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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human HERV-K_7p22.1 provirus ancestral Env polyprotein(ERVK6) ELISA kit

Human HERV-K_7p22.1 provirus ancestral Env polyprotein(ERVK6) ELISA kit

  • 中文名稱：
    人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL007812HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶聯(lián)免疫試劑盒（CSB-EL007812HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ERVK6含量。人HERV-K_7p22.1前病毒祖先Env多蛋白（ERVK6）是內(nèi)源性逆轉(zhuǎn)錄病毒K組的一個(gè)成員，其Env蛋白在早期感染中參與受體識(shí)別和膜融合。ERVK6蛋白在血清、血漿和組織勻漿中定量測(cè)量，具有潛在的研究?jī)r(jià)值。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于體外樣本中ERVK6蛋白表達(dá)水平的精準(zhǔn)分析，可為病毒進(jìn)化研究、宿主基因組穩(wěn)定性探索以及相關(guān)疾病模型的分子機(jī)制解析提供技術(shù)支持。該產(chǎn)品嚴(yán)格適用于科研場(chǎng)景，包括但不限于細(xì)胞實(shí)驗(yàn)、動(dòng)物模型或生物樣本庫(kù)中ERVK6蛋白的動(dòng)態(tài)監(jiān)測(cè)，為分子生物學(xué)及病毒學(xué)領(lǐng)域的研究者提供穩(wěn)定可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    ERVK-6 ELISA Kit; ERVK6Endogenous retrovirus group K member 6 Env polyprotein ELISA Kit; EnvK2 protein ELISA Kit; Envelope polyprotein ELISA Kit; HERV-K(C7) envelope protein ELISA Kit; HERV-K(HML-2.HOM) envelope protein ELISA Kit; HERV-K108 envelope protein ELISA Kit; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein ELISA Kit; SU); Transmembrane protein ELISA Kit; TM)] ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ERVK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 91-99
    1:2 Average % 103
    Range % 98-106
    1:4 Average % 110
    Range % 106-114
    1:8 Average % 92
    Range % 89-97
  • 回收率：
    The recovery of human ERVK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 84 81-89
    EDTA plasma (n=4) 97 93-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.251 2.301 2.276 2.154
    5 1.375 1.368 1.372 1.250
    2.5 0.782 0.778 0.780 0.658
    1.25 0.503 0.511 0.507 0.385
    0.625 0.325 0.336 0.331 0.209
    0.312 0.247 0.268 0.258 0.136
    0.156 0.192 0.186 0.189 0.067
    0 0.121 0.123 0.122 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：人

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： 挺好的，靈敏度很高，精確度也高，實(shí)驗(yàn)結(jié)果很好，是陽(yáng)性數(shù)據(jù)，擬合曲線也挺好，試劑盒很好用，操作挺簡(jiǎn)單的

By 肖老師

靶點(diǎn)詳情

  • 功能：
    Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties.; SU mediates receptor recognition.; TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
  • 亞細(xì)胞定位：
    [Transmembrane protein]: Cell membrane; Single-pass type I membrane protein.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Endogenous retrovirus group K member 6 Env polyprotein]: Virion.
  • 蛋白家族：
    Beta type-B retroviral envelope protein family, HERV class-II K(HML-2) env subfamily
  • 組織特異性：
    Expressed in testis, and peripheral blood lymphocytes.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 13915

    OMIM: 605626