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  4. Human Corticosteroid Binding Globulin(CBG) ELISA Kit

Human Corticosteroid Binding Globulin(CBG) ELISA Kit

  • 中文名稱：
    人皮質(zhì)類固醇結(jié)合球蛋白( CBG )酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14214h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人皮質(zhì)類固醇結(jié)合球蛋白( CBG )酶聯(lián)免疫試劑盒（CSB-E14214h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPINA6含量。試劑盒檢測范圍為20 pmol/mL-8000 pmol/mL，適用于探索CBG在內(nèi)分泌調(diào)控、應(yīng)激反應(yīng)機(jī)制、肝臟功能及相關(guān)疾病模型中的表達(dá)變化，或用于評估藥物干預(yù)對激素運(yùn)輸?shù)鞍椎挠绊懷芯俊１酒穬H用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CBG ELISA Kit; CBG_HUMAN ELISA Kit; corticosteroid binding globulin ELISA Kit; Corticosteroid-binding globulin ELISA Kit; serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 ELISA Kit; Serpin A6 ELISA Kit; serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 ELISA Kit; Serpina6 ELISA Kit; Transcortin ELISA Kit
  • 縮寫：
    SERPINA6
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    20 pmol/mL-8000 pmol/mL
  • 靈敏度：
    20 pmol/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %91-100
    1:2Average %93
    Range %88-99
    1:4Average %93
    Range %89-100
    1:8Average %97
    Range %91-108
  • 回收率：
    The recovery of human CBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-99
    EDTA plasma (n=4)9289-95
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2Average
    80000.411 0.407 0.409
    15000.772 0.765 0.769
    2501.209 1.198 1.204
    701.588 1.584 1.586
    201.746 1.736 1.741
    02.462 2.466 2.464
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

相關(guān)問答

■ 常見問題解答
Q:

我對測量人類血漿樣本中的皮質(zhì)類固醇結(jié)合球蛋白很感興趣。我看了一下你們的產(chǎn)品CSB-E14214h，我有一些問題: １、試劑盒是針對完整的cbg有特異性嗎？還是可能與彈性蛋白酶裂解形式有交叉反應(yīng)？是單抗還是雙抗設(shè)計(jì)？抗體在哪里與蛋白質(zhì)結(jié)合？ 2、試劑盒檢測到的典型血漿水平是多少？ 3、我們需要測量約160個(gè)樣品，需要多少個(gè)這樣的ELISA試劑盒?

A:
產(chǎn)品CSB-E14214h不是雙抗試劑盒?？贵w信息如下: 一種為預(yù)包被的山羊抗兔二抗，另一種為特異性抗體-兔多克隆抗體。這是一種競爭性分析方法，而不是傳統(tǒng)的雙抗夾心法。具體結(jié)合位點(diǎn)尚不清楚，可檢測到CBG蛋白。 如果要測試160個(gè)樣品，并且樣品和標(biāo)準(zhǔn)品都做單孔，那么兩個(gè)96T就可以了。然而，考慮到樣品和可能的預(yù)實(shí)驗(yàn)要求和準(zhǔn)確性因素，建議對樣品進(jìn)行重復(fù)。這將需要更多的工具包。欲了解更多詳情，請與我們聯(lián)系，我們一起討論。

靶點(diǎn)詳情

  • 功能：
    Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that some individuals with adrenal insufficiency due to SERPINA6 mutations present with hypotension or nonspecific symptoms such as fatigue, nausea, or chronic pain, but symptomatology varies widely, even between family members with identical mutations. [REVIEW] PMID: 29194043
    2. In conclusion, site-specific CBG N-glycosylation regulates the bioavailability of cortisol in inflamed environments by fine-tuning the RCL proteolysis by endogenous and exogenous elastases. PMID: 27339896
    3. The results suggest reduced CBG cleavage in central obesity, possibly contributing to the characteristic inflammatory phenotype of the central obesity and metabolic syndrome. PMID: 27300474
    4. Corticosteroid-binding globulin cleavage may be pathogen-dependent in bloodstream infection PMID: 27887960
    5. Data indicate there was no significant circadian variation in serum cortisol-binding globulin (CBG) concentration in any of the 4 groups. PMID: 26603673
    6. Human cardiomyocytes express mineralocorticoid receptors, but are mostly free of nuclear glucocorticoid receptors. CBG is expressed in myocardium and in Purkinje fibers. CBG in heart is colocalized with mineralocorticoid receptor. PMID: 25251318
    7. SERPINA6-rs1998056 regulated by FOXA/ERalpha might be associated with female HCC risk PMID: 25198130
    8. genetic variation in the SERPINA6 gene may be associated with altered CBG levels during adolescence. PMID: 24691024
    9. the results suggest that DEX repression of Corticosteroid-binding globulin involves tethering of the glucocorticoid receptor to C/EBPb. PMID: 25335188
    10. 8 naturally occurring CBG mutants with abnormalities in cortisol-binding affinity, cortisol-binding capacity, production/secretion, sensitivity to proteolytic cleavage of the reactive center loop, or recognition by monoclonal antibodies used for ELISAs were identified PMID: 25322275
    11. The study investigate the half-life of intact and elastase cleaved CBG in the rabbit. The half-lives of intact and cleaved human CBG are 10 h and identical. PMID: 25636722
    12. CBG is coexpressed with hypothalamic neuropeptides.CBG may be involved in control of systemic and central stress response. PMID: 24246737
    13. Exome chip and 1000 Genomes imputation analysis of this locus in the CROATIA-Korcula cohort identified missense mutations in SERPINA6 and SERPINA1 that did not account for the effects of common variants PMID: 25010111
    14. Atomic interactions observed between progesterone and CBG explain the lower affinity of progesterone in comparison to corticosteroids. PMID: 23300763
    15. investigation of physicochemical, electronic, and structural requirements for selective and effective binding of steroids (many are endogenous steroid metabolites) to CBG and SHBG active/ligand-binding sites PMID: 22242800
    16. The influence of glucocorticoid receptor and corticosteroid-binding globulin gene polymorphisms in obesity and/or cortisol action in prepubertal obese children. PMID: 22576823
    17. three polymorphisms, -26 C/G, -54 C/T, and -144 G/C located close to the putative hepatic nuclear factor (HNF) 1 binding elements, altered the transactivation effect of HNF1beta PMID: 22932886
    18. genetic association studies in Chinese populations: Data suggest that SERPINA6 with SNP (A51V) is poorly produced/secreted; plasma corticosteroid-binding globulin levels in heterozygous subjects for this variant are 50% lower than in normal subjects. PMID: 22337907
    19. N-glycans are involved in the CBG:receptor interaction and indicates that the modulation is performed by steric and/or electrostatic means through the terminal neuraminic acid residues. PMID: 21558494
    20. We found that polymorphisms in SLC6A4, TPH2, and SERPINA6 appear to be maternal factors increasing the risk of having a child with facial clefts. PMID: 22072571
    21. Data show thatin chronic hepatitis C patients, IL28B polymorphism was associated with serum levels of corticosteroid binding globulin, a major transporter of cortisol. PMID: 21750736
    22. CBG influences the endocrine and neurobehavioral response to stress, including the development of pain/fatigue syndromes PMID: 21795453
    23. Allosteric modulation of hormone release from thyroxine and corticosteroid-binding globulins. PMID: 21325280
    24. Plasma cortisol responses attenuated more rapidly in the able-bodied men, compared to spinal cord-injured subjects, due to significant rise in circulating corticosteroid-binding globulin. PMID: 20839151
    25. SHBG, CBG and total cortisol and free plasma cortisol were measured and to investigate whether or not these analytes correlated with the degree of insulin resistance and the presence of the metabolic syndrome. PMID: 20354921
    26. Effect of mutations of SERPINA6 on cortisol-binding, and thermal and protease sensitivity are reported. PMID: 20226861
    27. findings support the hypothesis that the corticosteroid binding globulin level is an interesting indicator for both insulin resistance and low grade inflammation PMID: 12364459
    28. CBG-negative preadipocytes proliferated more rapidly and showed greater peroxisome proliferator-activated receptor-gamma-mediated differentiation than normal preadipocytes. CBG was not expressed in normal human preadipocytes. PMID: 12554596
    29. human Fallopian tube cells actively express and secrete a CBG-like progesterone-binding protein, which might play a role in the in vivo modulation of human sperm acrosome reaction PMID: 14871264
    30. Homozygosity for the serine allele of the CBG gene may predispose to chronic fatigue syndrome, perhaps due to an effect on hypothalamic-pituitary-adrenal axis function related to altered CBG-cortisol transport function or immune-cortisol interactions. PMID: 15554358
    31. Circulating adiponectin, CBG concentration, and fasting cortisol were significantly interrelated in healthy subjects. A significant sexual dimorphism exists in this association. PMID: 15877287
    32. We concluded that CBG gene polymorphisms might modulate the influence of the HPA axis on the fat mass distribution in obese women. PMID: 16222046
    33. To the best of our knowledge, this case represents the first de novo mutation reported for corticosteroid-binding globulin deficiency, implicating a pathogenic role of variants of SERPINA6 in some cases of muscle fatigue. PMID: 17245537
    34. A mechanism for the effect of CBG mutations on fatigue is not readily apparent because free cortisol levels are normal. PMID: 17547679
    35. The crystal structures of cleaved human CBG (cCBG) at 1.8-A resolution and its complex with cortisol at 2.3-A resolution, is reported. PMID: 18513745
    36. several substitutions (E334A, V336R, G340S, and T342P) increased the steroid binding affinities of human CBG even before elastase cleavage PMID: 19011238
    37. Results describe the expression of corticosteroid-binding globulin (CBG) in the low-grade malignant human astrocytoma cell line 1321N1. PMID: 19172388

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  • 相關(guān)疾?。?/div>
    Corticosteroid-binding globulin deficiency (CBG deficiency)
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 組織特異性：
    Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1540

    OMIM: 122500

    KEGG: hsa:866

    STRING: 9606.ENSP00000342850

    UniGene: Hs.532635