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  4. Human Galectin 7(Gal-7) ELISA Kit

Human Galectin 7(Gal-7) ELISA Kit

  • 中文名稱：
    人半乳糖凝集素-7（Gal-7)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13454h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人半乳糖凝集素-7（Gal-7)酶聯(lián)免疫試劑盒（CSB-E13454h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Gal-7含量。Gal-7，也稱為LGALS7，是一種參與細(xì)胞-細(xì)胞和細(xì)胞-基質(zhì)相互作用的蛋白質(zhì)，可能對正常生長控制至關(guān)重要。它作為促凋亡因子，在細(xì)胞內(nèi)發(fā)揮作用，通過JNK激活和細(xì)胞色素c釋放的上游促進(jìn)細(xì)胞凋亡途徑。研究顯示，Gal-7在多種鱗狀上皮癌中異常高表達(dá)，其靶向價(jià)值在癌癥診斷和治療中具有潛在應(yīng)用。試劑盒檢測范圍為78 pg/ml-5000 pg/ml，靈敏度為19.5 pg/ml。該產(chǎn)品適用于科研領(lǐng)域?qū)al-7蛋白表達(dá)水平的精準(zhǔn)分析，例如探究炎癥性疾病、皮膚病理模型或腫瘤微環(huán)境中Gal-7的動(dòng)態(tài)變化規(guī)律，也可用于體外培養(yǎng)細(xì)胞或動(dòng)物實(shí)驗(yàn)樣本的分子機(jī)制研究，為探索Gal-7在免疫調(diào)控、細(xì)胞間信號傳導(dǎo)等生物學(xué)過程中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Gal-7 ELISA Kit; GAL7 ELISA Kit; Galectin 7B ELISA Kit; Galectin-7 ELISA Kit; HKL-14 ELISA Kit; Human keratinocyte lectin 14 ELISA Kit; Keratinocyte lectin 14 ELISA Kit; Lectin galactoside binding soluble 7 ELISA Kit; Lectin; galactoside binding; soluble; 7B ELISA Kit; LEG7_HUMAN ELISA Kit; LGALS7 ELISA Kit; LGALS7A ELISA Kit; LGALS7B ELISA Kit; P53 induced protein 1 ELISA Kit; p53-induced gene 1 protein ELISA Kit; Pi7 ELISA Kit; PIG1 ELISA Kit; TP53I1 ELISA Kit
  • 縮寫：
    Gal-7
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 pg/ml-5000 pg/ml
  • 靈敏度：
    19.5 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    644.292

    616.477

    630.349

    622.774

    649.379

    631.614

    SD

    0.030

    0.035

    0.035

    0.041

    0.048

    0.043

    CV(%)

    4.249

    5.117

    5.036

    5.951

    6.761

    6.178

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Gal-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    91

    Range %

    88-96

    1:2

    Average %

    95

    Range %

    90-102

    1:4

    Average %

    97

    Range %

    94-104

    1:8

    Average %

    101

    Range %

    95-105

  • 回收率：

    The recovery of human Gal-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    87-96

    EDTA plasma (n=4)

    98

    95-103

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.819

    2.910

    2.865

    2.739

    2500

    1.857

    1.830

    1.844

    1.718

    1250

    1.140

    1.057

    1.099

    0.973

    625

    0.750

    0.729

    0.740

    0.614

    312

    0.422

    0.422

    0.422

    0.296

    156

    0.291

    0.311

    0.301

    0.175

    78

    0.183

    0.177

    0.180

    0.054

    0

    0.123

    0.129

    0.126

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
  • 基因功能參考文獻(xiàn)：
    1. Increased O-linked N-Acetyl-glucosamine (O-GlcNAc) is responsible for reduced Gal-7 expression in cultured human keratinocytes exposed to high glucose environment. PMID: 28526214
    2. We observed a significantly reduced overall survival for cases with high Gal-7 expression and a better survival for Gal-7 negative cases, when compared to cases with low expression of Gal-7. PMID: 28594391
    3. Data suggest that evaluation of galectin-7 could help guide postsurgical management for non-metastatic clear cell renal cell carcinoma (ccRCC). PMID: 27259255
    4. these findings reveal the existence of a positive self-amplification pathway that regulates intracellular gal-7 expression in breast and ovarian cancer cells. PMID: 29117220
    5. Gal-7 re-expression affects the regulation of molecular networks in cervical cancer that are involved in diverse cancer hallmarks, such as metabolism, growth control, invasion and evasion of apoptosis PMID: 27558259
    6. Measurement of scGal-7 content in tape-stripped samples was useful for the evaluation of the skin barrier function in dry skin conditions such as AD PMID: 27028525
    7. Data indicate that the galectin inhibitor specifically binds galectin-7 (hGal-7), disrupts the formation of homodimers, and inhibits the pro-apoptotic activity of hGal-7 on Jurkat T cells. PMID: 26543238
    8. Galectin-7 regulates keratinocytes proliferation and differentiation through JNK1 pathway. PMID: 26763438
    9. The assessment of a carbohydrate-recognition domain (CRD)-defective mutant form of gal-7 (R7S) showed that the ability of this protein to modulate apoptosis was independent of its CRD activity. PMID: 26168167
    10. our study validates the clinical significance of gal-7 overexpression in ovarian cancer PMID: 25277199
    11. Cytosolic galectin-7 impairs p53 functions and induces chemoresistance in breast cancer cells. PMID: 25367122
    12. Galectin-7 is produced by the premenstrual and menstrual endometrium, where it accumulates in menstrual fluid and likely acts as a paracrine factor to facilitate post-menstrual endometrial re-epithelialization. PMID: 24782449
    13. Understanding how these dendrimeric compounds interact with hGal-7 would help in the design of new tools to investigate the recognition of carbohydrates by lectins. PMID: 25367374
    14. galectin-7 is a potential predictive marker of chemotherapy and/or radiotherapy resistance in patients with oral squamous cell carcinoma PMID: 24515895
    15. galectin-7 may have a role in amyloidogenesis of primary localized cutaneous amyloidosis PMID: 25172508
    16. C/EBPbeta is an important mediator of galectin-7 gene activation in breast cancer cells PMID: 24789216
    17. Galectin-7 is produced by endometrial epithelium. It is elevated in the endometrium of women with history of miscarriage. We suggest that galectin-7 facilitates adhesion of embryo to endometrium. Elevated galectin-7 may result in abnormal adhesion. PMID: 24522232
    18. galectin-7 may play important roles in downregulating the functions of T lymphocytes after UVB irradiation PMID: 24134186
    19. galectin-7 has a tumor suppressive function, and that the gene is epigenetically modified by DNA methylation and significantly down-regulated in gastric cancer. PMID: 23985992
    20. Suggest that the differences in galectin-7 expression and subcellular and extracellular distribution may be crucially involved in the pathogenic process of hypertrophic scars. PMID: 23493985
    21. galectin-7 could be part of a common pathway used by mutant p53 to promote cancer progression PMID: 23967302
    22. The results indicate that lactose binding to human Gal-7 induces long-range effects (minor conformational shifts and changes in structural dynamics) throughout the protein that result in stabilization of the dimer state, with evidence for positive cooperativity. PMID: 23376190
    23. High GAL-7 expression is associated with epithelial ovarian cancer. PMID: 23564797
    24. Knocking down galectin-7 or S100A9 enhanced tumor cell invasion. PMID: 22864818
    25. Because residual cholesteatoma matrix is considered to be one of the main causes of cholesteatoma recurrence, staining with galectin-7 at the time of operation would be a promising way to facilitate complete removal of the residue PMID: 22377647
    26. Extracellular superoxide dismutase plays a role not only as a reactive oxygen species scavenger, but also as a pro-apoptotic factor via COX-2/galectin-7 pathways in the epidermis. PMID: 22251572
    27. high resolution crystal structure of Gal-7 at 1.4 A (a dimer) and its 1.7 A structure in complex with an inhibitor, a sulfur-containing 2-O-benzylphosphate galactoside, a possible antineoplastic agent PMID: 22059385
    28. Data suggest that the binding of Galectin 7 to Bcl-2 may constitute a new target for enhancing the intrinsic apoptosis pathway. PMID: 21289092
    29. Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells. PMID: 21677144
    30. Data show that high levels of galectin-7 expression in breast cancer cells drastically increased their ability to metastasize to lungs and bones, and were restricted to high-grade breast carcinomas. PMID: 20382700
    31. Proteomics analysis revealed that galectin-7 was highly expressed in esophageal squamous cell carcinoma and could be used as a potential biomarker. PMID: 20546628
    32. Data show that Galectine-7 gene was 8 times up-regulated in bronchial epithelial cells from asthmatic children after RSV infection in vitro and overexpressed in bronchial epithelial cells in asthma in vivo. PMID: 17044979
    33. galectin-7 increases the expression of MMP-9 through the p38 MAPK signaling pathway PMID: 19885589
    34. GAL7 is a negative growth regulator for human neuroblastoma cells. PMID: 13679866
    35. High levels of galectin 7 expression were associated with rapid recurrence rates in hypopharyngeal cancer PMID: 16788763
    36. there was no significant difference in Galectin 7 expression in thyroid tissue in thyroid cancer cases compared to non-cancer cases PMID: 18418527
    37. Gal-7 influence on differentiation in vivo, without evidence for a role in dissemination in head and neck squamous and basal cell carcinomas PMID: 19012243
    38. abnormal expression of galectin-7 in lymphoma cells is not dependent on p53, but is rather associated with DNA hypomethylation. PMID: 19596268

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Secreted. Note=May be secreted by a non-classical secretory pathway.
  • 組織特異性：
    Mainly expressed in stratified squamous epithelium.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6568

    OMIM: 600615

    KEGG: hsa:3963

    STRING: 9606.ENSP00000367891

    UniGene: Hs.558355