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  4. Human Histidyl-tRNA synthetase, cytoplasmic(HARS) ELISA kit

Human Histidyl-tRNA synthetase, cytoplasmic(HARS) ELISA kit

  • 中文名稱：
    人組氨酰-tRNA合成酶,細胞質(zhì)(HARS)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL010137HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人組氨酰-tRNA合成酶,細胞質(zhì)(HARS)酶聯(lián)免疫試劑盒（CSB-EL010137HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HARS含量。HARS即組氨酰-tRNA合成酶，是一種參與蛋白質(zhì)合成的關(guān)鍵酶。研究發(fā)現(xiàn)其不僅在蛋白質(zhì)合成中起作用，還參與了多種疾病相關(guān)信號通路的調(diào)控，如與神經(jīng)退行性疾病、腫瘤等發(fā)生發(fā)展有關(guān)，針對它的研究機制或為疾病治療提供新方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎(chǔ)科研中HARS的蛋白表達分析、病理生理狀態(tài)下的動態(tài)變化監(jiān)測以及相關(guān)分子機制研究，支持生物學、藥理學等領(lǐng)域的體外實驗需求，為探索HARS在遺傳性神經(jīng)病變、炎癥反應(yīng)或代謝異常等研究場景提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    cytoplasmic ELISA Kit; EC 6.1.1.21 ELISA Kit; FLJ20491 ELISA Kit; HARS ELISA Kit; HisRS ELISA Kit; Histidine tRNA ligase, cytoplasmic ELISA Kit; histidine translase ELISA Kit; Histidine tRNA ligase ELISA Kit; Histidine--tRNA ligase ELISA Kit; Histidyl tRNA synthetase ELISA Kit; Histidyl-tRNA synthetase ELISA Kit; HRS ELISA Kit; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds ELISA Kit; SYHC_HUMAN ELISA Kit; USH3B ELISA Kit
  • 縮寫：
    HARS
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HARS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of human HARS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-101
    EDTA plasma (n=4)9287-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.183 2.198 2.191 2.066
    5001.425 1.438 1.432 1.307
    2500.864 0.878 0.871 0.746
    1250.541 0.568 0.555 0.430
    62.50.353 0.362 0.358 0.233
    31.250.277 0.268 0.273 0.148
    15.60.209 0.206 0.208 0.083
    00.126 0.123 0.125
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.
  • 基因功能參考文獻：
    1. This study suggests that the human HisRS genes, while descending from a common ancestor with dual function for both types of tRNA(His), have acquired highly specialized tRNA recognition properties through evolution. PMID: 28321488
    2. Despite the similar kinetics, differential scanning fluorimetry revealed that Y454S is less thermally stable than Wild Type HARS, and cells from Y454S patients grown at elevated temperatures demonstrate diminished levels of protein synthesis compared to those of Wild Type cells. The thermal sensitivity associated with the Y454S mutation represents a biochemical basis for understanding Usher Syndrome Type IIIB. PMID: 28632987
    3. Loss of function mutations in histidyl-tRNA synthetase cause a spectrum of inherited peripheral neuropathies PMID: 26072516
    4. Data suggest that by comparing human and trypanosomatid histidyl-tRNA synthetases (HisRS) may provide opportunities for developing specific inhibitors of Trypanosoma brucei HisRS. PMID: 25151410
    5. Secreted histidyl-tRNA synthetase splice variants elaborate major epitopes for autoantibodies in inflammatory myositis. PMID: 24898250
    6. Data indicate that higher anti-Jo1 levels were associated with disease severity in antisynthetase syndrome (ASS) patients. PMID: 24286268
    7. Non-Jo-1 anti-synAb positive patients have decreased survival compared with Jo-1 patients. PMID: 23422076
    8. Findings suggest that histidyl-tRNA synthetase (HARS) is associated with axonal peripheral neuropathy. PMID: 22930593
    9. The crystal structure of a novel splice variant of a tRNA synthetase lacking the entire catalytic domain. PMID: 22958643
    10. Although anti-Jo1 positive patients with antisynthetase syndrome share features of patients with anti-PL7/PL12 antibody, they exhibit many differences regarding clinical phenotype and long-term outcome. PMID: 22326685
    11. Study identified sequence variants in the known disease-causing genes SLC6A3 and FLVCR1, and present evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS. PMID: 22279524
    12. genomic organization of the HARS locus and mapping of transcripts originating from a bi-directional promoter controlling the differential expression of these gene PMID: 12056811
    13. Demonstrating histidyl-tRNA synthetase (Jo-1)-specific T cell responses represents a key step in establishing the hypothesis that Jo-1 drives T cell-mediated autoimmunity in Jo-1+ polymyositis. PMID: 12471150
    14. the TSG101 interaction with HRS is a crucial step in endocytic down-regulation of mitogenic signaling and this interaction may have a role in linking the functions of early and late endosomes PMID: 12802020
    15. A proteolytically sensitive conformation of HisRS exists in the lung, the target tissue associated with this autoantibody response. PMID: 17665459
    16. clinical and prognostic profiles of 45 patients displaying anti-histidyl-tRNA synthetase autoantibodies were determined PMID: 17785330

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  • 相關(guān)疾?。?/div>
    Usher syndrome 3B (USH3B); Charcot-Marie-Tooth disease 2W (CMT2W)
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-II aminoacyl-tRNA synthetase family
  • 組織特異性：
    Brain, heart, liver and kidney.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4816

    OMIM: 142810

    KEGG: hsa:3035

    STRING: 9606.ENSP00000425634

    UniGene: Hs.528050