Human Fibulin-7(FBLN7) ELISA kit
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中文名稱:人腓骨蛋白-7(FBLN7)酶聯(lián)免疫試劑盒
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貨號:CSB-EL008455HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人腓骨蛋白-7(FBLN7)酶聯(lián)免疫試劑盒(CSB-EL008455HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的FBLN7含量。FBLN7,即纖連蛋白7,是一種胞外基質(zhì)蛋白,參與多種蛋白間的相互作用。研究發(fā)現(xiàn),F(xiàn)BLN7在心血管發(fā)育、牙齒發(fā)育、腫瘤轉(zhuǎn)移等過程中發(fā)揮重要作用。其研究機(jī)制涉及調(diào)節(jié)細(xì)胞粘附、生長、遷移和存活等細(xì)胞功能。試劑盒檢測范圍為25 pg/mL-1600 pg/mL,適用于基礎(chǔ)科研中FBLN7的蛋白表達(dá)分析,例如探究其在組織修復(fù)、胚胎發(fā)育或特定病理模型(如纖維化疾病、腫瘤微環(huán)境)中的動態(tài)變化,為體外實驗或動物模型研究提供高效工具。通過兼容多種生物樣本類型,研究者可靈活應(yīng)用于體液檢測或組織水平的分子機(jī)制探索,為細(xì)胞外基質(zhì)相關(guān)研究提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:FBLN7 ELISA Kit; FBLN7_HUMAN ELISA Kit; FIBL-7 ELISA Kit; Fibl7 ELISA Kit; Fibulin-7 ELISA Kit; TM14 ELISA Kit
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縮寫:FBLN7
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:25 pg/mL-1600 pg/mL
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靈敏度:6.25 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 87-99 1:2 Average % 95 Range % 87-100 1:4 Average % 92 Range % 87-96 1:8 Average % 97 Range % 91-101 -
回收率:
The recovery of human FBLN7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-97 EDTA plasma (n=4) 91 84-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1600 2.435 2.322 2.379 2.287 800 1.803 1.746 1.775 1.683 400 1.162 1.123 1.143 1.051 200 0.549 0.535 0.542 0.450 100 0.332 0.314 0.323 0.231 50 0.208 0.203 0.206 0.114 25 0.114 0.108 0.111 0.019 0 0.093 0.091 0.092 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點詳情
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功能:An adhesion molecule that interacts with extracellular matrix molecules in developing teeth and may play important roles in differentiation and maintenance of odontoblasts as well as in dentin formation.
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基因功能參考文獻(xiàn):
- Fbln7-C terminal fragment inhibited the HUVEC tube formation and the vessel sprouting in aortic ring assays. PMID: 24480309
- Dysregulation of fibulin expression by anti-Ro/SSA antibodiess may contribute to disorganization of the extracellular environment and thus cause injury to the salivary gland architecture and functionality observed in Sjogren syndrome PMID: 19229767
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亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix.
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蛋白家族:Fibulin family
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數(shù)據(jù)庫鏈接:
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