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中文名稱:人HLAⅡ類組織相容性抗原,DRα鏈(HLA-DRA)酶聯(lián)免疫試劑盒
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貨號:CSB-EL010497HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人HLAⅡ類組織相容性抗原,DRα鏈(HLA-DRA)酶聯(lián)免疫試劑盒(CSB-EL010497HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HLA-DRA含量。HLA - DRA是一種重要靶點。它屬于人類白細(xì)胞抗原基因家族,在免疫系統(tǒng)中發(fā)揮關(guān)鍵作用,參與抗原呈遞過程。目前研究圍繞其在自身免疫疾病、腫瘤免疫等方面的機制,旨在明確其作用途徑,為相關(guān)疾病的診斷、治療及藥物研發(fā)提供依據(jù)。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL,適用于基礎(chǔ)免疫學(xué)研究、疾病發(fā)生機制探索以及生物治療藥物(如免疫調(diào)節(jié)劑或單克隆抗體)的體外效果評估,為科研人員提供穩(wěn)定可靠的定量分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:DASS-397D15.1 ELISA Kit; DR alpha chain ELISA Kit; DR alpha chain precursor ELISA Kit; DRA_HUMAN ELISA Kit; DRB1 ELISA Kit; DRB4 ELISA Kit; FLJ51114 ELISA Kit; Histocompatibility antigen HLA DR alpha ELISA Kit; Histocompatibility antigen HLA-DR alpha ELISA Kit; HLA class II histocompatibility antigen ELISA Kit; HLA class II histocompatibility antigen DR alpha chain ELISA Kit; HLA DR1B ELISA Kit; HLA DR3B ELISA Kit; HLA DRA ELISA Kit; HLA DRA1 ELISA Kit; HLA DRB1 ELISA Kit; HLA DRB3 ELISA Kit; HLA DRB4 ELISA Kit; HLA DRB5 ELISA Kit; HLA-DR histocompatibility type ELISA Kit; HLA-DRA ELISA Kit; HLADR4B ELISA Kit; HLADRA1 ELISA Kit; HLADRB ELISA Kit; Major histocompatibility complex class II DR alpha ELISA Kit; Major histocompatibility complex class II DR beta 1 ELISA Kit; Major histocompatibility complex class II DR beta 3 ELISA Kit; Major histocompatibility complex class II DR beta 4 ELISA Kit; Major histocompatibility complex class II DR beta 5 ELISA Kit; MGC117330 ELISA Kit; MHC cell surface glycoprotein ELISA Kit; MHC class II antigen DRA ELISA Kit; MHC II ELISA Kit; MLRW ELISA Kit; OTTHUMP00000029406 ELISA Kit; OTTHUMP00000029407 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:18.75 pg/mL-1200 pg/mL
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靈敏度:4.68 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-DRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 84-98 1:2 Average % 91 Range % 85-95 1:4 Average % 96 Range % 92-102 1:8 Average % 103 Range % 96-108 -
回收率:
The recovery of human HLA-DRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 88-96 EDTA plasma (n=4) 96 90-101 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1200 2.247 2.235 2.241 2.121 600 1.492 1.589 1.541 1.421 300 0.974 0.999 0.987 0.867 150 0.622 0.632 0.627 0.507 75 0.432 0.412 0.422 0.302 37.5 0.289 0.310 0.300 0.180 18.75 0.208 0.198 0.203 0.083 0 0.121 0.119 0.120 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Therapeutic effects of simvastatin combined with kallistatin treatment for pediatric burn patients with sepsis Dai Y,Experimental and therapeutic medicine,2023
相關(guān)產(chǎn)品
靶點詳情
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功能:An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.
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基因功能參考文獻:
- This study demonstrated that only the non-suicidal depressed subgroup revealed significantly lower microglial reaction, i.e., a decreased density of HLA-DR positive microglia versus both depressed suicide victims and controls. PMID: 28229240
- Low HLA-DRA expression is associated with sepsis. PMID: 28771573
- In this prospective study we evaluated dynamic changes in monocyte HLA-DR expression during sepsis in relation to changes in HLA-DRA gene expression and Class II transactivator (CIITA), measured by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). PMID: 27144640
- Case-control study in Han Chinese from the southwest of the country suggests that risk of ALS is increased by the AA genotype at rs9268856 in the HLA-DRA/HLA-DRB5 gene, but not by the SNPs rs9268877 in HLA-DRA/HLA-DRB5, rs1980493 in BTNL2 or rs302668 in RAB38/CTSC PMID: 28131168
- Kaposi's sarcoma-associated herpesvirus RTA not only downregulated HLA-DRalpha at the protein level through direct binding and degradation through the proteasome pathway but also indirectly downregulated the protein level of HLA-DRalpha by enhancing the expression of MARCH8, a member of the membrane-associated RING-CH (MARCH) proteins. PMID: 27356905
- The frequency of CD14(+)HLA-DR-/low MDSCs could be considered as a poor prognostic predictor in small-cell lung cancer (SCLC) and the elimination of MDSCs during medical interventions may improve the prognosis of SCLC patients. PMID: 25792471
- This meta-analysis showed that the HLA-DRA rs3129882 A/G polymorphism was not a risk factor for Parkinson's disease in Chinese. PMID: 24849299
- CD14+HLA-DR(-/low) cells were significantly increased in gastric cancer tissue and were shown to have a critical role in CD4+T-cell immunosuppression. PMID: 25695487
- No significant association was found for HLA-DRA/PARK18 rs3129882 polymorphism and Parkinson's disease risk (meta-analysis). PMID: 25720714
- The results suggest that rs3129882 polymorphism in HLA-DR may be a risk factor for Parkinson's disease in Iranian. PMID: 25319953
- Increased CD14+ monocytes with loss of HLA-DR expression were seen in patients with higher stage disease, more aggressive pathology, and in relapse or refractoriness to treatment. PMID: 24636145
- HLA-DRA variants predict penicillin allergy in genome-wide fine-mapping genotyping PMID: 25224099
- The effects of rs11248051 and rs1564282 variants of GAK, and the rs3129882 variant of HLA-DRA, were investigated in Parkinson's disease patients. PMID: 24039160
- The SNP rs3129882 in intron 1 of HLA-DRA has been shown to be most robustly associated with the Parkinson's disease. PMID: 23083294
- suggest that binding of MAM to HLA-DR leads to a conformational change in MAM structure allowing its interaction with TLR2 and TLR4 and a better recognition by T cells. PMID: 24493819
- The HLA-DRA variation rs3129882 is not associated with Parkinson's disease in Sweden.( PMID: 23579001
- The expression of human leucocyte antigen (HLA)-DR in epithelial cells and cluster of differentiation (CD8)-positive lymphocytes as possible markers of chronic ocular graft versus host disease, was investigated. PMID: 23878500
- HLA-DR4, PAD4 and STAT4 are overexpressed in rheumatoid arthritis and may be involved in the pathogenesis of RA. PMID: 20584675
- 2 SNPs in the HLA-DRA gene were studied in Caucasian patients from 2 large studies of alcohol dependence. rs2239803 & rs4935356 were associated with AD in both cohorts. PMID: 22890421
- Data indicate that 25 +/- 1.3% of CD74 and 17 +/- 0.3% of HLA-DR are colocalized. PMID: 22889831
- In this study observe no association between this single nucleotide polymorphism of HLA-DRA and Parkinson's disease in a Taiwanese population. PMID: 22243834
- genetic polymorphism is associated with the presence of nasal polyposis in asthmatic patients PMID: 22391069
- The rs3129882 noncoding variant in intron 1 of HLA-DRA is associated with late-onset sporadic Parkinson disease in a Chinese Han population. PMID: 21791235
- small interfering RNA-mediated knock-down of AIM2 resulted in reduced expression of HLA-DRA, HLA-DRB and CIITA in IFN-gamma-treated cells PMID: 21804607
- SNP1 is a Parkinson's disease-associated variant in HLA-DRA and is associated with HLA-DRA, DRB5 and DQA2 gene expression. PMID: 22096524
- Molecular Dynamics simulations of the MuBetaRho 83-99 (Phe91) and MuBetaRho 83-99 (Tyr91) peptide analogues in complex with HLA-DR2 (DRA, DRB1*1501) and T-cell receptors were performed. PMID: 21898163
- This study indicated that the association between rs3129882 and parkinson disease remains unclear and requires further study. PMID: 21482477
- HLA-DR(+) endothelial cells regulate the local inflammatory allogeneic response, promoting either an IL-6/STAT-3-dependent Th17 response or a contact-CD54-dependent regulatory response according to the cytokine environment. PMID: 21282653
- Upregulation of HLA-DRA is associated with acute pancreas allograft rejection. PMID: 20497195
- The skeletal muscles of myasthenia gravis up regulation of MHC class @@. PMID: 20546939
- predictive value of expression in diagnosis of acute promyelocytic leukemia PMID: 20232575
- Continuous veno-venous hemofiltration is effective in removal of many plasma cytokines and in improvement of monocyte HLA-DR expression in septic patients. PMID: 20202859
- DNMT1 and HLA-DRalpha are prognostic factors for hepatocelluar carcinoma. PMID: 18931722
- Data show that pollen grains triggered the production of IL-8, TNF-alpha, IL-6 and strongly upregulated the membrane expression of CD80, CD86, CD83, HLA-DR and caused only a slight increase in the expression of CD40. PMID: 20118277
- Findings show that Sug1 is crucial for regulating histone H3K4me3 and H3R17me2 at the cytokine inducible MHC-II and CIITA promoters. PMID: 19660582
- Studies indicate that five SNPs showed genome-wide significant association with MS: HLA-DRA, IL7R, IL2RA, CD58 and CLEC16A. PMID: 19834503
- P. gingivalis membrane vesicles apparently inhibited IFN-gamma-induced MHC class II (HLA-DRalpha) by disrupting the IFN-gamma signaling transduction pathway. PMID: 11854199
- IFN-gamma-induced HLA-DRA expression was inhibited by nitric oxide (NO) and antioxidants such as superoxide dismutase, catalase, pyrrolidine dithiocarbamate, and N-acetylcysteine PMID: 12006557
- The donor TNFRSF6 polymorphism dierctly or indirectly influences acute kidney rejection episodes. PMID: 14736971
- different regions of the cytoplasmic tails are involved in the association and the recruitment of HLA-DR(A & B1) into different compartments. HLA-DP and -DR interact differently with the cytoskeleton. PMID: 14976194
- Data show that Oct-1 occupies the endogenous HLA-DRA promoter when the HLA-DRA promoter is inactive in retinoblastoma protein-defective cells. PMID: 15105429
- Overexpression of HLA-DRA is associated with ovarian and other cancers PMID: 15467430
- Downregulation of HLA-DRA is associated with early intrahepatic recurrence of hepatocellular carcinoma PMID: 15688398
- Alleles of IL2RA and IL7RA and those in the HLA locus are identified as heritable risk factors for multiple sclerosis. PMID: 17660530
- HLA-DR alpha 2 domain (sHLA-DRalpha2) induces negative signals by engaging TIRC7 on lymphocytes, inhibiting proliferation and inducing apoptosis in CD4+ and CD8+ T-cells via activation of the intrinsic pathway PMID: 18270567
- although lysine 219 is absolutely required for modification of DRalpha, other features of the DRalpha tail act to limit the extent of ubiquitination. PMID: 19117940
- T cell receptor view of HLA-DRA in pocket 9 shows that the arrangement of residues in this pocket promotes anchoring of either large aliphatic or aromatic amino acids at this position. PMID: 19648278
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亞細(xì)胞定位:Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Autolysosome membrane; Single-pass type I membrane protein.
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蛋白家族:MHC class II family
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組織特異性:Expressed in professional APCs: macrophages, dendritic cells and B cells (at protein level). Expressed in thymic epithelial cells (at protein level).
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