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  4. Human Disintegrin and metalloproteinase domain-containing protein 2(ADAM2) ELISA kit

Human Disintegrin and metalloproteinase domain-containing protein 2(ADAM2) ELISA kit

  • 中文名稱：
    人解整合素樣金屬蛋白酶2(ADAM2)酶聯免疫試劑盒
  • 貨號：
    CSB-EL001280HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人解整合素樣金屬蛋白酶2(ADAM2)酶聯免疫試劑盒（CSB-EL001280HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADAM2含量。試劑盒檢測范圍為0.312 ng/ml- 20 ng/ml，該產品廣泛應用于生殖生物學研究、細胞遷移與分化機制探索、疾病模型構建等領域，尤其適用于探究ADAM2在精子發(fā)生、受精過程或腫瘤微環(huán)境中的功能調控。科研人員可通過高效完成樣本中ADAM2的動態(tài)監(jiān)測，為相關基礎研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    A disintegrin and metalloproteinase domain 2 ELISA Kit; ADAM 2 ELISA Kit; ADAM metallopeptidase domain 2 ELISA Kit; Adam2 ELISA Kit; ADAM2_HUMAN ELISA Kit; AI323749 ELISA Kit; Cancer/testis antigen 15 ELISA Kit; CRYN1 ELISA Kit; CRYN2 ELISA Kit; CT15 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 2 ELISA Kit; Fertilin beta ELISA Kit; Fertilin subunit beta ELISA Kit; Ftnb ELISA Kit; PH 30 ELISA Kit; PH 30b ELISA Kit; PH-30 ELISA Kit; PH30 ELISA Kit; Ph30 beta ELISA Kit; PH30-beta ELISA Kit
  • 縮寫：
    ADAM2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/ml - 20 ng/ml
  • 靈敏度：
    0.078 ng/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    101

    Range %

    97-105

    1:2

    Average %

    106

    Range %

    102-111

    1:4

    Average %

    90

    Range %

    86-96

    1:8

    Average %

    97

    Range %

    90-104

  • 回收率：

    The recovery of human ADAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    102

    95-109

    EDTA plasma (n=4)

    96

    91-103

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.305

    2.234

    2.269

    2.100

    10

    1.989

    1.967

    1.978

    1.809

    5

    1.447

    1.388

    1.418

    1.248

    2.5

    0.788

    0.779

    0.783

    0.614

    1.25

    0.433

    0.421

    0.427

    0.258

    0.625

    0.330

    0.323

    0.327

    0.157

    0.312

    0.266

    0.274

    0.270

    0.101

    0

    0.173

    0.165

    0.169

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.
  • 基因功能參考文獻：
    1. ADAM2, CALR3 and SAGE1 cancer/testis antigens are not promising targets for immunotherapy of breast and lung cancer. PMID: 26252478
    2. The fertilin beta contributes not only to successful fertilization, but may has an important impact in development of preimplantation embryos. PMID: 18292840
  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein.
  • 組織特異性：
    Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.
  • 數據庫鏈接：

    HGNC: 198

    OMIM: 601533

    KEGG: hsa:2515

    STRING: 9606.ENSP00000265708

    UniGene: Hs.177959