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  4. Human Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

Human Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

  • 中文名稱：
    人XIIA組分泌型磷脂酶A2(PLA2G12A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018086HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人XIIA組分泌型磷脂酶A2(PLA2G12A)酶聯(lián)免疫試劑盒（CSB-EL018086HU）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PLA2G12A含量。PLA2G12A是一種分泌型磷脂酶A2，具有低活性，主要在心臟、骨骼肌、腎臟、肝臟和胰腺中表達(dá)。其研究機(jī)制涉及磷脂代謝和類二十烷酸的產(chǎn)生，與多種生理和病理作用相關(guān)，如心血管疾病和神經(jīng)發(fā)育障礙。試劑盒檢測范圍為4.7 pg/mL-300 pg/mL，該產(chǎn)品可應(yīng)用于細(xì)胞脂質(zhì)代謝研究、炎癥相關(guān)信號通路探索、疾病動物模型生物標(biāo)志物分析等科研場景，為探究PLA2G12A在病理生理過程中的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    EC 3.1.1.4 ELISA Kit; Group XII secreted phospholipase A2 ELISA Kit; Group XIIA secreted phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 precursor ELISA Kit; GXII ELISA Kit; GXII sPLA2 ELISA Kit; PG12A_HUMAN ELISA Kit; phosphatidylcholine 2 acylhydrolase 12A ELISA Kit; Phosphatidylcholine 2 acylhydrolase GXII ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit; Phospholipase A2 group XIIA ELISA Kit; PLA2G12 ELISA Kit; Pla2g12a ELISA Kit; ROSSY ELISA Kit; sPLA2 XII ELISA Kit; sPLA2-XII ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    4.7 pg/mL-300 pg/mL
  • 靈敏度：
    1.2 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:20 Average % 93    
    Range % 90-96    
    1:40 Average % 107    
    Range % 103-110    
    1:80 Average % 86    
    Range % 83-90    
    1:160 Average % 101    
    Range % 98-106    
  • 回收率：
    The recovery of human PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 85 80-90    
    EDTA plasma (n=4) 101 97-106    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average      
    300 0.236 0.230 0.233      
    150 0.386 0.391 0.389      
    75 0.589 0.592 0.591      
    37.5 0.847 0.834 0.841      
    18.75 1.076 1.100 1.088      
    9.4 1.534 1.502 1.518      
    4.7 1.952 1.991 1.972      
    0 2.345 2.401 2.373      
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
  • 基因功能參考文獻(xiàn)：
    1. The study demonstrated that PLA2G12A SNPs or haplotypes might influence the susceptibility to schizophrenia in the Han Chinese population from Northeast China. PMID: 27434078
    2. cellular arachidonate (AA) release and prostaglandin (PG) production are regulated by novel classes of secretory phospholipase A(2)s (sPLA(2)s), groups III and XII PMID: 12522102
  • 亞細(xì)胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    Phospholipase A2 family
  • 組織特異性：
    Abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18554

    OMIM: 611652

    KEGG: hsa:81579

    STRING: 9606.ENSP00000243501

    UniGene: Hs.389452