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  4. Human Heat shock protein beta-2(HSPB2) ELISA kit

Human Heat shock protein beta-2(HSPB2) ELISA kit

  • 中文名稱：
    人熱休克蛋白β-2(HSPB2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL010835HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人熱休克蛋白β-2(HSPB2)酶聯(lián)免疫試劑盒（CSB-EL010835HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HSPB2含量。HSPB2屬于小分子熱休克蛋白家族。其背景上，它在多種組織中表達，與細胞應(yīng)激保護、凋亡調(diào)控等密切相關(guān)。研究機制方面，主要通過與其他蛋白相互作用來穩(wěn)定蛋白結(jié)構(gòu)、調(diào)節(jié)細胞信號通路，參與維持細胞內(nèi)環(huán)境穩(wěn)定，相關(guān)研究聚焦其在疾病發(fā)生發(fā)展中的作用。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于基礎(chǔ)科研中探究HSPB2在應(yīng)激反應(yīng)調(diào)控、疾病模型構(gòu)建或藥物干預(yù)實驗中的動態(tài)表達，例如阿爾茨海默病、心肌缺血等疾病機制研究，或作為生物標(biāo)志物在相關(guān)分子通路分析中的應(yīng)用本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    DMPK-binding protein ELISA Kit; Heat shock 27kD protein 2 ELISA Kit; Heat shock 27kDa protein 2 ELISA Kit; Heat shock protein beta-2 ELISA Kit; Heat-shock protein beta-2 ELISA Kit; Hs.78846 ELISA Kit; HSP27 ELISA Kit; HspB2 ELISA Kit; HSPB2_HUMAN ELISA Kit; LOH11CR1K ELISA Kit; MGC133245 ELISA Kit; MKBP ELISA Kit
  • 縮寫：
    HSPB2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HSPB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 107
    Range % 100-111
    1:2 Average % 99
    Range % 91-103
    1:4 Average % 94
    Range % 87-100
    1:8 Average % 102
    Range % 94-107
  • 回收率：
    The recovery of human HSPB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 89-93
    EDTA plasma (n=4) 98 94-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.526 2.601 2.564 2.405
    10 2.115 2.056 2.086 1.927
    5 1.417 1.403 1.410 1.251
    2.5 0.838 0.854 0.846 0.687
    1.25 0.503 0.489 0.496 0.337
    0.625 0.325 0.309 0.317 0.158
    0.312 0.245 0.234 0.240 0.081
    0 0.161 0.157 0.159 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    May regulate the kinase DMPK.
  • 基因功能參考文獻：
    1. in differentiating myoblasts, nuclear HSPB2 compartments sequester lamin A. PMID: 28854361
    2. HSPB2 competes with HSPB8 for binding to BAG3. In contrast, HSPB3 negatively regulates HSPB2 association with BAG3. PMID: 28181153
    3. HspB2 has roles in maintaining ATP levels and as well as chaperone-dependent functions for metabolic homeostasis PMID: 26465331
    4. Results suggest a limited function of alphaB-crystallin and HSP27 in preventing abnormal tau protein deposition in glial cells and neurons; in addition, the expression of alphaB-crystallin phosphorylated at Ser59 may act as a protective factor in glial cells. PMID: 24985029
    5. These findings provide novel insights into the role of p53 as a regulator of bidirectional gene pair HspB2/alpha B-crystallin-mediated ROS and the Warburg effect PMID: 24859470
    6. Study shows that HspB2 oligomers exchange subunits. PMID: 22272249
    7. promoter methylation of heat shock protein B2 is a novel molecular biomarker in human esophageal squamous cell carcinoma PMID: 21258768
    8. Reveal a novel function of HspB2 as an anti-apoptotic protein that negatively regulates apical caspase 3/8 activation in the extrinsic apoptotic pathway. PMID: 20087649
    9. Hsp27, but not the classic Hsp60 and Hsp70, may be associated with the development bronchiolitis obliterans syndrome. PMID: 20456980
    10. Sodium salicylate can induce the expression of HSP27 in human lens epithelial cells. PMID: 19513626
    11. analysis of CEA-, Her2/neu-, BCRP- and Hsp27-positive microparticles in breast cancer patients PMID: 20592365
    12. HSP27 is linked to multi-drug resistance in cell line HepG2/VCR. PMID: 17524270
    13. The abnormal expression of 14-3-3 sigma and HSP27 is significantly associated with lymph node metastasis in colorectal cancer. PMID: 20336542
    14. 5-fluorouracil & carboplatin specifically induce expression of Hsp27 in hepatoma cells. siRNA knockdown of Hsp27 diminishes survival of drug-exposed cells. PMID: 19901540
    15. HSP27 confers reresistance to androgen ablation and chemotherapy in prostate cancer cells through eIF4E PMID: 20101233
    16. PM induces ROS generation in human lung endothelium, resulting in oxidative stress-mediated EC barrier disruption via p38 MAPK- and HSP27-dependent pathways. PMID: 19520919
    17. study indicate that monocytes do not trigger apoptosis of vascular endothelial cells; proapoptotic activation mediated by PUMA seemed to be counterbalanced by significant increase of antiapoptotic HSP70, HSP27 and especially phospho-HSP27 proteins level PMID: 20065497
    18. HSP27 and p53 protein levels play a key role(s) in heat shock-mediated switch of glucose depreivation-induced necrosis to apoptosis. PMID: 20043073
    19. Small interfering RNA-mediated silencing of heat shock protein 27 (HSP27) Increases chemosensitivity to paclitaxel by increasing production of reactive oxygen species in ovarian cancer. PMID: 19930842
    20. HSP27 and alphaB-crystallin are increased, phosphorylated and localized in aggresomes when proteasome activity is inhibited PMID: 11926998
    21. HSP27 nuclear staining can serve as a sensitive marker for skin irritation or cellular stress in excised skin PMID: 12473058
    22. HSP72 biosynthesis was analyzed by an image processing, computer-assisted imaging method in an endothelial vascular cell line and compared to a hand-calculated method. No differences were seen. PMID: 12503731
    23. Hsp27 regulates apoptosis through an ability to interact with key components of the apoptotic signalling pathway [review] PMID: 12510153
    24. hsp72 reduces caspase-3-mediated proteolysis of FAK, an anti-apoptotic protein PMID: 12611892
    25. Hsp27 plays a significant role in the IFN-gamma-induced sensitization of oral SCC cells to anticancer drugs PMID: 12700631
    26. Data show that heat shock protein 72 suppressed extracellular signal-regulated kinase activation by protecting dual-specificity phosphatases and suppressing MEK1/2. PMID: 12748284
    27. Associates with the I kappa B kinase complex regulates tumor necrosis factor alpha-induced NF-kappa B activation. PMID: 12829720
    28. down regulation in neuronal and non-neuronal cells expressing mutant ataxin-3 PMID: 12832059
    29. HSP27 is a ubiquitin-binding protein involved in I-kappaBalpha proteasomal degradation PMID: 12897149
    30. Phosphorylated HSP27 favors reduced bead motions that are probably due to stabilization of the actin cytoskeleton. PMID: 14729728
    31. HSP27 may provide a neuroprotective effect in AD and other tauopathies PMID: 14963027
    32. positive effect of Id-1 on TGF-beta1-induced cell motility was mediated through activation of MEK-ERK signaling pathway and subsequent phosphorylation of HSP27 PMID: 17916352
    33. a cellular defense against dysregulated proteins, in the form of Hsp27 and alphaB-crystallin, might contribute to the cell cycle reentry seen in AD cells. PMID: 18061943
    34. These data show that Hsp27 antagonizes Bax-mediated mitochondrial injury and apoptosis by promoting Akt activation via a PI3-kinase-dependent mechanism. PMID: 18299320
    35. Upon heating at 43 degrees C, Hsp27 effectively suppresses myosin subfragment 1 aggregation, and this effect is enhanced by mutations mimicking Hsp27 phosphorylation. PMID: 18387368
    36. In a large cohort of type 1 diabetic subjects, we found an independent association between serum HSP27 and distal symmetrical polyneuropathy. PMID: 18390793
    37. These findings identify a novel pathway for vascular endothelial growth factor-induced HSP27 serine 82 phosphorylation via PKC-mediated PKD activation and direct phosphorylation of HSP27 by PKD. PMID: 18440775
    38. HSP27 phosphorylation is correlated with ADP-induced platelet granule secretion. PMID: 18471985
    39. annexin II is a novel HSP27-interacted protein which is involved in UVC resistance in human cells PMID: 18494762
    40. Mechanistic in vitro studies showed upregulated HSP27 expression and secretion in human macrophages treated with estrogen or acLDL. PMID: 18566345
    41. In conclusion, despite the protective action against acute motor neuron injury, Hsp27 alone is not sufficient to protect against the chronic motor neuron injury due to the presence of mutant SOD1. PMID: 18624915
    42. Pint mutations in patients with type 2 diabetes and their families were studied. mitochondrial genes including np3316, np3394 and np3426 in the ND1 region and np3243 in the tRNA(Leu(UUR))were screened. PMID: 18701018
    43. Overexpression of HSP27 in squamous cell carcinoma of the uterine cervix: a proteomic analysis using archival formalin-fixed, paraffin-embedded issues is reported. PMID: 18755499
    44. HSP27 and HSP60 are predictors of biochemical recurrence of prostate cancer after radical prostatectomy. PMID: 19132982
    45. show that Hsp27 and actin are in the same complex in cells and that Hsp27 is important for cell motility. PMID: 19224398
    46. the differential expression patterns of alphaB-crystallin and Hsp27 indicate functional differences between these highly related proteins in placental tissues. PMID: 19238749
    47. data show islet protection in mice by human HSP27 by mitigation of apoptosis, possibly through nuclear factor kappaB regulation. PMID: 19325007
    48. Findings revealed a possible effect of HSP27 on apoptosis in metastatic HCC cells, in which HSP27 may regulate NF-kB pathway activation. PMID: 19331697
    49. findings provide the first evidence that expanded ataxin-3 interferes with Hsp27 synthesis, which may contribute to the impairment of the cells' ability to respond to stresses and trigger the progression of Machado-Joseph disease PMID: 19429074
    50. Overexpression of huHSP27 protects against hepatic injury and acute kidney injury associated with liver ischemia-reperfusion injury in mice. PMID: 19656912

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=Localizes to nuclear foci.
  • 蛋白家族：
    Small heat shock protein (HSP20) family
  • 組織特異性：
    Expressed preferentially in skeletal muscle and heart but not in the lens.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5247

    OMIM: 602179

    KEGG: hsa:3316

    STRING: 9606.ENSP00000457706

    UniGene: Hs.709660