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  4. Human E3 ubiquitin-protein ligase MYLIP(MYLIP) ELISA kit

Human E3 ubiquitin-protein ligase MYLIP(MYLIP) ELISA kit

  • 中文名稱：
    人E3泛素蛋白連接酶MYLIP(MYLIP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL015319HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人E3泛素蛋白連接酶MYLIP(MYLIP)酶聯(lián)免疫試劑盒（CSB-EL015319HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的MYLIP含量。MYLIP 是一個關(guān)鍵靶點。它廣泛參與細胞內(nèi)脂質(zhì)代謝調(diào)控等過程。研究表明，MYLIP 可影響相關(guān)脂蛋白的表達與代謝，其機制涉及與特定蛋白相互作用等。對它的深入研究有助于理解脂質(zhì)代謝紊亂疾病，也為開發(fā)針對性治療手段提供方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，本產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域中對MYLIP蛋白表達量的動態(tài)監(jiān)測，例如探究藥物干預(yù)、基因編輯或疾病模型中MYLIP的功能變化，為脂代謝信號通路研究、動脈硬化機制分析及神經(jīng)退行性疾病相關(guān)實驗提供可靠的定量工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BM-023 ELISA Kit; BZF1 ELISA Kit; E3 ubiquitin protein ligase MYLIP ELISA Kit; E3 ubiquitin-protein ligase MYLIP ELISA Kit; Idol ELISA Kit; Inducible degrader of the LDL-receptor ELISA Kit; Lipoprotein receptor ELISA Kit; MIR ELISA Kit; MYLIP ELISA Kit; MYLIP_HUMAN ELISA Kit; Myosin regulatory light chain interacting protein ELISA Kit; PP5242 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MYLIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 105    
    Range % 100-110    
    1:2 Average % 96    
    Range % 92-99    
    1:4 Average % 89    
    Range % 84-94    
    1:8 Average % 93    
    Range % 90-96    
  • 回收率：
    The recovery of human MYLIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 101 99-103    
    EDTA plasma (n=4) 90 85-95    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.778 2.864 2.821 2.689    
    500 2.270 2.305 2.288 2.156    
    250 1.520 1.532 1.526 1.394    
    125 0.919 0.956 0.938 0.806    
    62.5 0.554 0.524 0.539 0.407    
    31.2 0.408 0.398 0.403 0.271    
    15.6 0.263 0.258 0.261 0.129    
    0 0.129 0.134 0.132 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
  • 基因功能參考文獻：
    1. the effects caused by human inducible degrader of the low-density lipoprotein expression are LDLR- dependent given the unchanged plasma lipids in LAhB mice lacking low-density lipoprotein receptor PMID: 26786161
    2. The long noncoding RNA RP1-13D10.2 may contribute to LDL cholesterol levels in response to statins. PMID: 27071970
    3. Specifically, loss of IDOL increases LDLR distribution in the hepatic cell, and subsequently reduces serum LDL-C levels in dyslipidemic patients PMID: 26601593
    4. Data suggest inducible expression of IDOL is subject to robust, rapid regulation by process that is sensitive to deubiquitinase inhibition in human/mouse cell lines and primary human cells; transcriptional induction of IDOL leads to degradation of LDLR. PMID: 26719329
    5. Identify USP2 as a novel regulator of lipoprotein clearance owing to its ability to control ubiquitylation-dependent degradation of the LDLR by IDOL. PMID: 26666640
    6. The study identified MARCH6 as a negative regulator of SREBP2-mediated transcription and described an unexpected E3 circuit functionally linking MARCH6 and IDOL to limit uptake of low-density lipoprotein via the LDLR pathway. PMID: 26527619
    7. IDOL N342S Variant, Atherosclerosis Progression and Cardiovascular Disorders in the Italian General Population. PMID: 25927920
    8. Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels. PMID: 23109900
    9. study indicates that MYLIP p.N342S might be a pharmacogenetic marker for lipid-lowering therapy in patients with FH. PMID: 25171759
    10. Liver-specific expression of dominant-active IDOL is associated with hypercholesterolemia and a marked elevation in atherosclerotic lesions in transgenic mice. PMID: 24935961
    11. Results show that IDOL contributes to variation in circulating levels of LDL-C. PMID: 23324548
    12. IDOL is recruited to plasma membrane by low-density lipoprotein receptor (LDLR), promotes LDLR internalization in the absence of clathrin or caveolae, and facilitates LDLR degradation by shuttling it into the multivesicular body protein-sorting pathway PMID: 23382078
    13. MYLIP rs3757354 SNP is associated with serum TC, HDL-C and ApoAI levels in the Bai Ku Yao and Han populations. But the association is different between the two ethnic groups. PMID: 23107276
    14. No association of the MYLIP rs9370867 genotypes with lipid profile, hemodynamic data, and coronary angiographic data was in a Brazilian population. PMID: 22741812
    15. FGF21 also enhanced expression of Canopy2 (Cnpy2)/MIR-interacting Saposin-like protein (Msap) that is known to interact with Mylip/Idol. PMID: 22378787
    16. expression levels rise with increasing age in hearts of men PMID: 22261164
    17. both the FERM and RING domains are required for promoting lysosomal degradation of the LDLR by IDOL. PMID: 21734303
    18. N342S MYLIP polymorphism is associated with high total cholesterol and increased LDL receptor degradation in humans PMID: 21765216
    19. identify the IDOL-UBE2D complex as an important determinant of LDLR activity, and provide insight into molecular mechanisms underlying the regulation of cholesterol uptake PMID: 21685362
    20. Data report that statins exert opposite effects on PCSK9 and Idol gene expression in human hepatoma-derived cell lines and primary hepatocytes isolated from hamsters and rats. PMID: 21069265
    21. MSAP interacts with this protein that enhances neurite outgrowth and increases myosin regulatory light chain in fetal and adult brain. PMID: 12826659
    22. c-MIR is the first example of an E3 ubiquitin ligase that is capable of inhibiting major histocompatibility (MHC) class II expression in antigen-presenting cells; c-MIR might potently regulate immune responses in vivo. PMID: 16785530
    23. study shows the LXR-Idol(Mylip)-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake PMID: 19520913
    24. Novel insights into the physiology of this receptor come from studies on the ubiquitination and degradation of LDL receptor by the ubiquitin ligase Mylip/Idol that is induced in cells by the nuclear receptor, LXR. PMID: 19688294

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  • 亞細胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein.
  • 組織特異性：
    Ubiquitously expressed.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 21155

    OMIM: 610082

    KEGG: hsa:29116

    STRING: 9606.ENSP00000349298

    UniGene: Hs.484738