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  4. Human Ecto-NOX disulfide-thiol exchanger 2(ENOX2) ELISA kit

Human Ecto-NOX disulfide-thiol exchanger 2(ENOX2) ELISA kit

  • 中文名稱：
    人外NOx硫硫醇交換器2(ENOX2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL007677HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人外NOx硫硫醇交換器2(ENOX2)酶聯(lián)免疫試劑盒（CSB-EL007677HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的ENOX2含量。ENOX2是一種編碼細(xì)胞表面NADH氧化酶的ECTO-NOX基因家族的腫瘤特異性成員，具有兩種酶活性：對(duì)苯二酚或NADH氧化的催化，以及蛋白質(zhì)二硫化物交換。研究顯示，ENOX2抑制可增強(qiáng)效應(yīng)記憶T細(xì)胞的浸潤(rùn)并介導(dǎo)對(duì)化療的反應(yīng)，可能成為增強(qiáng)化療效果的有前景的治療策略。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于探索ENOX2在腫瘤發(fā)生、代謝性疾病或氧化應(yīng)激相關(guān)機(jī)制研究中的表達(dá)規(guī)律，如通過(guò)檢測(cè)體外細(xì)胞模型、動(dòng)物實(shí)驗(yàn)組織或臨床前生物樣本中ENOX2的動(dòng)態(tài)變化，為分子機(jī)制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    APK1 ELISA Kit; APK1 antigen ELISA Kit; COVA1 ELISA Kit; Cytosolic ovarian carcinoma antigen 1 ELISA Kit; Ecto NOX disulfide thiol exchanger 2 ELISA Kit; ENOX 2 ELISA Kit; ENOX2 ELISA Kit; ENOX2_HUMAN ELISA Kit; OTTHUMP00000024021 ELISA Kit; OTTHUMP00000024022 ELISA Kit; Protein disulfide-thiol oxidoreductase ELISA Kit; tNOX ELISA Kit; Tumor associated hydroquinone oxidase ELISA Kit; Tumor-associated hydroquinone oxidase ELISA Kit
  • 縮寫：
    ENOX2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ENOX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 82-97  
    1:2 Average % 98  
    Range % 92-104  
    1:4 Average % 102  
    Range % 96-108  
    1:8 Average % 89  
    Range % 82-95  
  • 回收率：
    The recovery of human ENOX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 88 82-92  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.314 2.428 2.371 2.213  
    1000 1.497 1.586 1.542 1.384  
    500 0.937 0.882 0.910 0.752  
    250 0.567 0.553 0.560 0.402  
    125 0.365 0.376 0.371 0.213  
    62.5 0.271 0.264 0.268 0.110  
    31.25 0.218 0.214 0.216 0.058  
    0 0.157 0.158 0.158    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
  • 基因功能參考文獻(xiàn)：
    1. The tumor-associated NADH oxidase (tNOX)-mediated modulation of the NAD+ concentration and SIRT1 are involved in oxaliplatin-induced apoptosis. PMID: 28122359
    2. These findings not only shed light on the molecular mechanism of the anticancer properties of capsaicin, but also the transcription regulation of tNOX expression that may potentially explain how POU3F2 is associated with tumorigenesis. PMID: 27271588
    3. The findings support a role for arNOX as a major source of oxidative damage leading to cross-linking of skin proteins. PMID: 24906676
    4. The results suggest that phosphorylation of serine-504 by PKCdelta modulates the biological function of tNOX. PMID: 22659163
    5. this result suggests that hnRNP F directs formation of the exon 4 minus variant of ENOX2. PMID: 21625959
    6. increased NADH levels resulting from ENOX2 inhibition result in decreased prosurvival sphingosine-1-phosphate and increased proapoptotic ceramide, both of which may be important to initiation of the ENOX2 inhibitor-induced apoptotic cascade. PMID: 21571040
    7. ENOX2 is a dimeric protein containing 4 coppers/dimer capable of carrying out concerted four electron transfers from NADH or ubiquinol to molecular oxygen as required to form water. PMID: 20922471
    8. arNOX activity correlates with age and reaches a maximum at about age 65 in males and 55 in females. PMID: 20345278
    9. Enhanced arNOX activity correlates with age and with oxidative changes contributing to skin aging. PMID: 19734125
    10. tNOX is both necessary and sufficient for the cellular anticancer activities attributed to both EGCg and capsaicin. *tNOX enzyme PMID: 15706060
    11. results indicate that shRNA targeting of tNOX inhibits the growth of cervical cancer cells, and reduces cell migration via a decrease in the membrane association of Rac. PMID: 18023414
    12. A relationship of tNOX to unregulated growth of cancer cells was provided by data where growth of HeLa cells was inhibited by transfection with the exon 5 antisense oligonucleotides PMID: 18351130
    13. These results indicate that tNOX is suppressed during apoptosis and demonstrate that tNOX down-regulation sensitizes cells to stress-induced growth reduction, suggesting that tNOX is required for transformed cell growth. PMID: 18789934

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  • 亞細(xì)胞定位：
    Cell membrane. Secreted, extracellular space. Note=Extracellular and plasma membrane-associated.
  • 蛋白家族：
    ENOX family
  • 組織特異性：
    Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed in
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2259

    OMIM: 300282

    KEGG: hsa:10495

    STRING: 9606.ENSP00000337146

    UniGene: Hs.171458