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中文名稱:人鋸齒狀同源異型盒蛋白2(EN2)酶聯(lián)免疫試劑盒
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貨號:CSB-EL007660HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人鋸齒狀同源異型盒蛋白2(EN2)酶聯(lián)免疫試劑盒(CSB-EL007660HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的EN2含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,該產(chǎn)品適用于體外研究EN2蛋白在疾病模型中的表達變化,如腫瘤生物學機制探索、基因功能研究或藥物干預(yù)效果評估等領(lǐng)域,為科研人員提供穩(wěn)定可靠的分析工具,助力于癌癥相關(guān)分子標記物篩選及分子通路研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:EN2 ELISA Kit; Homeobox protein engrailed-2 ELISA Kit; Homeobox protein en-2 ELISA Kit; Hu-En-2 ELISA Kit
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縮寫:EN2
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Epigenetics and Nuclear Signaling
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human EN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 89-97 1:2 Average % 92 Range % 86-96 1:4 Average % 97 Range % 96-105 1:8 Average % 94 Range % 89-98 -
回收率:
The recovery of human EN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 83 80-87 EDTA plasma (n=4) 92 86-98 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 2.466 2.365 2.416 2.271 10 1.759 1.658 1.709 1.564 5 1.028 1.007 1.018 0.873 2.5 0.615 0.604 0.610 0.465 1.25 0.391 0.402 0.397 0.252 0.625 0.258 0.268 0.263 0.118 0.312 0.221 0.224 0.223 0.078 0 0.144 0.145 0.145 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Is Engrailed-2 (EN2) a truly promising biomarker in prostate cancer detection? Do Carmo Silva J, et al,Biomarkers,2019
相關(guān)產(chǎn)品
靶點詳情
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基因功能參考文獻:
- The EN2 transcription factor is a novel ovarian cancer biomarker. It demonstrates prognostic value, correlating with worse Overall Survival and Progression-free Survival. PMID: 30285763
- we confirmed that engrailed-2 is the target of hsa-miR-27b in human cytomegalovirus-infected glioma cells PMID: 28343438
- The critical role of miR212 in inhibiting prostate tumor growth was verified in xenograft models of prostate cancer. These findings highlighted the role of miR212 in PCa progression. More importantly, we speculate that EN-2 is a direct target gene of miR212. PMID: 28713997
- We identified a novel heterozygous missense variant (c.425C>G, p.Pro142Arg) in the Engrailed 2 (EN2) gene in two patients. The G variant has never been reported in public databases and was absent in 100 Thai patients with ASD and 435 Thai controls. A case-control study showed that the G allele of c.425C>G was significantly associated with ASD PMID: 27755371
- EN2 is negatively regulated by miR-605, and down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer (PCa) cells by up-regulating EN2, which leads to PCa development and progression PMID: 28943214
- Our data suggest that the functional interaction of miR-33a and EN-2 is involved in tumorigenesis of prostate cancer PMID: 27921232
- the results demonstrated that EN2 might play an anti-oncogenic role in oncogenesis and development of CCRCC, thereby maintaining the normal growth of human renal tubular epithelial cells. PMID: 26948025
- EN2 may be a candidate oncogene in bladder cancer by activating the PI3K/Akt pathway and inhibiting PTEN. PMID: 25812440
- Engrailed-2 (EN-2) is a developmentally regulated homeobox gene that is essential for Purkinje cell maturation and normal cerebellar development and patterning. PMID: 25290267
- The mean EN2 levels in prostate cancer patients were 3.76-fold higher than those in non-prostate patients after prostate massage. PMID: 25003607
- The exact role of EN2 expression in renal carcinoma carcinogenesis requires further investigation. PMID: 24532139
- En2 identified as a new regulator of T-cell differentiation whose disruption induces a malignant T-cell fate in chronic myelogenous leukemia progression. PMID: 24675889
- Two patients with underdeveloped arcuate fasciculus had novel, nonsynonymous variants in MID1 and EN2 genes regulating axon guidance pathway. PMID: 24321989
- EN2 levels are increased in affected individuals with an A-C/G-T genotype, supporting EN2 as an autism spectrum disorder susceptibility gene. PMID: 24520327
- urinary EN2 has the potential to be a more sensitive and specific protein biomarker for non-muscle-invasive bladder cancer than currently available tests. PMID: 23434148
- Urinary EN2 may be useful as a non-invasive early biomarker for prostate cancer detection in genetically high-risk individuals PMID: 23792811
- Normal EN-2 downregulation may not occur in some individuals with autism, such that the postnatal persistence of EN-2 overexpression may contribute to autism cerebellar abnormalities. PMID: 23423141
- The WNT2 gene and EN2 gene may act in concert to influence the language development in autism. PMID: 22522212
- findings show EN2 was expressed and secreted by prostate cancer (PC) cell lines and PC tissue but not by normal prostate tissue or stroma; presence of EN2 in urine was highly predictive of PC PMID: 21364037
- This study demonistrated that in Han chinese the association between the EN2 genetic polymorphism and autism. PMID: 20523082
- Positive genetic correlation of EN2 with autism in the Indian population. PMID: 20050924
- In human, failure to form a mesencephalon and metencephalon, with cerebellar hypoplasia, results drom a mutation or deletion in EN2(Engrailed-2) gene. PMID: 11815869
- EN2 exon 1 variant transmission disequilibrium test shows no association with autistic disorder nor association between language and stereotypic behaviour quantitative traits. PMID: 12525552
- An association between a cerebellar patterning gene and autism spectrum disorder suggests a role for EN2 as a susceptibility locus and supports a neurodevelopmental defect hypothesis in the etiology of autism. PMID: 15024396
- RNA interference studies suggest that EN2 expression is required for the maintenance of the transformed phenotype of a human breast tumor cell line PMID: 16007149
- Loss and gain of function experiments firmly establish that En1/2 is a true survival factor for dopaminergic neurons in vivo. PMID: 17267560
- Heterogeneous association between engrailed-2 and autism in the CPEA network PMID: 17948868
- a preferential transmission of the rs3824068 A-allele to AUTISM affected offspring PMID: 17948901
- Engrailed 2 gene is associated with autistic disorder in Chinese children. PMID: 18424904
- The majority of regulated genes were down-regulated in engrailed-2 cerebella from transgenic mice, consistent with the known repressive function of Engrailed-2. PMID: 18562208
- variations of the engrailed-2 gene are implicated in the development of young-onset Parkinson's disease PMID: 19270442
- The data of this study indicated that the A-C haplotype is functional and, together with the association and LD mapping results, supports EN2 as a likely autism susceptibility gene and the A-C haplotype as a possible risk allele. PMID: 19615670
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相關(guān)疾?。?/div>Genetic variations in EN2 may be associated with susceptibility to autism.亞細胞定位:Nucleus.蛋白家族:Engrailed homeobox family數(shù)據(jù)庫鏈接:
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