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  4. Human Enteropeptidase(PRSS7) ELISA kit

Human Enteropeptidase(PRSS7) ELISA kit

  • 中文名稱：
    人腸肽酶(PRSS7)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL018824HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人腸肽酶(PRSS7)酶聯(lián)免疫試劑盒（CSB-EL018824HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PRSS7含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品適用于基礎(chǔ)科研中探索PRSS7在胃腸功能調(diào)控、營(yíng)養(yǎng)代謝相關(guān)實(shí)驗(yàn)?zāi)Ｐ椭械谋磉_(dá)動(dòng)態(tài)，或用于評(píng)估特定干預(yù)措施對(duì)腸道蛋白酶活性的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    TMPRSS15; ENTK; PRSS7; Enteropeptidase; Enterokinase; Serine protease 7; Transmembrane protease serine 15
  • 縮寫(xiě)：
    PRSS7
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %100
    Range %95-110
    1:2Average %90
    Range %85-85
    1:4Average %92
    Range %88-98
    1:8Average %98
    Range %93-104
  • 回收率：
    The recovery of human PRSS7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-93
    EDTA plasma (n=4)9388-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    202.528 2.422 2.475 2.358
    101.746 1.692 1.719 1.602
    51.164 1.110 1.137 1.020
    2.50.654 0.649 0.652 0.535
    1.250.413 0.434 0.424 0.307
    0.6250.271 0.253 0.262 0.145
    0.3120.195 0.208 0.202 0.085
    00.120 0.114 0.117
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • 基因功能參考文獻(xiàn)：
    1. The Y174R variant showed improved specificities for substrates containing the sequences DDDDK (kcat/KM = 6.83 x 106 M-1 sec-1) and DDDDR (kcat/KM = 1.89 x 107 M-1 sec-1) relative to all other enteropeptidase variants reported to date. PMID: 23436726
    2. Enteropeptidase is a gene associated with a starvation human phenotype and a novel target for obesity treatment PMID: 23185382
    3. Human enteropeptidase shows 10x faster kinetics compared to other animal sources but low solubility under low salt conditions. A supercharged variant of enteropeptidase light chain with increased solubility was used for crystallization. PMID: 22488687
    4. Characterization of the different catalytic activity of human and bovine enteropeptidase light chains toward hydrolysis of peptides and proteins lacking tetraaspartate sequence. PMID: 22571433
    5. Engineered recombinant enteropeptidase catalytic subunit: effect of N-terminal modification PMID: 11913964
    6. Produced in enterocytes and goblet cells. Localization on brush border of cells for physiological activation of digestive enzymes. In duodenal polyps and adenocarcinoma at duodenum but not in Brunner's gland adenoma. PMID: 12907431
    7. Enterokinase directly cleaved proMMP-9 at the Lys65-Ser66 site. PMID: 18062964

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  • 相關(guān)疾病：
    Enterokinase deficiency (ENTKD)
  • 亞細(xì)胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Intestinal brush border.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 9490

    OMIM: 226200

    KEGG: hsa:5651

    STRING: 9606.ENSP00000284885

    UniGene: Hs.149473