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  4. Human Deoxyribonuclease gamma(DNASE1L3) ELISA kit

Human Deoxyribonuclease gamma(DNASE1L3) ELISA kit

  • 中文名稱：
    人脫氧核糖核酸酶γ(DNASE1L3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL007052HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人脫氧核糖核酸酶γ(DNASE1L3)酶聯(lián)免疫試劑盒（CSB-EL007052HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的DNASE1L3含量。DNASE1L3 是一種重要靶點(diǎn)。它屬于脫氧核糖核酸酶家族，在維持核酸代謝平衡等方面有一定作用。研究機(jī)制方面，其主要通過降解特定核酸來發(fā)揮功能，異常表達(dá)或活性改變可能與自身免疫性疾病等病理過程存在關(guān)聯(lián)。試劑盒檢測范圍為23.44 pg/mL-1500 pg/mL，適用于基礎(chǔ)醫(yī)學(xué)研究中探索DNASE1L3在疾病模型中的表達(dá)調(diào)控、評估藥物干預(yù)對核酸酶活性的影響，或用于體外培養(yǎng)細(xì)胞凋亡過程中相關(guān)分子機(jī)制的研究，為免疫學(xué)、腫瘤學(xué)及細(xì)胞生物學(xué)領(lǐng)域提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Deoxyribonuclease gamma ELISA Kit; Deoxyribonuclease I like 3 ELISA Kit; Deoxyribonuclease I like III ELISA Kit; Deoxyribonuclease I-like 3 ELISA Kit; DHP 2 ELISA Kit; DHP2 ELISA Kit; DNAS1L3 ELISA Kit; DNase gamma ELISA Kit; DNase I homolog protein 2 ELISA Kit; DNase I homolog protein DHP2 ELISA Kit; DNase I like 3 ELISA Kit; DNase I-like 3 ELISA Kit; DNASE1L3 ELISA Kit; DNSL3_HUMAN ELISA Kit; Liver and spleen DNase ELISA Kit; LS DNase ELISA Kit; LS-DNase ELISA Kit; LSD ELISA Kit; SLEB 16 ELISA Kit; SLEB16 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    23.44 pg/mL-1500 pg/mL
  • 靈敏度：
    5.86 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DNASE1L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-95
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 97
    Range % 91-103
  • 回收率：
    The recovery of human DNASE1L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-98
    EDTA plasma (n=4) 97 90-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.634 2.538 2.586 2.500
    750 2.223 2.302 2.263 2.177
    375 1.817 1.835 1.826 1.740
    187.5 1.117 1.204 1.161 1.075
    93.75 0.759 0.748 0.754 0.668
    46.88 0.484 0.498 0.491 0.405
    23.44 0.277 0.261 0.269 0.183
    0 0.087 0.085 0.086 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能參考文獻(xiàn)：
    1. The authors found a significant reduction in serum DNase1l3 level in dermatomyositis/polymyositis and systemic lupus erythematosus, which may associate with clinic features and disease activity. PMID: 28039554
    2. Data indicate a likely functional variant influencing scleroderma susceptibility at deoxyribonuclease I-like 3 protein DNASE1L3 missense polymorphism rs35677470. PMID: 25332064
    3. Single nucleotide polymorphisms in the DNASE1L3 is associated with autoimmune diseases. PMID: 24206041
    4. DNASE1L3 mutations occur in hypocomplementemic urticarial vasculitis syndrome. PMID: 23666765
    5. We identified a rare autosomal recessive form of systemic lupus erythematosus, in which autozygome analysis revealed a null mutation in the DNASE1L3 gene PMID: 22019780
    6. investigates the distribution of DNASE1L3 gene SNPs in exons of the gene in eight Asian, three African, and three Caucasian populations worldwide using newly devised genotyping methods. PMID: 21692081
    7. The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells. PMID: 12154052
    8. Identification of two functional nuclear localization signals in DNase gamma that have a role in apoptotic DNA fragmentation. PMID: 12943533
    9. DNase gamma is involved in the generation of resected double-strand DNA breaks associated with somatic hypermutation PMID: 15629432
    10. DNAS1L3 plays an active role in lymphoma cell sensitization to VP-16 and its deficiency may constitute a novel mechanism of drug resistance in these cells PMID: 16427601
    11. A Caucasian-specific allele in SNP R206C produces an inactive form of DNase Il3. PMID: 19559017
    12. Results distinguish DNase gamma-dependent and CAD/DFF40-dependent DNA fragmentations, and show that even if DNA fragmentation is initiated by CAD/DFF40, DNase gamma is required for the more complete digestion of the genomic DNA in dying cells. PMID: 19574717

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  • 相關(guān)疾病：
    Systemic lupus erythematosus 16 (SLEB16)
  • 亞細(xì)胞定位：
    Nucleus. Endoplasmic reticulum. Secreted.
  • 蛋白家族：
    DNase I family
  • 組織特異性：
    Liver and spleen.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2959

    OMIM: 602244

    KEGG: hsa:1776

    STRING: 9606.ENSP00000316193

    UniGene: Hs.476453