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  4. Human Fibulin-1(FBLN1) ELISA kit

Human Fibulin-1(FBLN1) ELISA kit

  • 中文名稱：
    人腓骨蛋白1(FBLN1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL008452HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人腓骨蛋白1(FBLN1)酶聯(lián)免疫試劑盒（CSB-EL008452HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FBLN1含量。FBLN1（fibulin-1）是一種分泌性糖蛋白，參與細胞外基質(zhì)構(gòu)建，與層粘連蛋白和巢蛋白結(jié)合，介導(dǎo)血小板粘附。研究顯示，F(xiàn)BLN1在動脈粥樣硬化、食管癌等疾病中表達增加，其表達受microRNA調(diào)控，可能通過影響細胞增殖、遷移等途徑參與疾病發(fā)生發(fā)展。試劑盒檢測范圍為6.25 ug/ml-400 ug/ml，靈敏度為1.56 ug/ml。適用于科研領(lǐng)域中對FBLN1表達調(diào)控的機制研究、疾病模型中的蛋白動態(tài)分析以及生物樣本中FBLN1含量的定量檢測。為探索FBLN1在組織微環(huán)境重塑、疾病標志物篩選等方向提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Basement membrane protein 90 ELISA Kit; BM 90 ELISA Kit; FBLN ELISA Kit; Fbln1 ELISA Kit; FBLN1_HUMAN ELISA Kit; FIBL 1 ELISA Kit; FIBL-1 ELISA Kit; FIBL1 ELISA Kit; Fibulin 1 ELISA Kit; Fibulin-1 ELISA Kit
  • 縮寫：
    FBLN1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ug/ml-400 ug/ml
  • 靈敏度：
    1.56 ug/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    93

    Range %

    90-96

    1:2

    Average %

    86

    Range %

    82-95

    1:4

    Average %

    93

    Range %

    89-98

    1:8

    Average %

    96

    Range %

    92-101

  • 回收率：

    The recovery of human FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    101

    96-106

    EDTA plasma (n=4)

    93

    88-99

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    μg/ml

    OD1

    OD2

    Average

    Corrected

    400

    2.698

    2.556

    2.627

    2.497

    200

    1.667

    1.540

    1.604

    1.474

    100

    0.953

    0.970

    0.962

    0.832

    50

    0.576

    0.573

    0.575

    0.445

    25

    0.449

    0.458

    0.454

    0.324

    12.5

    0.351

    0.353

    0.352

    0.222

    6.25

    0.252

    0.260

    0.256

    0.126

    0

    0.131

    0.129

    0.130

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

Hi,?has?this?kit?been?tested?for?cross-reactivity?with?other?fibulin?isoforms?and?with?fibronectin?

A:
Thanks for your inquiry!
fibulin includes FBLN1, FBLN2, FBLN3, FBLN4, FBLN5, FBLN7 and HMCN1 is also known as "fibulin-6".
There is across activity between the recombinant protein for CSB-EL008452HU and another isoforms.

靶點詳情

  • 最新研究進展：
    FBLN1是一種與細胞外基質(zhì)相互作用的蛋白，已被研究與多種疾病的發(fā)病機制有關(guān)，如心血管疾病、癌癥、肝病等。近期研究發(fā)現(xiàn)FBLN1還參與了免疫炎癥反應(yīng)的調(diào)節(jié)，可以抑制單核細胞的活化和炎癥細胞的浸潤，為探索新的治療策略提供了新思路。
  • 功能：
    Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
  • 基因功能參考文獻：
    1. FBLN1 and STK31 can be possible screening and differentiating biomarkers of colorectal cancer PMID: 29715435
    2. Fibulin-1 is expressed in breast cancer cell lines, interacts wtih SHBG, and is increased by estradiol PMID: 29940241
    3. This study demonstrated that FBLN1 can effectively promote osteogenic differentiation of human nasal inferior turbinate-derived mesenchymal stem cells into osteoblasts. PMID: 28445604
    4. This study demonstrated that fibulin-1 (FBLN1) was detected in the serums of Alzheimer disease patients PMID: 27911324
    5. Interestingly and in contrast with our expectation, we found that the expression level of FBLN-4 and BCRP were downregulated in tumor compared to adjacent normal tissues. FBLN-4 was associated with grade histology and therefore can be considered as a potential prognostic biomarker. PMID: 28282800
    6. Serum and vitreous fibulin-1 concentrations are elevated under diabetic retinopathy condition. PMID: 27422995
    7. Galectin-3 and fibulin-1 levels are elevated in heart failure patients with impaired glucose metabolism. Reduced LV contractile reserve among HF patients with DM does not to have an independent impact on plasma Gal-3 and fibulin-1 levels. PMID: 28068900
    8. changes in methylation levels in cfDNA associated with hepatocellular carcinoma and could represent useful plasma-based biomarker PMID: 28333958
    9. The FBLN1/FGF8 interaction may also be involved in the survival of neural crest cell population during development. PMID: 27402846
    10. Low expression of fibulin-1 is associated with gastric cancer. PMID: 26779638
    11. LDH associated positively with fibulin-1 (beta = 0.23; p < 0.001) and ROS (beta = 0.11; p = 0.30) in blacks only with cardiovascular disease. PMID: 26631026
    12. The procedure described is the first to our knowledge that enables a large scale purification of Fibulin-1 from human plasma. PMID: 26826315
    13. Study identifies a novel fibulin-1D variant, named fibulin-1D', comprising 638 amino acids with molecular weight of 70.5 kDa. Fibulin-1D' lacks a secretion sequence, has a truncated N-terminus domain and binds to the intracellular domain of integrin beta1. PMID: 25661773
    14. The purpose of this study was to develop and characterize robust quantitative assays for the emerging cardiovascular biomarker fibulin-1 and its circulating isoforms in human plasma. PMID: 25331251
    15. Extracellular matrix biomarker, fibulin-1 and its association with soluble uPAR in a bi-ethnic South African population PMID: 25456503
    16. Treatment with low-dose spironolactone reduced plasma fibulin-1 levels in patients with type 2 diabetes and resistant hypertension. PMID: 24739800
    17. fibulin-1 expression is associated with NMIBC grade and recurrence, it is epigenetically down-regulated and functions as a tumor suppressor gene and angiogenesis inhibitor in bladder cancer. PMID: 25234557
    18. Plasma fibulin-1 is independently associated with pulse wave velocity in type 2 diabetics. PMID: 23866070
    19. Increased levels of fibulin-1 were independently associated with higher levels of suPAR and NT-proBNP especially in patients with lower aortic valve stenosis PMID: 25014213
    20. Mutation of fibulin-1 causes a novel syndrome involving the central nervous system and connective tissues. PMID: 24084572
    21. FBLN1 functions as a novel candidate tumor suppressor gene in CM, and its downregulation may be due to promoter hypermethylation. PMID: 23907575
    22. Increased plasma fibulin-1 levels are associated with diabetes and impaired kidney function. PMID: 23294625
    23. Fibulin-1 is down regulated in renal cell carcinoma through promoter hypermethylation. PMID: 23391467
    24. In single regression analysis, NT-proBNP was significantly associated with fibulin-1 in African men and Caucasian women. PMID: 22349089
    25. Fibulin-1 is down-regulated in atrial tissue from patients with atrial fibrillation, perhaps reflecting the myocardial structural changes that may have taken place during arrhythmia. PMID: 20451270
    26. plasma levels of fibulin-1 could serve as a potential indicator to monitor kidney malfunction or kidney damage PMID: 21888404
    27. Fibulin-1 accumulates in the arterial wall and in plasma of patients with type 2 diabetes, and appears to be a factor associated with arterial extracellular matrix changes in type 2 diabetes. PMID: 21926180
    28. Promoter hypermethylation of FBLN1 was significantly associated with advanced stage hepatocellular carcinoma, multiple tumors and increased tumor size. PMID: 21268132
    29. data collectively suggest fibulin-1C may be worthy of further investigation as a target for airway remodeling in asthma PMID: 20967215
    30. Fibulin-1 protein was expressed in guinea pig sclera and cultured human scleral fibroblasts. PMID: 20405022
    31. the pattern of fibulin-1 within human atherosclerotic lesions and the potential for fibulin-1, perhaps derived from the blood and acting in conjunction with fibrinogen, to play a role in the etiology and cardiovascular disease progression PMID: 19693531
    32. Fibulin-1 has pronounced inhibitory effects on cell attachment and spreading promoted by fibronectin (FN) and suppresses the motility of a variety of cell types on FN substrates. PMID: 11792823
    33. structural and functional characterization of promoter PMID: 11829738
    34. FBLN1 disrupted by a t(12;22) translocation is associated with a complex type of synpolydactyly. PMID: 11836357
    35. binding of HPV E6 protein to fibulin-1 PMID: 12200142
    36. findings indicate that elevated expression and altered processing of fibulin-1 is associated with human breast cancer PMID: 12644824
    37. The gene responsible for a unique vitreoretinal dystrophy is located on chromosome 22q13. PMID: 12912698
    38. mutations in the splice acceptor site of fibulin-1 exon 19, but no MYH9 mutations,were found in family individuals affected by autosomal-dominant giant platelet syndromes associated with aberrant antisense gene regulation of the fibulin-1 gene. PMID: 14635206
    39. FBLN seems to have an immunological and pathobiological role in breast neoplasm surveillance. PMID: 14691454
    40. The preferential induction of the fibulin-1C variant, which is overexpressed in ovarian and breast cancer, might play an important role in estrogen-promoted carcinogenesis. PMID: 15528301
    41. fiblin-1 is an important molecule that mediates progesterone action in human endometrial stromal cells differentiation towards implantation PMID: 15774544
    42. fibulin-1 is a new regulator of ADAMTS-1-mediated proteoglycan proteolysis and may play an important role in proteoglycan turnover in tissues where there is overlapping expression PMID: 16061471
    43. Coexpression of fibulin-1 with GROalpha abrogates key aspects of the transformed phenotype, including colonic tumor formation in a murine xenograft model. PMID: 17062666
    44. degradation of basement membrane by cathepsin D liberates both fibulin-1 fragments and fibulin-5, which function to inhibit angiogenesis PMID: 18222970
    45. FBLN1 was identified as a novel candidate tmour suppressor gene epigenetically downregulated in gastric cancer PMID: 18985039
    46. Like tenascin-C, fibulin-1 inhibits fibroblast spreading and cell-mediated contraction of a fibrin-fibronectin matrix. PMID: 19109427
    47. fibulin-1 and -2 respond differentially to single and repeated damaging noxae, and their expression is differently present in liver cells PMID: 19609566

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  • 相關(guān)疾?。?/div>
    A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with RASSF8. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation.
  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibulin family
  • 組織特異性：
    Isoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3600

    OMIM: 135820

    KEGG: hsa:2192

    STRING: 9606.ENSP00000331544

    UniGene: Hs.24601