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  4. Human Glutathione Reductase(GR)ELISA Kit

Human Glutathione Reductase(GR)ELISA Kit

  • 中文名稱：
    人谷胱甘肽還原酶（GR）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14286h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人谷胱甘肽還原酶（GR）酶聯(lián)免疫試劑盒（CSB-E14286h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GSR含量。GSR即谷胱甘肽還原酶，在生物體內(nèi)負(fù)責(zé)催化氧化型谷胱甘肽還原為還原型谷胱甘肽，維持細(xì)胞內(nèi)抗氧化防御系統(tǒng)。其研究聚焦于作為藥物靶點(diǎn)，針對(duì)GSR開(kāi)發(fā)的藥物可調(diào)節(jié)細(xì)胞氧化還原狀態(tài)，用于治療氧化應(yīng)激相關(guān)疾病如癌癥、神經(jīng)退行性疾病等。試劑盒檢測(cè)范圍為0.625 mU/mL-40 mU/mL，適用于體外研究GR在神經(jīng)退行性疾病、代謝異?；蛐难芗膊〉炔±磉^(guò)程中的表達(dá)水平變化，為氧化應(yīng)激相關(guān)分子機(jī)制探索提供工具支持。組織勻漿兼容性設(shè)計(jì)可滿足離體組織或動(dòng)物模型中GR的活性檢測(cè)需求，為多維度科研實(shí)驗(yàn)提供靈活選擇。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    GLR1 ELISA Kit; GLUR ELISA Kit; Glutathione reductase ELISA Kit; Glutathione reductase mitochondrial ELISA Kit; Glutathione reductase; mitochondrial ELISA Kit; GR ELISA Kit; Gr1 ELISA Kit; GRase ELISA Kit; GRD 1 ELISA Kit; GRD1 ELISA Kit; GSHR_HUMAN ELISA Kit; GSR ELISA Kit; MGC78522 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.625 mU/mL-40 mU/mL
  • 靈敏度：
    0.156 mU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %88-95
    1:2Average %103
    Range %96-107
    1:4Average %92
    Range %85-95
    1:8Average %98
    Range %92-103
  • 回收率：
    The recovery of human GR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)107101-112
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    402.827 2.787 2.807 2.648
    201.871 1.854 1.863 1.704
    101.192 1.168 1.180 1.021
    50.667 0.643 0.655 0.496
    2.50.466 0.455 0.461 0.302
    1.250.310 0.289 0.300 0.141
    0.6250.256 0.246 0.251 0.092
    00.160 0.158 0.159
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Maintains high levels of reduced glutathione in the cytosol.
  • 基因功能參考文獻(xiàn)：
    1. It has been shown that acute aerobic exercise activates Nrf2 in young men, irrespective of training intensity, but that high-intensity exercise demonstrated a greater effect on increasing glutathione reductase activity which could indicate improved redox potential. PMID: 28693341
    2. GSR expression was higher in TMZ-resistant cells than in sensitive cells. GSR silencing in drug-resistant cells improved the sensitivity of cells to TMZ or cisplatin. Over-expression resulted in resistance. GSR partially prevented the oxidative stress caused by L-buthionine -sulfoximine. The action of GSR in drug resistance is associated with the modulation of redox homeostasis. High GSR correlated with lower survival. PMID: 29105080
    3. Plasma glutathione reductase (GR) activity was correlated with erythrocyte GR activity and the erythrocyte reduced glutathione/glutathione disulfide ratio. A decrease in plasma GR activity was associated with an increase in mortality in septic shock. PMID: 26316444
    4. Glutathione reductase reduces mitochondrial protein mitoNEET [2Fe-2S] clusters. PMID: 25645953
    5. The recurrence of benign tumors of mammary gland occurred predominantly in women-carriers of mutant alleles with polymorphism rs8190924 of gene GSR and AA rs3763511of gene DKK4. PMID: 26419038
    6. GSR was the most significant single SNP association with systemic lupus erythematosus in African Americans. PMID: 23637325
    7. The results demonstrate for the first time that glutathione reductase gene polymorphisms are significantly associated with bone mineral density. PMID: 22089180
    8. Up-regulation of CAT and GR activity resulted in an increase in total antioxidant activity in A549 after exposure to B(a)P. PMID: 21417634
    9. Human eye lenses were dissected into discrete regions that were formed at different stages of life and assayed for activity of lactate dehydrogenase (LDH) and a particularly stable enzyme, glutathione reductase (GR). PMID: 20586645
    10. novel glutathione reductase alternative splice variants PMID: 20628807
    11. GR deficiency and drug-induced GR inhibition may protect from malaria by inducing enhanced ring stage phagocytosis rather than by impairing parasite growth directly [case study] PMID: 19806191
    12. Malignant lung tumors (squamous cell carcinoma and adenocarcinoma) had increased activity of this enzyme. PMID: 12447480
    13. activities of catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were decreased during intense physical exercise PMID: 12516882
    14. An oxidative stress marker after kidney transplantation PMID: 12826156
    15. Patients with chronic alcoholism (PCA) of stage II have increased glutathione reductase (GR) activity in neutrophils, lymphocytes and monocytes compared with that in the cells of healthy donors PMID: 12838767
    16. GR activity in COPD and chest osteomyelitis decreased by 40% and more, lung tumors--by 32-36%. PMID: 12838770
    17. serum concentrations in patients with chronic lymphocytic leukemia PMID: 14637279
    18. Decreased activities of erythrocyte glutathione reductase is associated with cerebral palsy PMID: 15978628
    19. Data show that both glutathione and glutathione reductase are inducible by D3T, and that upregulation of GSH biosynthesis underlies D3T-mediated cytoprotection against ROS/RNS-elicited injury to human vascular smooth muscle cells. PMID: 17206382
    20. Inhibitory effects of several antibiotics on purified human erythrocyte glutathione reductase has been investigated. PMID: 18341267
    21. Data report synchrotron-based cryocrystallographic studies of natural substrate complexes of the flavoenzyme human glutathione reductase at nominal resolutions between 1.1 and 0.95 A that reveal new aspects of its mechanism. PMID: 18638483
    22. higher glutathione reductase and total antioxidant capacity were found in end-stage renal disease patients than in controls PMID: 19377255

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  • 亞細(xì)胞定位：
    [Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic]: Cytoplasm.
  • 蛋白家族：
    Class-I pyridine nucleotide-disulfide oxidoreductase family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 4623

    OMIM: 138300

    KEGG: hsa:2936

    STRING: 9606.ENSP00000221130

    UniGene: Hs.271510