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  4. Human EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit

Human EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit

  • 中文名稱：
    人EGF樣重復(fù)序列和盤狀結(jié)構(gòu)域蛋白3(EDIL3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL007399HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人EGF樣重復(fù)序列和盤狀結(jié)構(gòu)域蛋白3(EDIL3)酶聯(lián)免疫試劑盒（CSB-EL007399HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EDIL3含量。EDIL3，全稱EGF-like repeats and discoidin I-like domains 3，是一種細(xì)胞外基質(zhì)蛋白，在胚胎發(fā)育過程中由內(nèi)皮細(xì)胞分泌，并在多種腫瘤細(xì)胞中表達(dá)。它通過其RGD模體結(jié)合整合素αvβ3，促進(jìn)血管生成和抗炎作用，同時(shí)其盤狀結(jié)構(gòu)域可識別磷脂酰絲氨酸，協(xié)助吞噬凋亡細(xì)胞和清除血小板微粒。在腫瘤微環(huán)境中，EDIL3的異常表達(dá)可能與腫瘤生長、血管生成和轉(zhuǎn)移有關(guān)。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域，如探究 EDIL3 在腫瘤微環(huán)境、心血管疾病模型或炎癥相關(guān)疾病中的表達(dá)調(diào)控機(jī)制，也可用于體外藥物干預(yù)或基因編輯后靶蛋白水平的動(dòng)態(tài)評估，為分子機(jī)制研究提供可靠工具。試劑盒操作便捷，配套標(biāo)準(zhǔn)品及預(yù)包被板條，兼容常規(guī)實(shí)驗(yàn)室檢測流程，適用于細(xì)胞生物學(xué)、病理學(xué)等領(lǐng)域的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    DEL1 ELISA Kit; Developmental endothelial locus 1 ELISA Kit; Developmentally regulated endothelial cell locus 1 protein ELISA Kit; Developmentally-regulated endothelial cell locus 1 protein ELISA Kit; Edil3 ELISA Kit; EDIL3_HUMAN ELISA Kit; EGF like repeat and discoidin I like domain containing protein 3 ELISA Kit; EGF like repeats and discoidin I like domains 3 ELISA Kit; EGF like repeats and discoidin I like domains protein 3 ELISA Kit; EGF-like repeat and discoidin I-like domain-containing protein 3 ELISA Kit; Integrin binding protein DEL1 ELISA Kit; Integrin-binding protein DEL1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EDIL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %80-95
    1:2Average %95
    Range %89-100
    1:4Average %99
    Range %91-110
    1:8Average %94
    Range %88-98
  • 回收率：
    The recovery of human EDIL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9280-98
    EDTA plasma (n=4)9692-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.033 1.872 1.953 1.765
    40001.598 1.476 1.537 1.349
    20001.077 1.014 1.046 0.858
    10000.774 0.729 0.752 0.564
    5000.513 0.494 0.504 0.316
    2500.374 0.359 0.367 0.179
    1250.291 0.278 0.285 0.097
    00.190 0.186 0.188 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
  • 基因功能參考文獻(xiàn)：
    1. EDIL3 may be implicated in retinal neovascularization in vitro. Silencing EDIL3 expression was demonstrated to impair the proliferative, migratory and tube forming capabilities of HRECs in vitro. PMID: 28765888
    2. these data reveal an unrecognised function of endogenous Del-1 as a local inhibitor of ischaemia-induced angiogenesis by restraining LFA-1-dependent homing of pro-angiogenic haematopoietic cells to ischaemic tissues PMID: 28447099
    3. Del-1-induced HSC proliferation and myeloid lineage commitment were mediated by beta3 integrin on hematopoietic progenitors. PMID: 28846069
    4. Del-1 pre-treatment of blood before addition of islets diminished coagulation activation and islet damage PMID: 26676803
    5. Del-1 on circulating EVs is a promising marker to improve identification of patients with early-stage breast cancer and distinguish breast cancer from benign breast tumors and noncancerous diseases. PMID: 26603257
    6. Overexpressed EDIL3 promotes anchorage-independent tumor growth in human pancreatic cancer. PMID: 26735172
    7. Overexpression of the Del-1 gene potentiates proliferation and invasion of lung carcinoma cells. PMID: 26545781
    8. The importance of interaction between EDIL3 and integrin alphaV. PMID: 25273699
    9. The reciprocal regulation between Del-1 and inflammation may contribute to optimally balance the protective and the potentially harmful effects of inflammatory cell recruitment. PMID: 24416060
    10. Downregulation of developmentally regulated endothelial cell locus-1 inhibits the growth of colon cancer. PMID: 19292890
    11. Del-1 may act as a gatekeeper of adrenal gland inflammation and may regulate the integrity of the hypothalamic-pituitary-adrenal axis stress response, thereby modulating adrenal (dys)function in the course of SIRS. PMID: 23364949
    12. database search for EGF domain sequences shows that this RGD finger is likely an evolutionary insertion and unique to the EGF domain of Del-1 PMID: 22601780
    13. Del-1 mediates phosphatidylserine- and integrin-dependent endothelial uptake of microparticles by endocytosis. PMID: 22388320
    14. High expression level of EDIL3 predicts poor prognosis of hepatocellular carcinoma patients. PMID: 20857535
    15. Del1 mediates vascular smooth muscle cell adhesion, migration, and proliferation through interaction with integrin alpha(v)beta(3). PMID: 11959660
    16. acts as an angiogenic factor in the context of solid tumor formation; the increase in vascularization accelerates tumor growth through decreased apoptosis PMID: 12074641
    17. Alpha v beta 5, alpha v beta 5 and their ligands Del-1 and L1 play an important role in the process of tumor cells moving from the original place. PMID: 18090124

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  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3173

    OMIM: 606018

    KEGG: hsa:10085

    STRING: 9606.ENSP00000296591

    UniGene: Hs.482730