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  4. Human Hyaluronidase PH-20(SPAM1) ELISA kit

Human Hyaluronidase PH-20(SPAM1) ELISA kit

  • 中文名稱：
    人透明質(zhì)酸酶PH-20(SPAM1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL022471HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人透明質(zhì)酸酶PH-20(SPAM1)酶聯(lián)免疫試劑盒（CSB-EL022471HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、尿液、組織勻漿樣本中的SPAM1含量。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品適用于基礎(chǔ)科研中對(duì)PH - 20的定量分析，可用于探究生殖生物學(xué)中的受精機(jī)制、腫瘤侵襲轉(zhuǎn)移中的基質(zhì)重塑作用，或評(píng)估疾病模型中透明質(zhì)酸代謝相關(guān)通路的調(diào)控機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    epididymis secretory sperm binding protein Li 96n ELISA Kit; HEL-S-96n ELISA Kit; HYA1 ELISA Kit; HYAL PH-20 ELISA Kit; HYAL PH20 ELISA Kit; Hyal-PH20 ELISA Kit; HYAL1 ELISA Kit; HYAL3 ELISA Kit; HYAL5 ELISA Kit; HYALP_HUMAN ELISA Kit; Hyaluronidase PH-20 ELISA Kit; Hyaluronoglucosaminidase PH-20 ELISA Kit; MGC26532 ELISA Kit; PH-20 Hyaluronidase ELISA Kit; PH20 ELISA Kit; PH20 Hyaluronidase ELISA Kit; SPAG15 ELISA Kit; SPAM-1 ELISA Kit; Spam1 ELISA Kit; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit; Sperm adhesion molecule 1 ELISA Kit; Sperm surface protein PH-20 ELISA Kit; Sperm surface protein PH20 ELISA Kit; zona pellucida binding ELISA Kit
  • 縮寫：
    SPAM1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 97  
    Range % 91-102  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 85  
    Range % 80-89  
  • 回收率：
    The recovery of human SPAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 104 100-108  
    EDTA plasma (n=4) 87 82-93  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.374 2.452 2.413 2.313  
    500 1.619 1.676 1.648 1.548  
    250 0.936 1.004 0.970 0.870  
    125 0.537 0.549 0.543 0.443  
    62.5 0.381 0.374 0.378 0.278  
    31.2 0.257 0.259 0.258 0.158  
    15.6 0.168 0.164 0.166 0.066  
    0 0.102 0.098 0.100    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見問(wèn)題解答
Q:

We wonder whether this kit could be used in detection of recommbinant
Hyaluronidase by CHO.
We look forward to your reply.

A:
Thanks for your inquiry!
CSB-EL022471HU Human Hyaluronidase PH-20 (SPAM1) ELISA kit
This kit is designed according to: https://www.uniprot.org/uniprot/P38567.
It detects Hyaluronidase PH-20 rather than Hyaluronidase. If you want to detect recommbinant Hyaluronidase PH-20 by CHO, sorry that we are not sure 100% if it can be detected out or not. The recombinant protein used in the kit is 293 cell derived. As it involves different expression systems and modifications after expression, we can not guarantee that it can be detected out. We suggest you do a pretest first.
Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能：
    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
  • 基因功能參考文獻(xiàn)：
    1. Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID: 25209425
    2. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID: 24778442
    3. HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID: 23247813
    4. The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID: 23209833
    5. PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID: 23463525
    6. SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID: 12932297

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  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Glycosyl hydrolase 56 family
  • 組織特異性：
    Testis.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 11217

    OMIM: 600930

    KEGG: hsa:6677

    STRING: 9606.ENSP00000345849

    UniGene: Hs.121494