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  4. Human Fetuin-B(FETUB) ELISA kit

Human Fetuin-B(FETUB) ELISA kit

  • 中文名稱：
    人胎球蛋白B(FETUB)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL008598HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人胎球蛋白B(FETUB)酶聯(lián)免疫試劑盒（CSB-EL008598HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FETUB含量。試劑盒檢測(cè)范圍為0.137 ng/ml-100 ng/ml，靈敏度為0.137 ng/ml。適用于科研領(lǐng)域?qū)ρ濉⒀獫{及組織勻漿樣本中FETUB蛋白含量的精準(zhǔn)分析；為研究者探索FETUB在代謝調(diào)控、肝臟病理機(jī)制中的生物學(xué)功能提供可靠工具；用于相關(guān)疾病模型或干預(yù)實(shí)驗(yàn)中生物標(biāo)志物的動(dòng)態(tài)監(jiān)測(cè)；適用于細(xì)胞實(shí)驗(yàn)、動(dòng)物模型研究及人類樣本分析等科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    16G2 ELISA Kit; 2310011O17Rik ELISA Kit; AI255764 ELISA Kit; D17980 ELISA Kit; Fet ELISA Kit; Fetub ELISA Kit; FETUB_HUMAN ELISA Kit; Fetuin beta ELISA Kit; Fetuin-B ELISA Kit; Fetuin-like protein ELISA Kit; Fetuin-like protein IRL685 ELISA Kit; Gugu ELISA Kit; IRL685 ELISA Kit; Pp63 ELISA Kit
  • 縮寫：
    FETUB
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.137 ng/ml-100 ng/ml.
  • 靈敏度：
    0.137 ng/ml.
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    12.720

    13.079

    12.874

    12.643

    12.823

    12.925

    SD

    0.023

    0.027

    0.025

    0.036

    0.033

    0.031

    CV(%)

    3.622

    4.160

    3.900

    5.696

    5.164

    4.821

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Fetuin B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:10

    Average %

    106

    Range %

    102-108

    1:20

    Average %

    88

    Range %

    84-92

    1:40

    Average %

    92

    Range %

    85-96

    1:80

    Average %

    87

    Range %

    82-83

  • 回收率：

    The recovery of human Fetuin B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    104

    98-108

    EDTA plasma (n=4)

    98

    94-102

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    2.775

    2.859

    2.817

    2.665

    33.33

    1.951

    1.937

    1.944

    1.792

    11.11

    1.090

    1.062

    1.076

    0.924

    3.7

    0.642

    0.691

    0.666

    0.514

    1.23

    0.385

    0.364

    0.375

    0.223

    0.41

    0.283

    0.285

    0.284

    0.132

    0.137

    0.218

    0.235

    0.226

    0.074

    0

    0.151

    0.153

    0.152

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening.
  • 基因功能參考文獻(xiàn)：
    1. High Serum fetuin-B expression is associated with insulin resistance. PMID: 29194974
    2. fetuin B may serve as a potential biomarker for coronary artery disease PMID: 28822077
    3. Increased serum fetuin-B level was significantly associated with higher risk of insulin resistance. Common genetic variants of FETUB SNPs (rs4686434, rs6785067, and rs3733159) were significantly associated with serum fetuin-B concentrations but were not associated with insulin resistance. PMID: 29390354
    4. serum level of fetuin B in the control subjects was lower than it in nonalcoholic fatty liver disease patients. fetuin B correlated with diastolic blood pressure, serum alanine transaminase and triglycerides, among the controls. It suggested a potential association between serum fetuin B and the presence of NAFLD. PMID: 27628583
    5. Further studies need to elucidate factors contributing to fetuin B regulation in humans, as well as the pathophysiological significance of fetuin B upregulation in gestational diabetes PMID: 28183456
    6. Data indicate that fetuin-B was one of the highly expressed serum proteins in acute myocardial infarction. PMID: 25671698
    7. Reduced expression of fetuin-b led to a significant increase in the expression of lipogenic genes, thereby resulting in higher lipid levels. PMID: 22739111
    8. serum fetuin has a role in processing and transport of matrix gamma-carboxyglutamic acid protein and bone morphogenetic protein-2 in cultured human vascular smooth muscle cells PMID: 15280384
    9. results support the hypothesis that fetuin-A serum levels are associated with bone cell activity PMID: 16797392

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Fetuin family
  • 組織特異性：
    Liver and testis.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 3658

    OMIM: 605954

    KEGG: hsa:26998

    STRING: 9606.ENSP00000265029

    UniGene: Hs.81073