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中文名稱:人E選擇素(E-Selectin/CD62E)酶聯(lián)免疫試劑盒
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貨號:CSB-E04540h
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人E選擇素(E-Selectin/CD62E)酶聯(lián)免疫試劑盒(CSB-E04540h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SELE含量。SELE 即選擇素 E,主要表達于活化的血管內(nèi)皮細胞。其背景在于在炎癥反應(yīng)中起關(guān)鍵作用。研究機制方面,它能介導(dǎo)白細胞與內(nèi)皮細胞的初始黏附,促使白細胞滾動,利于白細胞遷移至炎癥部位,在炎癥、腫瘤轉(zhuǎn)移等病理過程研究中備受關(guān)注。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,靈敏度為0.078 ng/mL。適用于體外細胞模型、動物實驗或臨床前研究中內(nèi)皮細胞激活狀態(tài)的動態(tài)監(jiān)測,也可用于炎癥性疾病模型的生物標志物分析或藥物干預(yù)效果的評估,支持科研實驗室開展多樣本、多批次的E選擇素表達水平檢測需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:SELE; ELAM1; E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD antigen CD62E
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(ng/ml)
2.382
2.629
2.463
2.564
2.592
2.546
SD
0.041
0.043
0.036
0.049
0.052
0.047
CV(%)
5.76
5.61
4.93
6.52
6.86
6.28
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human E-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
99
Range %
95-110
1:2
Average %
95
Range %
91-103
1:4
Average %
87
Range %
82-92
1:8
Average %
94
Range %
89-98
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回收率:
The recovery of human E-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-99
Heparin plasma (n=4)
98
90-101
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標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
20
2.370
2.274
2.322
2.154
10
1.859
1.779
1.819
1.651
5
1.229
1.209
1.219
1.051
2.5
0.740
0.734
0.737
0.569
1.25
0.437
0.431
0.434
0.266
0.625
0.347
0.339
0.343
0.175
0.312
0.207
0.204
0.206
0.038
0
0.168
0.167
0.168
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Higher serum PGE2 is a predicative biomarker for postoperative delirium following elective orthopedic surgery in elderly patients M Mao,BMC geriatrics,2022
- KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke Zhanga X, et al,/,2019
- Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs Esplugas R, et al,Anticancer Research,2019
- Pomegranate Juice Increases Sirtuin1 Protein in Peripheral Blood Mononuclear Cell from Patients with Type 2 Diabetes: A Randomized Placebo Controlled Clinical Trial Sohrab Golbon.et al,METAB SYNDR RELAT D,2018
- Predictive Value of Combined LIPS and ANG-2 Level in Critically Ill Patients with ARDS Risk Factors Zhi Xu.et al,Mediators of Inflammation,2018
- Predictive value of combined LIPS and ANG-2 in critically ill patients with ARDS risk factors Zhi Xu.et al,/,/
- The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium Layne K.et al,Oncotarget,2017
- MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin Expression Deng H.et al,Cell Physiol Biochem.,2017
- Chemical constituents and sources of ambient particulate air pollution and biomarkers of endothelial function in a panel of healthy adults in Beijing, China. Wu S.et al,Sci Total Environ.,2016
相關(guān)產(chǎn)品
靶點詳情
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最新研究進展:SELE是選擇素E的縮寫,是一種黏附分子,可以調(diào)節(jié)白細胞的黏附和遷移,最近的研究表明其在多種疾病的發(fā)生和發(fā)展中具有重要作
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功能:Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
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基因功能參考文獻:
- Results highlight novel mechanisms by which p38 downregulates the expression of E-selectin through miR-146a and miR-31 following inflammatory stimuli associated to cancer progression. PMID: 29402939
- Levels of E and P selectin showed positive correlation with the length of stay. PMID: 29517629
- Our study shows a significant association of E-selectin gene polymorphism(Leu554Phe) with essential hypertension. PMID: 29178542
- The highest levels of sL- and sE-selectin were observed in colorectal patients with lymph node metastasis. PMID: 27775438
- Circulating CD62E(+) microparticle levels are abnormal in subjects with pre-diabetes. PMID: 28927403
- sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID: 29068581
- Neutrophil rolling over E-selectin at precise shear stress transmits tension and catch-bond formation with L-selectin via sLe(x), resulting in focal clusters that deliver a distinct signal to upshift beta2-integrins to a high-affinity state. Rivipansel effectively blocked formation of selectin catch-bonds, revealing a novel mechanotransduction circuit PMID: 28811304
- E-selectin-dependent rolling promotes adhesion to HA by both physically slowing cells and enabling them to position proximal to the surface, thereby increasing the on rate of adhesion. PMID: 28765175
- High E-selectin expression is associated with breast cancer metastasis. PMID: 27220365
- his study shows that two adhesion molecules, shed as soluble forms, are elevated during the acute phase of leptospirosis: E-selectin and s-ICAM1. These molecules may interfere with the process of immune cell recruitment to clear Leptospira at tissue levels. PMID: 28686648
- ELAM-1 upregulation may promote disorder in adhesion, and migration of the cells leading to the endometriosis disorder. PMID: 27433816
- Elevated endothelium-related mediators (vWF, E-selectin and EPCR) appear to participate in the development of pancreatic necrosis and may be a potential indicator of overall prognosis. PMID: 28007459
- interplay between flow-dependent on-rate and off-rate of E-selectin-ligand bonds determine flow-enhanced cell rolling stability PMID: 27558713
- High SELE expression is associated with Inflamed Atherosclerotic Plaque. PMID: 27237221
- In this study, we addressed this question by analysing sLex expression together with two glycoproteins (BST-2 and LGALS3BP), shown to interact with E-selectin in a carbohydrate-dependent manner, in a cohort of 249 invasive breast cancers PMID: 27176937
- SELE expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
- E-selectin concentrations in this study were associated with hyperglycemia, possibly reflecting early endothelial damage. E-selectin was not useful to assess carotid intima-media thickness, a marker of subclinical atherosclerosis, which appeared to be determined by aging and male gender. PMID: 27141013
- Plasma E-selectin is overexpressed in pediatric patients with mycoplasma pneumoniae pneumonia and myocardial injury. PMID: 26843511
- The significant presence of CLA+ T cells and E-selectin (CD62E) expressions in the OLPG suggests their involvement in the etiopathogenesis of oral lichen planus ; however, only a weak correlation between CLA+ T cells and E-selectin was observed. PMID: 26332489
- BD patients have a lower expression of ICAM-1, VCAM-1, and E-selectin in their tumor tissues. BD-HDL facilitates the adhesion of tumor cells to vascular endothelium by upregulating the expression of ICAM-1 and VCAM-1, thereby promoting the initial progression of breast cancer metastasis PMID: 26872904
- The E-selectin S149R polymorphisms is associated with the oncogenesis of breast cancer. PMID: 26828984
- Neisseria meningitidis caused a high level of E-selectin expression elicited by the activity of phosphorylated ATF2 transcription factor on the E-selectin promoter. PMID: 26153406
- E-selectin is more effective than cTnI in diagnosing myocardial injury in children. PMID: 26617843
- Inhibition of E-selectin expression allows an anti-tumoral effect on sLex-expressing hepatocellular carcinoma tumors in vivo PMID: 26988221
- Glycosphingolipids likely contribute to human myeloid cell adhesion to E-selectin under fluid shear, particularly the transition of rolling cells to firm arrest. PMID: 26868209
- Data show that both mucin16 (MUC16) and podocalyxin (PODXL)-E-selectin-mediated interactions are mechanically stronger than like L-selectin interactions at the single-molecule level. PMID: 26329844
- These findings provide consistent evidence that A516C and G98T polymorphisms of the SELE gene may be associated with increased susceptibility of coronary artery disease. PMID: 26937892
- Glaucomatous MYOC mutations activate the IL-1beta/NF-kappaB inflammatory stress response and the glaucoma marker SELE in trabecular meshwork cells. PMID: 26396484
- P. gingivalis was shown to activate NOD1, NOD2, and TLR2 expression, resulting in increased E-selectin expression in endothelial cells; direct inhibition of NF-kappaB and P38 MAPK significantly attenuated E-selectin expression induced by P. gingivalis in endothelial cells PMID: 25939768
- The number of E-selectin-positive cells was significantly higher in the tuberculoid form than in the lepromatous form of leprosy skin lesions. PMID: 26051909
- Gene-based study revealed significant associations of the SELE gene with Blood Pressure phenotypes for the first time among the Chinese Han population. PMID: 25424718
- This study showed that no significant differences in F206L Polymorphism of l-Selectin Between the Patients with Bronchial Asthma and Control. PMID: 24798295
- Blood ESEL was higher in pregnant women with preeclampsia, and non-pregnant women with a history of preeclampsia than women without preeclampsia or a history of preeclampsia. PMID: 26116870
- The findings suggest that C allele carriers of the SELE gene polymorphism (A561C) might be predisposed to EH in the Chinese population. PMID: 25171839
- E-selectin promoter was highly and specifically acetylated in the confluent monolayer after tumor necrosis factor alpha activation. PMID: 24690766
- E-selectin interactions with glycoprotein ligands (CD44/hematopoietic cell E-/L-selectin ligand and PSGL-1) mediate the initial capturing of cells out of flow. PMID: 26124272
- SELE rs3917412 and MTHFR rs1801133 SNPs could serve as pharmacogenetic predictors of tumor recurrence in patients with early-stage colon cancer treated with oxaliplatin-based adjuvant chemotherapy PMID: 24980946
- sE-selectin and the LDL were the most important predictors of late arteriovenous fistula stenosis PMID: 24627030
- The C allele of E-selectin A561C gene polymorphism might increase the essential hypertension (EH) risk in Asian population, whereas the T allele of E-selectin C1839T gene polymorphism might decrease the EH risk. [meta-analysis] PMID: 25003340
- Bereaved respondents had higher levels of inflammatory biomarkers IL-6 and sE-selectin, but not CRP and sICAM-I than the non-bereaved. PMID: 24266503
- SE-selectin and PAPPA may contribute to future cardiovascular events in women post preeclampsia; in contrast, ApoB may be involved in a protective mechanism PMID: 25238218
- the haplotype TCC in the Han hypertensive patients and the haplotype GGC in the Mongolian patients had independent effects in increasing the risk for essential hypertension; multiple single-nucleotide polymorphisms in combination in SELE may confer a risk of hypertension. PMID: 25748737
- Found the AC genotype to be a significant risk factor for hemorrhagic stroke and we also found significant differences in AC genotype and C allele among hemorrhagic stroke and ischemic stroke patients. PMID: 25168603
- The rs5361 E-selectin gene polymorphism is not a susceptibility marker for ACS in Western Mexico population. However, sE-selectin may be a biological marker of ACS. PMID: 25147432
- ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes PMID: 25498912
- an upregulation of E-selectin and the associated influx of neutrophils may play a significant role in the resolution of inflammation as well as for the pathophysiology of nasal polyps of Caucasian chronic rhinosinusitis patients. PMID: 24995349
- in human aortic and vein endothelial cells, expression and release of ICAM-1 and E-selectin were induced by treatment with conditioned medium collected from Pal-stimulated pTHP-1 cells PMID: 25201247
- CD62E was found to be a new biomarker for patients with hypertension and hyperlipidemia. PMID: 24820735
- High E-Selectin expression is associated with small cell lung cancer metastasis. PMID: 24699516
- there was association between the E-selectin S128R polymorphism and the risk of cancer. PMID: 24815478
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亞細胞定位:Cell membrane; Single-pass type I membrane protein.
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蛋白家族:Selectin/LECAM family
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