminus of the protein sequence and may result in genetic heterogeneity of the Chinese population PMID: 25974987
    6. Concentration of serum collagen type X levels correlated with cartilage degradation in osteoarthritis patients. PMID: 25245039
    7. The results show that COL10A1 is a tumor biomarker upregulated in a wide variety of tumors including those of the breast, colon, bladder, stomach, esophagus, lung, testis, ovary and pancreas. PMID: 22894674
    8. Yiqi Huayu Bushen Recipe increased the expression of aggrecan, decreased the expression of type X collagen, and promoted cell proliferation in cells from degenerated human intervertebral discs. PMID: 22015197
    9. Genetic variation near FRK/COL10A1 and VEGFA are associated with advanced age-related macular degeneration. PMID: 21665990
    10. MCDS is a rare genetic skeletal disorder caused by a collagen type X defect. Though much is known about the molecular pathology of the causative COL10A1 mutations, causal therapy of the disease is not yet available PMID: 21360259
    11. a frameshift mutation leading to elongation of the deduced alpha1(X) chain associated with Metaphyseal Chondrodysplasia type Schmid PMID: 21447328
    12. These results indicate that nitrogen-rich plasma polymerized surfaces inhibit COL10A1 expression via the suppression of COX-1. PMID: 20225218
    13. speculate that complete loss of mutant transcripts yields COL10A1 haploinsufficiency and late clinical presentation while incomplete loss of mutant transcripts yields dominant-negative effects with early clinical presentation PMID: 20872587
    14. The total expression of type X collagen in the concave side growth plates of the lower end vertebrae was higher than that in the same side growth plates of apex. PMID: 20073986
    15. methylation-based COL10A1 gene silencing is established in cartilage tissue and human articular chondrocytes during chondrogenesis. PMID: 18759285
    16. chains harboring Schmid metaphyseal chondrodysplasia NC1 domain mutations are selectively retained and degraded in stably transfected cells PMID: 11805116
    17. The crystal structure at 2.0 A resolution of the human collagen X NC1 domain reveals an intimate trimeric assembly strengthened by a buried cluster of calcium ions. PMID: 11839302
    18. The 4.6 kb promoter is able to drive specific expression of Col10a1 in hypertrophic cartilage. PMID: 15464363
    19. exposure of the NC1 thiol may trigger the recognition and degradation of mutant collagen X chains PMID: 15695517
    20. The effect of COL10A1 nonsense mutations in cartilage tissue has been examined in two patients, demonstrating that the mutant mRNA is completely removed by nonsense mediated mRNA decay PMID: 15880705
    21. retinoids stimulate collagen X transcription IN chondrocytes PMID: 16598786
    22. the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation PMID: 16806867
    23. Type X collagen was not detected in any of atehrosclerotic plaques investigated in crural arteries. PMID: 17335825
    24. Investigated a family affected with the Spahr type of metaphyseal chondrodysplasia. Sequencing of RMRP, and a haplotype analysis using highly informative markers around the COL10A1 excluded both genes from being pathogenic in this family. PMID: 18553549
    25. HY(hypertrophy) box is the core element responsive to RUNX-2 in human COL10A1 promoter PMID: 19116917

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  • 相關(guān)疾?。?/div>
    Schmid type metaphyseal chondrodysplasia (SMCD)
  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2185

    OMIM: 120110

    KEGG: hsa:1300

    STRING: 9606.ENSP00000243222

    UniGene: Hs.520339