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  4. Human Follistatin-related protein 5(FSTL5) ELISA kit

Human Follistatin-related protein 5(FSTL5) ELISA kit

  • 中文名稱：
    人卵泡抑素相關(guān)蛋白5(FSTL5)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009028HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人卵泡抑素相關(guān)蛋白5(FSTL5)酶聯(lián)免疫試劑盒（CSB-EL009028HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FSTL5含量。FSTL5是一種鈣離子結(jié)合蛋白，可能參與細(xì)胞分化和細(xì)胞外區(qū)域定位。研究發(fā)現(xiàn)，F(xiàn)STL5與青少年痛風(fēng)新風(fēng)險基因位點(diǎn)相關(guān)，可能通過影響炎癥過程參與痛風(fēng)的發(fā)生。此外，F(xiàn)STL5還與肌肉生長、脂肪棕色化及激素調(diào)節(jié)等功能有關(guān)。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，適用于基礎(chǔ)醫(yī)學(xué)研究中對FSTL5蛋白表達(dá)水平的定量分析，例如探索其在生殖生理調(diào)控、組織修復(fù)機(jī)制或疾病模型中的分子作用?？蒲腥藛T可通過高效檢測生物體液（血清/血漿）中的循環(huán)蛋白含量，或分析特定組織勻漿中FSTL5的局部表達(dá)特征，為研究其生物學(xué)功能及病理相關(guān)性提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FSTL5 ELISA Kit; KIAA1263Follistatin-related protein 5 ELISA Kit; Follistatin-like protein 5 ELISA Kit
  • 縮寫：
    FSTL5
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.31 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FSTL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    92

    Range %

    85-99

    1:2

    Average %

    97

    Range %

    90-104

    1:4

    Average %

    102

    Range %

    95-109

    1:8

    Average %

    92

    Range %

    88-97

  • 回收率：

    The recovery of human FSTL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-101

    EDTA plasma (n=4)

    97

    92-106

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    80

    2.848

    2.694

    2.771

    2.619

    40

    2.465

    2.357

    2.411

    2.259

    20

    1.885

    1.868

    1.877

    1.725

    10

    1.260

    1.229

    1.245

    1.093

    5

    0.720

    0.721

    0.721

    0.569

    2.5

    0.515

    0.533

    0.524

    0.372

    1.25

    0.375

    0.384

    0.380

    0.228

    0

    0.149

    0.155

    0.152

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. FSTL5 plays a suppressive role in hepatocellular carcinoma. Down-regulation of FSTL5 could promote growth and survival of HCC cells by activation of Wnt/beta-catenin signaling. PMID: 26045876
    2. FSTL5 expression denoted a dismal prognosis both within and across medulloblastoma subgroups. PMID: 21911727
  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 21386

    KEGG: hsa:56884

    STRING: 9606.ENSP00000305334

    UniGene: Hs.32452