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Human Defensin-6(DEFA6) ELISA kit

  • 中文名稱：
    人防御素6(DEFA6)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006658HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人防御素6(DEFA6)酶聯(lián)免疫試劑盒（CSB-EL006658HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DEFA6含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于探究腸道疾病模型、病原體感染或免疫調(diào)控實驗中DEFA6的表達動態(tài)，為研究其抗菌機制、黏膜免疫應答及宿主 - 微生物互作提供可靠工具，特別適用于體外實驗的蛋白質(zhì)水平檢測及生物標志物篩選等科研用途。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    alpha 6 ELISA Kit; DEF6 ELISA Kit; DEF6_HUMAN ELISA Kit; DEFA 6 ELISA Kit; DEFA6 ELISA Kit; Defensin 6 ELISA Kit; Defensin alpha 6 ELISA Kit; Defensin alpha 6 Paneth cell specific ELISA Kit; Defensin ELISA Kit; Defensin-6 ELISA Kit; HD 6 ELISA Kit; HD6 ELISA Kit
  • 縮寫：
    DEFA6
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 91-99    
    1:2 Average % 103    
    Range % 98-106    
    1:4 Average % 110    
    Range % 106-114    
    1:8 Average % 92    
    Range % 89-97    
  • 回收率：
    The recovery of human DEFA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 84 81-89    
    EDTA plasma (n=4) 97 93-100    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 2.217 2.104 2.161 2.047    
    5 1.762 1.752 1.757 1.643    
    2.5 1.253 1.198 1.226 1.112    
    1.25 0.849 0.856 0.853 0.739    
    0.625 0.528 0.534 0.531 0.417    
    0.312 0.354 0.368 0.361 0.247    
    0.156 0.244 0.238 0.241 0.127    
    0 0.115 0.112 0.114 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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靶點詳情

  • 功能：
    Has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. May protect cells against infection with HIV-1.
  • 基因功能參考文獻：
    1. HD6 assembled into nanonets can prevent key steps in C. albicans pathogenesis PMID: 28198610
    2. alpha-Defensin 6 Self-Assembly Prevents Adhesion and Suppresses Virulence Traits of Candida albicans PMID: 28026958
    3. Downregulation of HD6 in noninflamed mucosa may contribute to mucosal barrier dysfunction of patients with Crohn's Disease. PMID: 26891258
    4. Paneth cell alpha-defensin 6 is an antimicrobial peptide. PMID: 25354318
    5. Native HD-6 readily self-assembles into elongated fibrils observable by transmission electron microscopy, agglutinates both Gram-negative and -positive bacteria, and prevents the Listeria monocytogenes from invading cultured cells. PMID: 25158166
    6. In the meta-analysis, DEFA6 rs13275170 and DEFB1 rs2738169 had both a 1.3-fold increased odds ratio for gastric cancer. PMID: 23028900
    7. study reports that HD6 affords protection against invasion by enteric bacterial pathogens in vitro and in vivo; after stochastic binding to bacterial surface proteins, HD6 undergoes ordered self-assembly to form fibrils and nanonets that surround and entangle bacteria PMID: 22722251
    8. Regions 2, 3 & 9, 10 were more hydrophobic than other regions. It was difficult for the HD molecule to have many conformations because of its 3 S-S bridges; however, it responds to various external factors by restricting conformation. PMID: 21873175
    9. Defensin alpha 6 (DEFA 6) may have a role in adenoma but not in progression to colon carcinoma PMID: 20979654
    10. defensin alpha6 is highly expressed in colon cancer cell lines PMID: 15613481
    11. Crystal structure of HD6. PMID: 17088326
    12. Human enteric defensin (HD)-5 and HD-6 were detected in Paneth cells, which are observed in the gastric metaplastic mucosa as well as small intestinal epithelia. PMID: 19250512

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Alpha-defensin family
  • 組織特異性：
    Paneth cells of the small intestine.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2765

    OMIM: 600471

    KEGG: hsa:1671

    STRING: 9606.ENSP00000297436

    UniGene: Hs.711