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  4. Human Fibrinogen-like protein 1(FGL1) ELISA kit

Human Fibrinogen-like protein 1(FGL1) ELISA kit

  • 中文名稱：
    人纖維蛋白原樣蛋白1(FGL1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL008653HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人纖維蛋白原樣蛋白1(FGL1)酶聯(lián)免疫試劑盒（CSB-EL008653HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGL1含量。FGL1是新興免疫治療靶點。它可作為LAG-3的配體，在腫瘤免疫逃逸中發(fā)揮重要作用。阻斷FGL1與LAG-3相互作用，能解除腫瘤微環(huán)境免疫抑制，激活T細胞抗腫瘤活性，目前是腫瘤免疫治療研究熱點。試劑盒檢測范圍為0.156 ng/ml-10 ng/ml.，該產(chǎn)品適用于探索FGL1在腫瘤微環(huán)境中的功能機制、肝臟疾病相關(guān)生物標志物篩選，以及代謝綜合征等基礎(chǔ)研究領(lǐng)域，為免疫調(diào)節(jié)通路研究、疾病模型構(gòu)建及藥物作用靶點驗證提供有效工具。實驗操作兼容常規(guī)ELISA流程，支持科研人員開展體外樣本的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FGL 1 ELISA Kit; Fgl1 ELISA Kit; FGL1_HUMAN ELISA Kit; Fibrinogen like 1 ELISA Kit; Fibrinogen like protein 1 ELISA Kit; Fibrinogen related protein 1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Hepassocin ELISA Kit; Hepatocellular carcinoma related sequence ELISA Kit; Hepatocyte derived fibrinogen related protein 1 ELISA Kit; Hepatocyte-derived fibrinogen-related protein 1 ELISA Kit; HFREP 1 ELISA Kit; HFREP-1 ELISA Kit; HFREP1 ELISA Kit; HP 041 ELISA Kit; HP-041 ELISA Kit; HP041 ELISA Kit; LFIRE 1 ELISA Kit; LFIRE-1 ELISA Kit; LFIRE1 ELISA Kit; Liver fibrinogen related protein 1 ELISA Kit; Liver fibrinogen-related protein 1 ELISA Kit; MFIRE 1 ELISA Kit; MGC108569 ELISA Kit; MGC12455 ELISA Kit; MGC37822 ELISA Kit; OTTHUMP00000122468 ELISA Kit
  • 縮寫：
    FGL1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/ml-10 ng/ml.
  • 靈敏度：
    0.039 ng/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of Human FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    84-94

    1:2

    Average %

    87

    Range %

    82-93

    1:4

    Average %

    95

    Range %

    89-99

    1:8

    Average %

    98

    Range %

    92-104

  • 回收率：

    The recovery of Human FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    88-97

    EDTA plasma (n=4)

    87

    82-92

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.758

    2.645

    2.702

    2.607

    5

    2.107

    2.256

    2.182

    2.087

    2.5

    1.601

    1.524

    1.563

    1.468

    1.25

    0.867

    0.845

    0.856

    0.761

    0.625

    0.475

    0.483

    0.479

    0.384

    0.312

    0.317

    0.304

    0.311

    0.216

    0.156

    0.181

    0.176

    0.179

    0.084

    0

    0.095

    0.094

    0.095

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human FGL1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled FGL1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：人

稀釋比：其他 1：10

產(chǎn)品評價： 我用CSB-EL008653HU檢測正常和疾病組血清，原始OD值為0.1-40，兩組結(jié)果具有顯著差異，用WB驗證與之結(jié)果一致，認為該結(jié)果可信。該試劑盒質(zhì)量好，靈敏度高。

By 魏老師

相關(guān)問答

■ 常見問題解答
Q:

我們想使用你們的FGL1 Elisa Kit CSB-EL008653HU。不幸的是，我們的血漿樣本中混入了檸檬酸鈉。但是說明書上只提到EDTA或肝素，我是否可以用這個試劑盒檢測我們的樣本？

A:
原則上，如果試劑不影響蛋白質(zhì)結(jié)構(gòu)變化(包括蛋白質(zhì)變性)，則可以使用該試劑。但過酸或過堿的試劑會影響蛋白質(zhì)。 我們一般是直接制備血漿檢測，不使用其他試劑(如乙酸鈉)，所以無法判斷會影響檢測結(jié)果。如果您的樣品尚未準備好，建議按照試劑盒的說明操作。如果已經(jīng)準備好了，請安排一次預(yù)實驗來檢查結(jié)果。

靶點詳情

  • 功能：
    Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes.
  • 基因功能參考文獻：
    1. FGL1 promotes invasion and metastasis of gastric cancer and is associated with poor prognosis. PMID: 29845203
    2. findings highlight the crucial role of HFREP1 in insulin resistance and diabetes, and provide a potential strategy and biomarker for developing therapeutic approaches to combat these diseases. PMID: 27221093
    3. Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
    4. Here, the application of small ubiquitin-related modifier (SUMO) fusion technology in combination with four different chaperone teams on the soluble expression of recombinant HPS protein was explored and analyzed. PMID: 24084006
    5. Findings suggest that hepassocin promotes hepatic cell line L02 cells proliferation via an autocrine mechanism and inhibits HCC cells proliferation by an intracrine pathway. PMID: 21618590
    6. data demonstrated that liver-specific gene LFIRE-1/HFREP-1 was frequently downregulated and might possess growth suppressor activity in HCC PMID: 14981537
    7. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation PMID: 16996032
    8. The enhancement of FGL1 levels in vitro by IL-6 and its induction after turpentine oil injection suggest that it is an acute phase reactant. PMID: 18039467
    9. HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666

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  • 亞細胞定位：
    Secreted.
  • 組織特異性：
    Under normal conditions, liver-specific.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3695

    OMIM: 605776

    KEGG: hsa:2267

    STRING: 9606.ENSP00000221204

    UniGene: Hs.491143