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  4. Human Fibroblast growth factor 17(FGF17) ELISA kit

Human Fibroblast growth factor 17(FGF17) ELISA kit

  • 中文名稱：
    人成纖維細(xì)胞生長(zhǎng)因子17(FGF17)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL008622HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人成纖維細(xì)胞生長(zhǎng)因子17(FGF17)酶聯(lián)免疫試劑盒（CSB-EL008622HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液.樣本中的FGF17含量。FGF17是一種成纖維細(xì)胞生長(zhǎng)因子，在胚胎發(fā)育等過(guò)程中發(fā)揮重要作用。其研究機(jī)制聚焦于它與受體結(jié)合后激活下游信號(hào)通路，調(diào)控細(xì)胞增殖、分化等。在腫瘤等疾病中可能參與發(fā)生發(fā)展，為相關(guān)治療提供潛在靶點(diǎn)。試劑盒檢測(cè)范圍為3.12 pg/ml-200 pg/ml.，研究者可將其應(yīng)用于發(fā)育生物學(xué)、疾病機(jī)制研究或藥物干預(yù)實(shí)驗(yàn)中FGF17表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)，為探索其生理功能及病理意義提供可靠的工具支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    FGF 13 ELISA Kit; FGF 17 ELISA Kit; FGF-17 ELISA Kit; FGF13 ELISA Kit; Fgf17 ELISA Kit; FGF17_HUMAN ELISA Kit; Fibroblast growth factor 17 ELISA Kit; HH20 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates.
  • 檢測(cè)范圍：
    3.12 pg/ml-200 pg/ml.
  • 靈敏度：
    0.78 pg/ml.
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FGF17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    90

    Range %

    85-96

    1:2

    Average %

    95

    Range %

    88-101

    1:4

    Average %

    100

    Range %

    93-105

    1:8

    Average %

    93

    Range %

    86-101

  • 回收率：

    The recovery of human FGF17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    86-100

    EDTA plasma (n=4)

    97

    92-103

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    200

    2.264

    2.234

    2.249

    2.078

    100

    1.582

    1.667

    1.625

    1.454

    50

    0.986

    1.028

    1.007

    0.836

    25

    0.660

    0.629

    0.645

    0.474

    12.5

    0.453

    0.431

    0.442

    0.271

    6.25

    0.338

    0.343

    0.341

    0.170

    3.12

    0.251

    0.244

    0.247

    0.077

    0

    0.166

    0.175

    0.171

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
  • 基因功能參考文獻(xiàn)：
    1. FGF17 and IL17RD prpopsed as the two top candidates in the entire proteome on the basis of a statistical test of their protein-protein interaction patterns to proteins known to be altered in congenital hypogonadotropic hypogonadism. PMID: 23643382
    2. FGF17 expression is increased 2-fold in benign prostatic hyperplasia and may contribute to the increased epithelial proliferation seen in this disease. PMID: 15129425
  • 相關(guān)疾病：
    Hypogonadotropic hypogonadism 20 with or without anosmia (HH20)
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 組織特異性：
    Preferentially expressed in the embryonic brain.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 3673

    OMIM: 603725

    KEGG: hsa:8822

    STRING: 9606.ENSP00000352414

    UniGene: Hs.248192