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  4. Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3(ENPP3) ELISA kit

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3(ENPP3) ELISA kit

  • 中文名稱(chēng)：
    人膜外核苷酸焦磷酸酶/磷酸二酯酶家族成員3(ENPP3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL007681HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人膜外核苷酸焦磷酸酶/磷酸二酯酶家族成員3(ENPP3)酶聯(lián)免疫試劑盒（CSB-EL007681HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的ENPP3含量。ENPP3 作為一個(gè)靶點(diǎn)，在眾多疾病研究中備受關(guān)注。其背景與細(xì)胞信號(hào)傳導(dǎo)、腫瘤微環(huán)境等密切相關(guān)。研究機(jī)制方面，它參與調(diào)節(jié)細(xì)胞內(nèi)外的核苷酸代謝，還可能影響腫瘤細(xì)胞的增殖、遷移等過(guò)程，為相關(guān)疾病的治療提供了重要潛在靶點(diǎn)。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于基礎(chǔ)科研中ENPP3蛋白表達(dá)的動(dòng)態(tài)監(jiān)測(cè)，如探究其在腫瘤微環(huán)境、免疫調(diào)節(jié)或代謝性疾病模型中的功能機(jī)制，也可用于細(xì)胞實(shí)驗(yàn)、動(dòng)物模型或生物樣本庫(kù)中ENPP3水平的系統(tǒng)性比較研究，為分子機(jī)制探索提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Alkaline phosphodiesterase I ELISA Kit; ARHR2 ELISA Kit; B10 ELISA Kit; CD203c ELISA Kit; CD203c antigen ELISA Kit; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; E NPP 3 ELISA Kit; E-NPP 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 ELISA Kit; ENPP3 ELISA Kit; ENPP3_HUMAN ELISA Kit; gp130RB13 6 ELISA Kit; gp130RB136 ELISA Kit; M6S1 ELISA Kit; NPP1 ELISA Kit; NPP3 ELISA Kit; NPPase ELISA Kit; NPPS ELISA Kit; Nucleotide pyrophosphatase ELISA Kit; PC 1 ELISA Kit; PC-1 ELISA Kit; PCA1 ELISA Kit; PD Ibeta ELISA Kit; PD-Ibeta ELISA Kit; PDNP1 ELISA Kit; PDNP3 ELISA Kit; Phosphodiesterase I beta ELISA Kit; Phosphodiesterase I/nucleotide pyrophosphatase 3 ELISA Kit; phosphodiesterase i/nucleotide pyrophosphatase beta ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ENPP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 93-101
    1:2 Average % 99
    Range % 92-105
    1:4 Average % 89
    Range % 83-94
    1:8 Average % 91
    Range % 87-96
  • 回收率：
    The recovery of human ENPP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 98-109
    EDTA plasma (n=4) 85 80-90
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.755 2.858 2.807 2.691
    5 2.212 2.221 2.217 2.101
    2.5 1.537 1.477 1.507 1.391
    1.25 0.946 0.954 0.950 0.834
    0.625 0.489 0.473 0.481 0.365
    0.312 0.289 0.274 0.282 0.166
    0.156 0.210 0.218 0.214 0.098
    0 0.115 0.116 0.116 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has also alkaline phosphodiesterase activity.
  • 基因功能參考文獻(xiàn)：
    1. We showed the ability of the immunological marker CD203c to predict the clinical efficacy of sublingual immunotherapy on rhinitis subjects allergic to Parietaria. PMID: 29702283
    2. Basophil CD203c surface expression reliably discriminated cystic fibrosis with allergic bronchopulmonary aspergillosis from cystic fibrosis with Aspergillus colonization and cystic fibrosis over time. PMID: 26585435
    3. Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy. PMID: 23581640
    4. Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils. PMID: 24497482
    5. ENPP3 is a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) PMID: 23960081
    6. The early signaling requirements for the CD11b/CD203c compartment expression and CD63 degranulation provide support for the hypothesis that CD11b and CD203c reside in a similar compartment. PMID: 22722613
    7. Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen PMID: 20975283
    8. Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission PMID: 20159259
    9. Influence of hyperosmotic conditions on basophil CD203c upregulation in patients with food-dependent exercise-induced anaphylaxis. PMID: 20047266
    10. Data show that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils. PMID: 19418203
    11. E-NPP3 is associated with carcinogenesis of human colon cancer and that serum E-NPP3 might be a tumor marker of colon carcinoma PMID: 14533006
    12. E-NPP3 is involved in the infiltration of neoplastic bile duct carcinoma PMID: 15072822
    13. Our data demonstrate that leptin promotes platelet activation, provides a mechanistic basis for the prothrombotic effect of this hormone, and identifies a potentially novel therapeutic avenue to limit obesity-associated cardiovascular disease. PMID: 15886225
    14. In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID: 17275019

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Secreted.
  • 蛋白家族：
    Nucleotide pyrophosphatase/phosphodiesterase family
  • 組織特異性：
    Detected on bile ducts in liver, and in blood serum (at protein level). Detected in prostate and uterus. Detected on basophils, but not neutrophils.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 3358

    OMIM: 602182

    KEGG: hsa:5169

    STRING: 9606.ENSP00000350265

    UniGene: Hs.486489