Human Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

人脫氫表雄酮硫酸酯(DHEA-S)酶聯(lián)免疫試劑盒（CSB-E05105h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DHEA-S含量。DHEA - S即硫酸脫氫表雄酮，是腎上腺分泌的一種類固醇激素。它在人體代謝、免疫等生理過程中發(fā)揮作用。研究機制上，其參與多種信號通路，與激素平衡、細胞增殖等密切相關(guān)，在衰老、代謝疾病等領(lǐng)域是研究熱點。試劑盒檢測范圍為20 ng/mL-4000 ng/mL，適用于基礎(chǔ)研究領(lǐng)域中對激素代謝機制、內(nèi)分泌系統(tǒng)功能評估、藥物干預(yù)效果分析等科研項目，為探索腎上腺功能、衰老相關(guān)激素變化或代謝性疾病模型提供可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

DHEA-S
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

20 ng/mL-4000 ng/mL
靈敏度：

10 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-103
1:2	Average %	96
	Range %	89-105
1:4	Average %	94
	Range %	90-100
1:8	Average %	90
	Range %	83-110

回收率：

The recovery of human DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-103
EDTA plasma (n=4)	89	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.