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  4. Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

  • 中文名稱：
    人D-乳酸脫氫酶(D-LDH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11720h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人D-乳酸脫氫酶(D-LDH)酶聯(lián)免疫試劑盒（CSB-E11720h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LDHD含量。人D-乳酸脫氫酶（D-LDH）在腫瘤代謝中起關(guān)鍵作用，其研究機(jī)制涉及在厭氧條件下催化D-乳酸生成丙酮酸，影響腫瘤生長(zhǎng)和代謝。D-LDH基因敲除可提高D-乳酸產(chǎn)量，純度高達(dá)99%以上，為D-乳酸工業(yè)化生產(chǎn)提供新途徑。試劑盒檢測(cè)范圍為31.25 mIU/mL-2000 mIU/mL，該產(chǎn)品可廣泛應(yīng)用于腸道功能研究、代謝通路探索、疾病模型構(gòu)建等方向，例如評(píng)估缺血再灌注損傷、腸道屏障功能障礙或微生物代謝研究中D - LDH的動(dòng)態(tài)變化，為能量代謝調(diào)控機(jī)制及病理生理研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit
  • 縮寫：
    LDHD
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 mIU/mL-2000 mIU/mL
  • 靈敏度：
    7.8 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %87-103
    1:2Average %91
    Range %86-103
    1:4Average %94
    Range %86-105
    1:8Average %91
    Range %84-100
  • 回收率：
    The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9791-106
    EDTA plasma (n=4)9686-106
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    20002.418 2.385 2.402 2.265
    10001.773 1.728 1.751 1.614
    5000.936 1.002 0.969 0.832
    2500.624 0.651 0.638 0.501
    1250.407 0.422 0.415 0.278
    62.50.282 0.287 0.285 0.148
    31.250.253 0.264 0.259 0.122
    00.135 0.139 0.137
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in D-lactate, but not L-lactate catabolic process.
  • 基因功能參考文獻(xiàn)：
    1. The activity and expression of the mitochondrial D-lactate dehydrogenase, is higher in prostate cancer versus normal prostate cells. PMID: 23333299
    2. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
    3. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
  • 亞細(xì)胞定位：
    Mitochondrion.
  • 蛋白家族：
    FAD-binding oxidoreductase/transferase type 4 family
  • 組織特異性：
    Expressed moderately in heart and liver and at lower levels in skeletal muscle and kidney.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 19708

    OMIM: 607490

    KEGG: hsa:197257

    STRING: 9606.ENSP00000300051

    UniGene: Hs.380929