Human Complement fragment 3c,C3c ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人補體片段3c(C3c)酶聯(lián)免疫試劑盒（CSB-E11168h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液、唾液樣本中的C3c含量。C3c是補體系統(tǒng)中的一個重要成分，參與經(jīng)典和替代途徑的補體激活。其在腫瘤免疫和病毒感染中發(fā)揮重要作用，如通過調節(jié)免疫反應和直接殺傷腫瘤細胞來抑制腫瘤生長，同時在HBV宮內傳播中與免疫復合物相互作用。C3c的研究有助于深入理解補體系統(tǒng)在免疫調節(jié)和疾病發(fā)生中的作用。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研領域中對補體系統(tǒng)功能的探索，例如在炎癥機制研究、疾病模型構建或藥物干預實驗中動態(tài)監(jiān)測補體激活狀態(tài)，也可用于體外培養(yǎng)體系中補體相關生物標志物的分析，為免疫調節(jié)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C3c
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, urine, saliva
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C3c in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	88
	Range %	84-92
1:10000	Average %	94
	Range %	90-98
1:20000	Average %	99
	Range %	95-104
1:40000	Average %	102
	Range %	96-108

回收率：

The recovery of human C3c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.