Human Complement Fragment 4d,C4d ELISA Kit

文獻(xiàn)引用 (1) 說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

人補體片段4d(C4d)酶聯(lián)免疫試劑盒（CSB-E14192h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4d含量。C4d是經(jīng)典補體激活途徑的相關(guān)產(chǎn)物，可反映補體系統(tǒng)激活狀況。研究機制上，其產(chǎn)生與C4的裂解有關(guān)，檢測C4d水平能輔助判斷補體激活程度，在免疫相關(guān)疾病，如移植排斥反應(yīng)等的診斷和病情監(jiān)測中有重要價值。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于體外研究場景，如探討補體系統(tǒng)在心血管疾病、腎臟病理或免疫復(fù)合物相關(guān)疾病中的作用機制，也可用于評估實驗動物模型或細(xì)胞實驗中補體激活水平，為免疫相關(guān)基礎(chǔ)研究提供精準(zhǔn)定量工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C4d
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4d in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	103
	Range %	94-108
1:200	Average %	98
	Range %	90-104
1:400	Average %	89
	Range %	82-93
1:800	Average %	95
	Range %	88-98

回收率：

The recovery of human C4d spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	113	103-117
EDTA plasma (n=4)	94	89-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.