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  4. Human Galanin-like peptide(GALP) ELISA kit

Human Galanin-like peptide(GALP) ELISA kit

  • 中文名稱：
    人甘丙肽樣肽(GALP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009222HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人甘丙肽樣肽(GALP)酶聯(lián)免疫試劑盒（CSB-EL009222HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GALP含量。GALP（甘丙肽樣肽）是一種神經(jīng)肽。其背景上，它在中樞神經(jīng)系統(tǒng)等有表達(dá)。研究機(jī)制方面，GALP與甘丙肽受體相互作用，參與調(diào)節(jié)能量代謝、生殖功能、攝食行為等生理過程，在生物體內(nèi)發(fā)揮著重要的神經(jīng)調(diào)節(jié)作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，為體外實驗研究其生理病理機(jī)制提供可靠工具，尤其適用于探究代謝調(diào)控、神經(jīng)內(nèi)分泌網(wǎng)絡(luò)及相關(guān)疾病模型的分子機(jī)制，滿足基礎(chǔ)科研中對GALP表達(dá)水平動態(tài)監(jiān)測的需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GALP ELISA Kit; Galanin-like peptide ELISA Kit
  • 縮寫：
    GALP
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %87-97
    1:2Average %101
    Range %97-103
    1:4Average %95
    Range %88-98
    1:8Average %99
    Range %94-102
  • 回收率：
    The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-102
    EDTA plasma (n=4)9488-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.057 2.080 2.069 1.918
    51.761 1.812 1.787 1.636
    2.51.403 1.373 1.388 1.237
    1.250.988 0.973 0.981 0.830
    0.6250.703 0.727 0.715 0.564
    0.3120.477 0.497 0.487 0.336
    0.1560.322 0.331 0.327 0.176
    00.150 0.152 0.151
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2. Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing.
  • 基因功能參考文獻(xiàn)：
    1. results indicated that obese individuals have higher plasma GAL and GALP concentrations and both peptide concentrations were positively correlative to triglyceride concentrations in obese human PMID: 27474259
    2. One isoform (alarin) of this gene but not the other (galanin-like peptide) displays antimicrobial activity against E. coli. PMID: 23537644
    3. This review presents considerable evidence that has accumulated implicating both galanin and GALP as playing important roles in regulating food and water intake behavior and related neuroendocrine functions. PMID: 16787232
    4. describes cloning of the full-length human GALP PMID: 10601261
    5. describes the gene structure of human GALP. PMID: 11861493
    6. reports immunostaining of human GALP in the dermal microvasculature. PMID: 17535903

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Galanin family
  • 組織特異性：
    Isoform 2 is found in ganglia of ganglioneuroma and ganglioneuroblastoma, as well as in differentiated tumor cells of neuroblastoma tissues. Not found in undifferentiated neuroblasts. Isoform 2 is found in the skin, in pericytes covering microvascular art
  • 數(shù)據(jù)庫鏈接：

    HGNC: 24840

    OMIM: 611178

    KEGG: hsa:85569

    STRING: 9606.ENSP00000349884

    UniGene: Hs.283915