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  4. Human Desmoglein-3(DSG3) ELISA kit

Human Desmoglein-3(DSG3) ELISA kit

  • 中文名稱：
    人橋粒芯蛋白3(DSG3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL007205HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人橋粒芯蛋白3(DSG3)酶聯(lián)免疫試劑盒（CSB-EL007205HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DSG3含量。DSG3（橋粒芯糖蛋白 3）是一種重要的細(xì)胞黏附分子，在維持上皮組織完整性方面發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)其異常表達(dá)與多種疾病尤其是天皰瘡密切相關(guān)，機制上可能涉及自身抗體與 DSG3 結(jié)合，破壞細(xì)胞間黏附，為相關(guān)疾病研究和治療提供了靶點思路。試劑盒檢測范圍為28 pg/mL-1800 pg/mL，適用于科研領(lǐng)域研究DSG3在皮膚屏障功能、上皮組織損傷修復(fù)或自身免疫反應(yīng)中的動態(tài)變化，例如通過檢測疾病模型或?qū)嶒灨深A(yù)后樣本中的DSG3水平，揭示其在細(xì)胞黏附調(diào)控及病理過程中的作用，為分子機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    130 kD pemphigus vulgaris antigen ELISA Kit; 130 kDa pemphigus vulgaris antigen ELISA Kit; Balding ELISA Kit; Cadherin family member 6 ELISA Kit; CDHF 6 ELISA Kit; CDHF6 ELISA Kit; Desmoglein 3 ELISA Kit; Desmoglein 3 precursor ELISA Kit; Desmoglein-3 ELISA Kit; Desmoglein3 ELISA Kit; DKFZp686P23184 ELISA Kit; DSG 3 ELISA Kit; DSG3 ELISA Kit; DSG3_HUMAN ELISA Kit; Pemphigus vulgaris antigen ELISA Kit; PVA ELISA Kit
  • 縮寫：
    DSG3
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    28 pg/mL-1800 pg/mL
  • 靈敏度：
    7 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DSG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-95
    1:2Average %102
    Range %97-105
    1:4Average %91
    Range %85-94
    1:8Average %97
    Range %92-101
  • 回收率：
    The recovery of human DSG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-97
    EDTA plasma (n=4)9590-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    18002.826 2.778 2.802 2.696
    9002.090 2.000 2.045 1.939
    4501.157 1.164 1.161 1.055
    2250.518 0.498 0.508 0.402
    1120.335 0.323 0.329 0.223
    560.219 0.214 0.217 0.111
    280.147 0.142 0.145 0.039
    00.105 0.107 0.106
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • 基因功能參考文獻(xiàn)：
    1. flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
    2. our findings suggest that Dsg3 functions as a key in cell-cell adhesion through at least a mechanism of regulating E-cadherin membrane trafficking PMID: 27254775
    3. Desmoglein 3 Order and Dynamics in Desmosomes PMID: 29212005
    4. Data indicate that all 3 monoclonal antibodies (mAbs) targeted the same extracellular cadherin (EC) domain on desmoglein 3 (Dsg3), caused Dsg3 internalization in primary keratinocytes, and caused suprabasal blisters in skin. PMID: 27304671
    5. DSG3 was a key prognostic factor and predictor for neoadjuvant concurrent chemoradiotherapy response in rectal cancer patients. PMID: 27040321
    6. Treg cells exert a clear down-regulatory effect on the Dsg3-driven T-cell response and, accordingly, the formation of Dsg3-specific IgG antibodies PMID: 26661498
    7. Data identify DSG3 as a negative prognostic biomarker in resected pancreatic ductal adenocarcinoma, as high DSG3 expression is associated with poor overall survival and poor tumour-specific survival. PMID: 26469831
    8. this study directly demonstrates the occurrence of desmoglein 3 molecules outside of desmosomes and reveales that the adhesive properties of these molecules do differ depending on their localization. PMID: 25510735
    9. DSG2 and DSG3 might be potential diagnostic markers for squamous cell carcinoma of the lung and DSG3 could be a potential differentiation marker for lung cancer. PMID: 25468811
    10. alterations of their expression suggest a role of Dsg3 and gamma-catenin (additionally to E-cadherin/beta-catenin) as biomarkers of malignant transformation risk of oral dysplasia and the biological behavior (aggressiveness) of oral cancer, respectively PMID: 23430339
    11. the Dsg3 epitopes targeted by pathogenic mPV IgG are human specific in mice PMID: 25695683
    12. Pemphigus vulgaris is characterized by low IgG reactivities to specific self-antigens along with high IgG reactivity to desmoglein 3. PMID: 24820664
    13. High DSG3 mRNA expression is associated with esophageal squamous cell carcinoma. PMID: 24568510
    14. Pemphigus vulgaris autoimmune globulin induces Src-dependent tyrosine-phosphorylation of PKP3 and its detachment from DSG3. PMID: 24328683
    15. Dsg3 did not associate with rafts in cells lacking desmosomal proteins. PMID: 24498201
    16. this study identifies a novel Dsg3-mediated c-Jun/AP-1 regulatory mechanism and PKC-dependent Ezrin phosphorylation that could be responsible for Dsg3-associated cancer metastasis. PMID: 23752190
    17. DSG3 functions as an oncogene and facilitates cancer growth and invasion in head and neck cancer cells PMID: 23737966
    18. The conformational Dsg3 ELISA index reflected the pathogenicity of anti-Dsg3 antibodies more accurately than the conventional Dsg3 ELISA index in pemphigus vulgaris. PMID: 23602628
    19. MAC387 and desmoglein-3 are reliable diagnostic markers for supporting the morphologic impression of squamous differentiation in urinary bladder carcinoma. PMID: 23806524
    20. All 3 markers were studied independently and were associated with tumor percentage in metastatic lymph nodes. PVA had the strongest correlation, followed by PTHrP and then TACSTD1. PMID: 23625795
    21. provide a proof of principle supporting that ultrasensitive nanostructured assay systems for DSG3 can be exploited to detect micrometastatic HNSCC lesions in lymph nodes, which can improve the diagnosis PMID: 23010602
    22. The cis-adhesive interface on extracellular subdomain EC1 recognized by the pathogenic antibody PVA224 is the primary target of the autoantibodies present in the serum of pemiphigus vulgaris patients. PMID: 22996451
    23. an important novel function for Dsg3 in promoting actin dynamics through regulating Rac1 and Cdc42 activation in epithelial cells. PMID: 22796473
    24. a useful immunohistochemical marker for differentiation of lung squamous cell carcinoma and adenocarcinoma from other subtypes PMID: 22495379
    25. In paraneoplastic pempigus and pemphigus vulgaris, IgG autoantibodies to DSG3 may be found in autoantibody profiling. PMID: 22318391
    26. Report mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides. PMID: 22261006
    27. These findings suggest a novel function for Dsg3 in the control of E-cadherin-Src signalling and cell-cell adhesion. PMID: 22294297
    28. These data indicate a contribution of Dsg depletion to pemphigus vulgaris pathogenesis dependent on Ca(2+)-induced differentiation. PMID: 21864491
    29. Silencing desmoglein 3 caused defects in cell-cell adhesion and concomitant reduction in cell proliferation in both HaCaT and MDCK cells. PMID: 21702856
    30. Dsg3, as an up-stream regulator of Src activity, helps regulate adherens junction formation through its interaction with E-cadherin PMID: 21151980
    31. The genetic background of the local population may explain why pemphigus occurs more commonly than bullous pemphigoid in Northwestern Romania compared with the population of Western Europe PMID: 20618495
    32. results argue against the hypothesis that DSG3 coding variants play a role in pemphigus vulgaris susceptibility PMID: 19678820
    33. Data strongly support the view that desmoglein 3 contributes to the regulation of epidermal differentiation. PMID: 12138195
    34. The appearance of DSG3-reactive Th2 cells is constant at different stages of pemphigus vulgaris (PV), while DSG3-reactive Th1 cells are detected at a significantly higher frequency in chronic active PV. PMID: 12496453
    35. In trichilemmal keratinization in follicle, and in cysts in these areas, desmoglein 3 expressed throughout outer root sheath and cyst wall. In areas of epidermal-like keratinization, desmoglein 3 expression was limited mainly to the basal layer. PMID: 12787134
    36. a novel negative marker for epidermal stem cell-containing population of keratinocytes PMID: 12953062
    37. Dsg3(AA145-192)-specific cells preferentially utilize the TCRVbeta13 gene, while Dsg3(AA240-303)- and Dsg3 (AA570-614)-specific cells utilize Vbeta7 and Vbeta17 genes, respectively PMID: 14675184
    38. The five genes, and three of the encoded proteins, were shown differentially expressed between a group of keratoconus patients and a reference group using different techniques PMID: 16015083
    39. Dsg3 endocytosis, keratin filament retraction, and the loss of keratinocyte cell-cell adhesion are coordinated responses to PV IgG PMID: 16377623
    40. autoantibodies against desmoglein 3 (Dsg3) have been detected in sera from patients with fogo selvagem PMID: 16763546
    41. analysis of desmoglein 3 ectodomain in pemphigus vulgaris PMID: 16842599
    42. Overexpression of DSG3 is associated with head and neck cancer PMID: 16878157
    43. Desmoglein 3 status indicated a poor prognosis in lung cancers. PMID: 17084439
    44. The T cellular autoimmune response against immunodominant peptides of Dsg3 in patients with pemphigus vulgaris is monitored with highly specific HLA-DRbeta1*0402 tetramers. PMID: 17113829
    45. These data indicate that Dsg3(dim) populations from primary human adult keratinocytes and long-term established keratinocyte lines possess certain stem/progenitor cell-like properties. PMID: 17255524
    46. Data suggest that although the desmoglein (Dsg)3 depletion is not indicative for adhesive strength, it may indicate pathogenic changes within the cell and it plays a role in skin fragility or susceptibility to blister formation in pemphigus patients. PMID: 17428808
    47. Reduction of Dsg3 might be relevant to blister formation in pemphigus vulgaris. PMID: 17431647
    48. pemphigus vulgaris-IgG-dependent uPA activation is not related to anti-Dsg3 antibody activity PMID: 17532189
    49. Autoantibody from pemphigus vulgaris sera react with non-conformational epitopes of desmoglein 3. PMID: 18095943
    50. results indicate that the membrane proximal region in the IA region of Dsg3 is necessary for complex formation with P120-catenin and to maintain free Dsg3 at the cell surface before it is integrated into desmosomes PMID: 18343367

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
  • 組織特異性：
    Epidermis, tongue, tonsil, esophagus and carcinomas.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3050

    OMIM: 169615

    KEGG: hsa:1830

    STRING: 9606.ENSP00000257189

    UniGene: Hs.1925