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  4. Human Cytokine-like protein 1(CYTL1) ELISA kit

Human Cytokine-like protein 1(CYTL1) ELISA kit

  • 中文名稱：
    人細(xì)胞因子樣蛋白1(CYTL1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006472HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人細(xì)胞因子樣蛋白1(CYTL1)酶聯(lián)免疫試劑盒（CSB-EL006472HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYTL1含量。CYTL1是一種小分子量的分泌蛋白，最初在CD34+細(xì)胞中發(fā)現(xiàn)。研究發(fā)現(xiàn)，CYTL1在細(xì)胞內(nèi)具有新型抑癌因子特征，通過穩(wěn)定NDUFV1抑制乳腺癌代謝重編程，其作用機(jī)制涉及與MDM2競爭性結(jié)合在NDUFV1的1-80結(jié)構(gòu)域，從而干擾NDUFV1的泛素化降解，實(shí)現(xiàn)穩(wěn)態(tài)的OXPHOS代謝。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可廣泛應(yīng)用于細(xì)胞因子調(diào)控機(jī)制研究、疾病動物模型中的蛋白表達(dá)動態(tài)分析，或體外培養(yǎng)細(xì)胞上清液中CYTL1分泌水平的定量評估，為探索其生物學(xué)功能及信號通路提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C17 ELISA Kit; C4orf4 ELISA Kit; CYTL1 ELISA Kit; CYTL1_HUMAN ELISA Kit; Cytokine like 1 ELISA Kit; Cytokine like protein C17 ELISA Kit; Cytokine-like protein 1 ELISA Kit; Protein C17 ELISA Kit
  • 縮寫：
    CYTL1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CYTL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 82-91    
    1:2 Average % 94    
    Range % 89-98    
    1:4 Average % 94    
    Range % 90-97    
    1:8 Average % 92    
    Range % 88-97    
  • 回收率：
    The recovery of human CYTL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 91-99    
    EDTA plasma (n=4) 103 98-108    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 2.008 2.102 2.055 1.877    
    5 1.735 1.797 1.766 1.588    
    2.5 1.408 1.369 1.389 1.211    
    1.25 1.121 1.068 1.095 0.917    
    0.625 0.737 0.728 0.733 0.555    
    0.312 0.488 0.468 0.478 0.300    
    0.156 0.336 0.331 0.334 0.156    
    0 0.178 0.178 0.178 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Circular dichroism analysis showed that, like chemokines, CYTL1has a higher content of beta-sheet than alpha-helix secondary structure; recombinant CYTL1 promoted calcium flux in chondrocytes; unlike chemokines, CYTL1 had limited affinity to proteoglycans; together, these properties further support cytokine-like properties for CYTL1 with some overlap with the chemokines PMID: 28478073
    2. this study shows that CYTL1 possesses chemotactic activity and demonstrates that its functional receptor is CCR2B PMID: 27084102
    3. when the native signal peptide of CYTL1 was replaced by IgGkappaSP, the expression and secretion was increased by 4 fold. PMID: 26922322
    4. role in regulation embryo implantation PMID: 26800213
    5. Cytl1 is highly expressed in the endometrium during the embryo implantation and its expression is regulated by ovarian hormones. Cytl1 enhances endometrial proliferation, induces the secretion of endometrial LIF and HB-EGF, and even enhances endometrial cell adhesion to trophoblastic cells. These indicate that Cytl1 is a potential molecular mediator of embryo is a potential molecular mediator in embryo implantation. PMID: 26800213
    6. our results showed the first evidence of CYTL1 expression in SH-SY5Y neuroblastoma cells and human NB tissues, revealed a possible link between CYTL1 and NB development PMID: 22797702
    7. evidence suggests that ANKRD7 and CYTL1 genes may play an important role in the variance in alcohol drinking risk PMID: 22613542
    8. C17orf62-L could induce cell death accompanied with rising of cleaved PARP. PMID: 21503106
    9. The DNA methylation pattern of the CYTL1 promoter region was significantly different between early and advanced stages of squamous cell carcinoma. PMID: 22011669
    10. C17 as a cytokine that s contributes to immune homeostasis systemically or in a tissue-specific manner in the joint PMID: 21799806
    11. We report a structural-functional characterization of cytokine-like protein 1 (Cytl1) by a combination of different computational structure-based techniques. PMID: 21322034

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  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Specifically expressed in CD34+ hematopoietic cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 24435

    OMIM: 607930

    KEGG: hsa:54360

    STRING: 9606.ENSP00000303550

    UniGene: Hs.13872