Human Elafin(PI3) ELISA kit
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中文名稱:人彈性蛋白酶抑制劑(PI3)酶聯(lián)免疫試劑盒
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貨號:CSB-EL017952HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人彈性蛋白酶抑制劑(PI3)酶聯(lián)免疫試劑盒(CSB-EL017952HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PI3含量。PI3信號通路是一種細胞內(nèi)信號轉(zhuǎn)導途徑,響應細胞外信號,調(diào)節(jié)多種細胞功能,如代謝、增殖、細胞存活、生長和血管生成等。其研究機制涉及PI3K(磷脂酰肌醇3-激酶)和AKT(蛋白激酶B)等關鍵基因,通過磷酸化下游靶標調(diào)控細胞生長、存活和凋亡等生命活動。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL,靈敏度為1.95 pg/mL。該產(chǎn)品廣泛應用于基礎醫(yī)學研究領域,如炎癥相關信號通路解析、蛋白酶 - 抗蛋白酶系統(tǒng)平衡機制探索,以及疾病模型中 PI3 動態(tài)變化的定量分析,為呼吸系統(tǒng)疾病、免疫調(diào)節(jié)及組織損傷修復等方向的科研工作提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Cementoin ELISA Kit; ELAF_HUMAN ELISA Kit; Elafin ELISA Kit; Elafin/Skalp ELISA Kit; Elastase Specific Inhibitor ELISA Kit; Elastase-specific inhibitor ELISA Kit; ES 1 ELISA Kit; ESI ELISA Kit; Peptidase inhibitor 3 ELISA Kit; Peptidase inhibitor 3; skin derived ELISA Kit; PI 3 ELISA Kit; PI-3 ELISA Kit; PI3 ELISA Kit; Pre elafin ELISA Kit; Protease inhibitor 3 skin derived ELISA Kit; Protease inhibitor 3; skin derived (SKALP) ELISA Kit; Protease inhibitor WAP3 ELISA Kit; SKALP ELISA Kit; Skin derived Anti leukoproteinase ELISA Kit; Skin-derived antileukoproteinase ELISA Kit; Trappin 2 ELISA Kit; WAP four disulfide core domain 14 ELISA Kit; WAP four disulfide core domain protein 14 ELISA Kit; WAP four-disulfide core domain protein 14 ELISA Kit; WAP3 ELISA Kit; WFDC14 ELISA Kit
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縮寫:PI3
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:7.8 pg/mL-500 pg/mL
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靈敏度:1.95 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
62.974
63.991
62.090
61.964
63.482
61.586
SD
0.042
0.035
0.037
0.052
0.047
0.050
CV(%)
5.364
4.425
4.768
6.710
5.972
6.477
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Elafin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:20
Average %
89
Range %
85-93
1:40
Average %
100
Range %
95-105
1:80
Average %
87
Range %
83-91
1:160
Average %
105
Range %
101-109
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回收率:
The recovery of human Elafin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range%
Serum (n=5)
86
83-89
EDTA plasma (n=4)
99
95-104
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標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
500
2.783
2.901
2.842
2.678
250
1.852
1.798
1.825
1.661
125
1.210
1.205
1.208
1.044
62.5
0.779
0.789
0.784
0.620
31.2
0.483
0.478
0.481
0.317
15.6
0.331
0.354
0.343
0.179
7.8
0.279
0.268
0.274
0.110
0
0.165
0.163
0.164
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數(shù)據(jù)處理:
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貨期:3-5 working days
相關產(chǎn)品
靶點詳情
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功能:Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1).
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基因功能參考文獻:
- Elafin decreased in active patients with inflammatory bowel disease and was negatively correlated with disease activity, suggesting that elafin may play a protective role PMID: 29084078
- Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome PMID: 28187039
- UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
- Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia PMID: 28802561
- significantly higher expression in preterm prelabor ruptured fetal membranes than in membranes from term birth PMID: 27580179
- Our study revealed that high elafin levels identified in smoking- and asthma-related microarray data sets and an epidemiologic study significantly reduced the risk of asthma. PMID: 26932165
- The expression of elafin in fallopian tubes of ectopic pregnancies is reduced. PMID: 25356943
- The elevations in cervical elafin and SLPI expression in the women with preterm delivery might reflect the local response to the pathogen invasion into the cervix preceding preterm labor. PMID: 25559229
- PI3 expression was downregulated in 24% of DCIS and 83% of invasive breast tumors. PI3 was also downregulated in 33% of ovarian cystadenomas, 43% of borderline ovarian tumors, and 86% of invasive ovarian carcinomas. PMID: 25551582
- The imbalance between neutrophil elastase and its inhibitors, such as elafin, presents an important therapeutic target in breast cancer. PMID: 25195861
- Higher urinary elafin levels are associated with an increased risk of micro- and macroalbuminuria, acute kidney injury and chronic kidney disease, and death after hematopoietic stem cell transplantation. PMID: 25388519
- cutaneous elafin does not seem to discriminate acute graft-versus-host disease from drug hypersensitivity rash lesions, but there may be an association between cutaneous elafin expression and poor prognosis for patients with cutaneous GVHD PMID: 25405322
- Data indicate that elafin overexpression is associated with poor overall survival. PMID: 24469047
- Low expression of elafin gene correlated with significantly reduced time to relapse, and when combined with high expression of elastase gene was associated with decreased survival in breast cancer patients PMID: 23320734
- Elafin has important roles in normal homoeostasis and at sites of inflammation. These roles include antiprotease and antimicrobial activity. PMID: 19906197
- Results confirm the association between SNP rs2664581 and enhanced risk of developing acute respiratory distress syndrome. PMID: 24617927
- Tubal epithelium from women with hydrosalpinx has lower levels of elafin expression. PMID: 23885101
- Elafin expression in the small intestinal epithelium was lower in patients with active celiac disease compared with control patients. In vitro, elafin significantly slowed the kinetics of the deamidation of the 33-mer peptide to its more immunogenic form. PMID: 24710505
- Production of HBD2 and elafin by vaginal epithelial cells is sensitive to E, suggesting that innate immune protection varies in the vagina across the menstrual cycle. PMID: 23398087
- Elafin expression may establish the diagnosis of pre-engraftment syndrome and graft versus host disease. PMID: 23866880
- Trappin-2 and elafin significantly reduce HSV-2 viral attachment to, and replication in genital epithelial cells. PMID: 23637403
- The N-terminus of elafin is critical for intranuclear localization and anti-HIV-1 activity. PMID: 23300756
- Studies indicate that Trappin-2/Elafin possessing anti-microbial properties against different classes of pathogens including viruses, fungi and bacteria. PMID: 22634606
- The results suggest that decreased expression of elastin and LOX and increased expression of elafin in the cardinal ligaments may contribute to pelvic organ prolapse. PMID: 19087518
- The endometrium of women with hydrosalpinx has an increased number of neutrophils and lower expression of elafin, an elastase inhibitor and natural antimicrobial molecule. PMID: 21392745
- these results collectively suggest that, in melanoma cells, Foxa2 expression is silenced and therefore elafin is maintained unexpressed to facilitate cell proliferation in the disease melanoma. PMID: 21466784
- ability to modulate the expression of some P.aeruginosa virulence factors PMID: 20932308
- These results suggest that elafin can maintain airway epithelium integrity by protecting airway epithelial cells and enhancing the anti-inflammatory capability of airway. PMID: 19823954
- increased production at the secretory phase of the menstrual cycle relative to the proliferative phase PMID: 19824918
- Elafin has a role in graft-versus-host disease of the skin PMID: 20371463
- Elafin may serve as a determinant of poor survival in serous ovarian carcinomas PMID: 20126474
- Overexpression of elafin in epithelial cells attenuated the damage of Pseudomonas aeruginosa biofilm. PMID: 19420899
- Elafin is a component of cervicovaginal secretions in pregnancy, and levels are diminished in bacterial vaginosis. PMID: 19723838
- Synthesized locally at mucosal sites. Efficiently inhibit target enzymes released into interstitium. Might be important in controlling excessive neutrophil elastase release in chronic obstructive pulmonary disease and cystic fibrosis. (review) PMID: 11667971
- regulation of the elafin gene is dependent on chromatin structure in keratinocytes PMID: 12542536
- results indicate that elafin may have a dual function, promoting up-regulation of local lung innate immunity while simultaneously down-regulating potentially unwanted systemic inflammatory responses in the circulation PMID: 12819058
- elafin regulates proteolytic enzymes during menstruation and contributes to the innate defense against uterine infection PMID: 12970320
- Serine elastase inhibition (elafin overexpression) appears to suppress inflammation, cardiac dilatation, and dysfunction after myocardial infarct. PMID: 14693682
- Gene augmentation of human elafin suggests a novel and extended anti-inflammatory role for this human neutrophil elastase inhibitor working as an effector of innate immunity to protect tissues against maladaptive inflammatory responses. PMID: 15034071
- The differential biological activity of elafin in different conditions suggests a role for this molecule in either LPS detoxification or activation of innate immune responses, depending on the external cellular environment. PMID: 15668324
- Oxidized elafin poorly inhibits the elastolytic activity of leukocyte elastase and proteinase 3. PMID: 16279952
- transglutaminase 2-mediated cross-linking of serine protease inhibitors elafin and trappin-2 to neutrophil serine proteinases preserves their inhibitory capacities PMID: 16300411
- The pleiotropic molecule elafin has significant potential in modulating antigen-presenting cell numbers and activity, and could be beneficial in mucosal protective strategies. PMID: 16424380
- The promoter sequences of PI3 have a high degree of variability. PMID: 16719916
- beneficial effects of pre-elafin could be mediated, at least in part, by its ability to increase G-CSF levels in the lung PMID: 16913840
- The transglutaminase substrate moiety of elafin plays an important role in anchoring elafin at its proper sites of action during UV-induced aging processes. PMID: 17139263
- Levels of mRNA and immunostaining of the antiproteases elafin and SLPI were enhanced strongly in inflamed versus noninflamed UC. Elafin and SLPI may be added to the list of defensin-like peptides with diminished induction in CD versus UC. PMID: 17200145
- full antipeptidase activity of pre-elafin is essential to protect against lung tissue lesions PMID: 17489739
- This review focuses on recent findings revealing that elafin has many biological functions as diverse as anti-bacterial, anti-fungal, anti-viral, anti-inflammatory and immuno-modulatory functions. PMID: 17964057
- We report here that pre-elafin inhibits a secreted peptidase to prevent Pseudomonas aeruginosa growth in vitro. PMID: 18025118
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亞細胞定位:Secreted.
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