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  4. Human Hyaluronidase-2(HYAL2) ELISA kit

Human Hyaluronidase-2(HYAL2) ELISA kit

  • 中文名稱：
    人透明質(zhì)酸酶-2(HYAL2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL010919HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人透明質(zhì)酸酶-2(HYAL2)酶聯(lián)免疫試劑盒（CSB-EL010919HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HYAL2含量。HYAL2是一種靶點(diǎn)。它是透明質(zhì)酸酶家族成員，在細(xì)胞外基質(zhì)代謝等過(guò)程中有重要作用。研究發(fā)現(xiàn)其與腫瘤發(fā)生、發(fā)展等機(jī)制相關(guān)，可能參與腫瘤細(xì)胞增殖、遷移及免疫逃逸，是腫瘤治療等領(lǐng)域潛在研究靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，細(xì)胞生物學(xué)、疾病機(jī)制研究、腫瘤微環(huán)境調(diào)控、炎癥性疾病、組織纖維化研究等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Hyal 2 ELISA Kit; Hyal-2 ELISA Kit; Hyal2 ELISA Kit; HYAL2_HUMAN ELISA Kit; Hyaluronidase 2 ELISA Kit; Hyaluronidase 2 Precursor ELISA Kit; Hyaluronidase-2 ELISA Kit; Hyaluronoglucosaminidase 2 ELISA Kit; Hyaluronoglucosaminidase-2 ELISA Kit; LUCA 2 ELISA Kit; LUCA-2 ELISA Kit; LUCA2 ELISA Kit; Lung carcinoma protein 2 ELISA Kit; lysosomal hyaluronidase ELISA Kit; PH 20 homolog ELISA Kit; PH-20 homolog ELISA Kit; PH20 homolog ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HYAL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %89
    Range %84-93
    1:10Average %98
    Range %84-102
    1:20Average %84
    Range %80-88
    1:40Average %95
    Range %90-100
  • 回收率：
    The recovery of human HYAL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10399-108
    EDTA plasma (n=4)9288-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.382 2.301 2.342 2.185
    101.852 1.802 1.827 1.670
    51.170 1.195 1.183 1.026
    2.50.789 0.837 0.813 0.656
    1.250.471 0.458 0.465 0.308
    0.6250.335 0.345 0.340 0.183
    0.3120.227 0.213 0.220 0.063
    00.160 0.154 0.157
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.
  • 基因功能參考文獻(xiàn)：
    1. an important role for HYAL2 in CD44 alternative splicing. PMID: 29162741
    2. genetic variation in HYAL2 influences platelet aggregation PMID: 28300864
    3. Knocking down HYAL2 in HUVECs protected against HA degradation in the glycocalyx by inhibiting the expression and activity of HYAL2 and further blocked the dephosphorylation of eNOS-Ser-633 and the decrease in NO production in response to LSS. PMID: 27798230
    4. HYAL2 mutations identified as a cause of syndromic orofacial clefting and cor triatriatum sinister in amish families. PMID: 28081210
    5. our data uncover a previously unsuspected mechanism of how hyaluronan and Hyal-2 control platelet generation. PMID: 27398974
    6. Data show that DNA methylation at CpG island of hyaluronoglucosaminidase 2 (HYLA2) could be used to identify stage II and III colon cancer patients who are most likely to benefit from 5-flourouracil chemotherapy with respect to progression-free survival. PMID: 26453961
    7. CD44 knock-down in bovine and human chondrocytes results in release of bound HYAL2. PMID: 25864644
    8. High expression of S100P and HYAL2 is significantly associated with advanced disease and shorter survival of triple-negative breast cancer and S100P and HYAL2 could be potential prognostic markers of TNBC. PMID: 26112095
    9. HYAL2 plays a redundant role in the catalysis of megadalton HA to its 20 kDa intermediate during fertilization. PMID: 25232017
    10. Lower platelet HYAL2 levels and activity are associated with inflammatory bowel disease. PMID: 25411425
    11. A strong association between decreased HYAL2 methylation in peripheral blood and BC. PMID: 25213452
    12. Inverse expression of hyaluronidase 2 and hyaluronan synthases 1-3 is associated with reduced hyaluronan content in malignant cutaneous melanoma. PMID: 23560496
    13. HAS2-HYAL2/CD44 system may support spontaneous chemokinesis of human cancer cells through self-degradation of HMW-HA to produce LMW-HA by an autocrine mechanism. PMID: 21743962
    14. Hyaluronan synthases (HAS1-3) and hyaluronidases (HYAL1-2) in the accumulation of hyaluronan in endometrioid endometrial carcinoma PMID: 20875124
    15. Overexpression of HYAL2 is associated with colorectal cancer. PMID: 20849597
    16. ROS induce Hyal2, suggesting that Hyal2 is likely responsible for the sustained HA fragmentation in the airway lumen observed in inflammatory conditions associated with oxidative stress. PMID: 20554532
    17. Data show that a significant in expression levels of HA synthases (HASs) and hyaluronidases (Hyals) was observed in vitro on stimulation of epithelial ovarian carcinoma cells by gonadotropins. PMID: 20072653
    18. more elevated in human brain metastases than in primary brain tumours PMID: 12174938
    19. suppresses transformation by the Env proteins of jaagsiekte sheep retrovirus and enzootic nasal tumor virus in rodent fibroblasts by increasing Env degradation PMID: 12584308
    20. Hyaluronidase 2 negatively regulates RON receptor tyrosine kinase and mediates transformation of epithelial cells by jaagsiekte sheep retrovirus. PMID: 12676986
    21. Down-regulation of HYAL2 is associated with small cell lung cancer and glioma PMID: 12684632
    22. soluble active form expressed and characterized as Jaagsiekte sheep retrovirus receptor PMID: 15596803
    23. In chondrocytes, HYAL-2 appears to be constitutively expressed and not inducibly regulated by catabolic agents. As such, it appears that the expression of lysosomal hyaluronidase participates little in the overall regulation of hyaluronan catabolism. PMID: 15923194
    24. Association of hyaluronidases 1/2 expresion with neurons in the infarcted and peri-infarcted regions of the middle cerebral artery. PMID: 16837837
    25. purified human Hyal2 is a weak acid-active hyaluronidase PMID: 17229709
    26. alternative splicing variants, M-RIP, HYAL2, CDCA1, and MSMB genes showed differential expressions between cancer cells and corresponding normal tissues. PMID: 19081476
    27. Down-regulation of RBSP3/CTDSPL, NPRL2/G21, RASSF1A, ITGA9, HYAL1 and HYAL2 genes in non-small cell lung cancer PMID: 19140316
    28. HYAL1, but not HYAL2, expression is reduced and correlates with the accumulation of hyaluronan in ovarian carcinomas. PMID: 19435493
    29. platelets and megakaryocytes contain only hyaluronidase 2 (HYAL2) but not HYAL1 PMID: 19443707
    30. Similar in structure to hyaluronidases, which intracellularly degrade hyaluronan. The accumulation of hyaluronan (HA) in the renal cortex is a characteristic feature of inflammatory renal diseases and could participate in immune renal injury. PMID: 9933825

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  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Glycosyl hydrolase 56 family
  • 組織特異性：
    Widely expressed. No expression detected in adult brain.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 5321

    OMIM: 603551

    KEGG: hsa:8692

    STRING: 9606.ENSP00000350387

    UniGene: Hs.76873