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  4. Human Gastrin-releasing peptide,GRP ELISA Kit

Human Gastrin-releasing peptide,GRP ELISA Kit

  • 中文名稱：
    人胃泌素釋放多肽(GRP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09167h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人胃泌素釋放多肽(GRP)酶聯(lián)免疫試劑盒（CSB-E09167h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織培養(yǎng)上清液、組織勻漿樣本中的GRP含量。GRP（胃泌素釋放肽）是一種胃腸激素，廣泛分布于哺乳動物的神經(jīng)系統(tǒng)、胃腸道和呼吸道。其研究機制主要涉及GRP-R（胃泌素釋放肽受體）的拮抗劑，通過調(diào)節(jié)胃酸分泌、胃腸道運動以及某些癌細胞生長等方面發(fā)揮作用。抑制GRP-R可間接調(diào)節(jié)GRP活性，為研究GRP在生理和病理過程中的作用提供了重要工具。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，該產(chǎn)品適用于胃酸分泌調(diào)控機制研究、腫瘤微環(huán)境中神經(jīng)肽功能分析，以及神經(jīng)內(nèi)分泌系統(tǒng)相關(guān)疾病的分子機制探索等科研場景，為體外實驗提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BN ELISA Kit; Bombesin ELISA Kit; GRP ELISA Kit; GRP-10 ELISA Kit; GRP_HUMAN ELISA Kit; GRP10 ELISA Kit; Neuromedin-C ELISA Kit; NeuromedinC ELISA Kit; Pre progastrin releasing peptide ELISA Kit; PreproGRP ELISA Kit; ProGRP ELISA Kit
  • 縮寫：
    GRP
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.312 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %103
    Range %98-110
    1:4Average %92
    Range %88-96
    1:8Average %86
    Range %80-92
  • 回收率：
    The recovery of human GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.694 2.572 2.633 2.512
    402.082 2.105 2.094 1.973
    201.418 1.476 1.447 1.326
    100.891 0.835 0.863 0.742
    50.540 0.572 0.556 0.435
    2.50.350 0.341 0.346 0.225
    1.250.276 0.262 0.269 0.148
    00.124 0.117 0.121
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Stimulates the release of gastrin and other gastrointestinal hormones. Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. Contributes to long-term fear memory, but not normal spatial memory. Contributes to the regulation of food intake.
  • 基因功能參考文獻：
    1. A panel of three tumor markers CYFRA 21.1, HE4 and ProGRP may play a role for discriminating LC from benign lung disease and subtyping as SCLC. PMID: 29729229
    2. Pro-GRP is sensitive for SCLC diagnosis. Since high marker levels are related to high disease burden, pro-GRP may have a negative prognostic significance. Follow-up studies are required to define its role in clinical practice in monitoring responses to treatment and early relapses PMID: 28967066
    3. Altogether, these data show that humanized anti-progastrin antibodies might represent a potential new treatment for K-RAS-mutated colorectal patients, for which there is a crucial unmet medical need PMID: 28600477
    4. Both TRPV1 and GRP are implied in midbrain physiology of importance to neurological and neuropsychiatric disorders. PMID: 27762319
    5. GRP/GRP-R signaling activation contributes to castration-resistant prostate cancer progression PMID: 27542219
    6. progastrin expression may be predictive of aggressive tumour behaviour in patients with colorectal cancer. PMID: 28432443
    7. Thus the combination of favourable in vitro and in vivo properties renders BA1 as more potential antagonist bombesin-peptide for targeting GRP-receptor positive tumor. These properties are encouraging to carry out further experiments for non-invasive receptor targeting potential diagnostinc and therapeutic agent for tumors. PMID: 28088445
    8. serum level not a biomarker in small cell lung cancer PMID: 29145241
    9. pro-gastrin releasing peptide has a role in promoting the cell proliferation and progression in small cell lung cancer PMID: 27639644
    10. Our results suggest that, similar to what happens in neutrophils, gastrin-releasing peptide is a migratory, rather than a proliferative, stimulus, for non-small cell lung carcinoma cells, indicating a putative role for gastrin-releasing peptide and gastrin-releasing peptide receptor in metastasis PMID: 28351312
    11. Serum Pro-GRP was promising biomarker for SCLC diagnosis. PMID: 28230031
    12. High proGRP expression is associated with poor response to chemotherapy in small cell lung cancer. PMID: 26886220
    13. CEA, NSE, CA125 and pro-GRP could serve as biomarkers for SCLC, and CEA and CYFRA21-1 could serve as biomarkers for NSCLC. Pro-GRP, CA125 and CEA were related to the clinical stages of lung cancer PMID: 26560853
    14. Data show that serum neuron-specific enolase, cytokeratin 19 fragment 21-1, pro-gastrin-releasing peptide, squamous cell carcinoma antigen, tissue inhibitor of metalloproteinase-1, and human epididymis protein 4 are not associated with brain metastases. PMID: 26730601
    15. Our results suggest that progastrin inhibits the acquisition of a M2-phenotype in human macrophages. PMID: 24901518
    16. No association of 16 GRP and 7 GRPR variants were found with agoraphobia with/without panic disorder. PMID: 24912045
    17. the role of autophagy in the degradation of gastrin-releasing peptide and subsequent inhibition of angiogenesis PMID: 24108003
    18. High serum proGRP levels are associated with small-cell lung cancer. PMID: 24375395
    19. The Gastrin-releasing peptide(GRP)triggers the growth of HepG2 cells through blocking the ER stress-mediated pathway. PMID: 23379566
    20. GRP silencing decreases anchorage-independent growth, inhibits cell migration and neuroblastoma cell-mediated angiogenesis. PMID: 24039782
    21. GRP-expressing C and A delta fibers that coexpress SP or CGRP makes these neurons pruriceptors. PMID: 23615431
    22. The levels of GRP were upregulated in cells treated with HGF in a dose-dependent manner. HGF-induced expression of Ets-1 and IL-8 was increased more by GRP treatment. PMID: 23924923
    23. inhibits the process of autophagy in vascular endothelial cells, thereby increasing endothelial cell proliferation and tubule formation PMID: 23608754
    24. GRP serum level is higher in patients with pruritus in patients with atopic dermatitis. PMID: 23353988
    25. Tonic stretch of human myometrium increases contractility and stimulates the expression of a known smooth muscle stimulatory agonist, GRP. GRP receptor antagonists attenuate the effect of stretch. PMID: 22411014
    26. Data indicate that progastrin-releasing peptide (proGRP) assays with both time-resolved immunofluorometric assay (TR-IFMA) and Advanced Life Science Institute (ALSI) ELISA showed good clinical validity. PMID: 22399443
    27. Percent changes in serum ProGRP showed better correlation to the sum of the tumor diameters (SOD) and prognostic impact than that of NSE. PMID: 22300752
    28. Results describe the mRNA expression of CRABP1, RERG, and GRP in pituitary adenomas. PMID: 21270509
    29. nonamidated peptides derived from the C terminus of pro-GRP are expressed in significant quantities in colorectal cancer cell lines PMID: 22202166
    30. proGRP is a complementary tumour marker for prognosis and treatment monitoring in patients with neuroendocrine tumour PMID: 21415235
    31. serum-positive non-small-cell lung cancers may have neuroendocrine differentiation PMID: 21529988
    32. Progastrin-releasing peptide cab be used as a marker for quality control in clinical sample processing and storage. PMID: 22261454
    33. Abrogating GRP/GRPR signaling specifically down-regulates HP1(Hsbeta) expression and inhibiting GRPR signaling, or ablating HP1(Hsbeta) expression, increases colon cancer cell invasiveness in vitro. PMID: 21281799
    34. Gastrin-releasing peptide is elevated in prostate neoplasm patients undergoing androgen deprivation therapy and may be involved in the initiation of hormonal escape prostate neoplasms. PMID: 20945407
    35. GRP promotes the growth of HepG2 cells through interaction with GRPR co-expressed in tumor cells, and subsequently activates MAPK/ERK1/2 via EGFR-independent mechanisms. PMID: 20596631
    36. Data indicate that various serum proGRP fragments are promising tools for future investigations on the relationship between fragment distribution and diagnosis and prognosis. PMID: 19907206
    37. GRP/GRP-R play a transient and non-critical role in intestinal development PMID: 11960700
    38. mRNA transcripts are expressed in tumor cells of patients with small cell lung cancer PMID: 12474049
    39. ProGRP is a valuable tumour marker for the detection and monitoring of small cell lung cancer (SCLC) and a good tool for discriminating non-small cell lung cancer (NSCLC) versus SCLC PMID: 12820318
    40. results show that Pro-GRP may be a potential tumor marker for small cell lung carcinoma PMID: 12820319
    41. Mitogenic effects of GRP in head and neck squamous cells are mediated by activation of the epidermal growth factor receptor. PMID: 13679857
    42. GRP is over-expressed in esophageal squamous cell carcinoma, and its over-expression may play a role in such carcinoma development and growth PMID: 14764456
    43. Measuring serum levels in lung cancer patients may be useful in drug therapy monitoring. PMID: 15638385
    44. GRP is a new angiogenic peptide and that its inhibition offers an attractive tool to reduce tumor burden. PMID: 15750618
    45. An increase in GRP binding capacity, as a result of GRP-R overexpression, down-regulates PTEN expression. Inhibition of the tumor suppressor gene PTEN may be an important regulatory mechanism involved in GRP-induced cell proliferation in neuroblastomas. PMID: 15849504
    46. BN/GRP and substance P are involved together with cytokines in the neuroimmunomodulation that occurs in the arthritic joint. PMID: 15899028
    47. results show that TACE undergoes phosphorylation that regulates release of amphiregulin upon GRP treatment; a signaling cascade of GRP-Src-PI3-K-PDK1-TACE-amphiregulin-EGFR with multiple points of interaction, translocation & phosphorylation is suggested PMID: 16641105
    48. First evidence is provided that the calcium-ion/calcineurin/NFAT-linked pathway is involved in bombesin-mediated induction of Cox-2, a gene that promotes colon carcinoma invasiveness. PMID: 16909108
    49. GRP upregulation of ICAM-1 via FAK promotes tumor cell motility and attachment to the extracellular matrix. PMID: 16920698
    50. BN/GRP is produced by non-neuronal cells in the synovial tissue in rheumatoid and osteoarthritis PMID: 18289674

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  • 亞細胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle lumen.
  • 蛋白家族：
    Bombesin/neuromedin-B/ranatensin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4605

    OMIM: 137260

    KEGG: hsa:2922

    STRING: 9606.ENSP00000256857

    UniGene: Hs.153444